A Receptor-like Cytoplasmic Kinase Targeted by a Plant Pathogen Effector Is Directly Phosphorylated by the Chitin Receptor and Mediates Rice Immunity

Yamaguchi, Koji; Yamada, Kenta; Ishikawa, Kazuya; Yoshimura, Satomi; Hayashi, Nagao; Uchihashi, Kouhei; Ishihama, Nobuaki; Kishi-Kaboshi, Mitsuko; Takahashi, Akira; Tsuge, Seiji; Ochiai, Hirokazu; Tada, Yutaka; Shimamoto, Ko; Yoshioka, Hirofumi; Kawasaki, Tsutomu

doi:10.1016/j.chom.2013.02.007

Cited by 238 publications

(272 citation statements)

References 34 publications

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“…1A). Then, by quantitative reverse transcription (qRT)-PCR, we examined the expression of five defense-related genes, Oryza sativa pathogenesisrelated protein1 (OsPR1), OsPR10, and the PTI-related genes Oryza sativa probenazole-inducible1, OsKS4, and OsNAC4 (Park et al, 2012;Yamaguchi et al, 2013). The transcripts of all five genes were significantly increased in IRBLkm-Ts and IRBLz5-CA in comparison with those in LTH (Fig.…”

Section: Identification Of the Monogenic Resistant Line With Strong Dmentioning

confidence: 99%

Multiple Rice MicroRNAs Are Involved in Immunity against the Blast Fungus Magnaporthe oryzae

et al. 2013

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MicroRNAs (miRNAs) are indispensable regulators for development and defense in eukaryotes. However, the miRNA species have not been explored for rice (Oryza sativa) immunity against the blast fungus Magnaporthe oryzae, the most devastating fungal pathogen in rice production worldwide. Here, by deep sequencing small RNA libraries from susceptible and resistant lines in normal conditions and upon M. oryzae infection, we identified a group of known rice miRNAs that were differentially expressed upon M. oryzae infection. They were further classified into three classes based on their expression patterns in the susceptible japonica line Lijiangxin Tuan Hegu and in the resistant line International Rice Blast Line Pyricularia-Kanto51-m-Tsuyuake that contains a single resistance gene locus, Pyricularia-Kanto 51-m (Pikm), within the Lijiangxin Tuan Hegu background. RNA-blot assay of nine of them confirmed sequencing results. Real-time reverse transcription-polymerase chain reaction assay showed that the expression of some target genes was negatively correlated with the expression of miRNAs. Moreover, transgenic rice plants overexpressing miR160a and miR398b displayed enhanced resistance to M. oryzae, as demonstrated by decreased fungal growth, increased hydrogen peroxide accumulation at the infection site, and up-regulated expression of defense-related genes. Taken together, our data indicate that miRNAs are involved in rice immunity against M. oryzae and that overexpression of miR160a or miR398b can enhance rice resistance to the disease.

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Section: Identification Of the Monogenic Resistant Line With Strong Dmentioning

confidence: 99%

Multiple Rice MicroRNAs Are Involved in Immunity against the Blast Fungus Magnaporthe oryzae

et al. 2013

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“…In addition to Pto and PBS1, RLCKs such as BIK1, PBL1, RIPK, and OsRLCK185 are also targeted by pathogen effector proteins (J. Liu et al, 2011;Feng et al, 2012;Yamaguchi et al, 2013), further supporting the importance of RLCKs in plant immunity.…”

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confidence: 94%

“…Another RLCK, BSK1, also interacts with FLS2 and is required for flagellin-induced ROS production (Shi et al, 2013). In rice (Oryza sativa), the RLCK OsRLCK185 associates with OsCERK1 (Yamaguchi et al, 2013). It is phosphorylated by OsCERK1 after chitin treatment and is required for chitin-induced immune responses.…”

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confidence: 99%

Two redundant receptor-like cytoplasmic kinases function downstream of pattern recognition receptors to regulate activation of SA biosynthesis in Arabidopsis

Kong

Sun

et al. 2016

Plant Physiol.

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“…OsRLCK185 indicate that phosphorylation of a cluster of Ser and Thr residues residing within the kinase activation loop are necessary for RLCK activity (Lu et al, 2010;Zhang et al, 2010;Yamaguchi et al, 2013;Ao et al, 2014). These phosphorylated residues are highly conserved among RLCK VII members Yamaguchi et al, 2013;Ao et al, 2014).…”

Section: Pbl13 Autophosphorylation Primarily Localizes To Its C-termimentioning

confidence: 99%

“…Analysis of BIK1 paralogs revealed that AvrPphB SUSCEPTIBLE1 (PBS1) and AvrPphB SUSCEPTIBLE1-LIKE1 (PBL1) function additively with BIK1 to regulate the ROS burst . RLCKs in subfamily VII have also been shown to be important for perception of chitin, a fungal MAMP Yamaguchi et al, 2013;Ao et al, 2014;Shinya et al, 2014). Rice (Oryza sativa) RLCK185, OsRLCK176, and Arabidopsis (Arabidopsis thaliana) PBL27 were also found to associate with the chitin coreceptor, indicating that RLCKs represent an important signaling module downstream of multiple PRR complexes (Yamaguchi et al, 2013;Ao et al, 2014;Shinya et al, 2014).…”

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confidence: 99%

PBL13 is a serine/threonine protein kinase that negatively regulates Arabidopsis immune responses.

et al. 2015

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Receptor-like cytoplasmic kinases (RLCKs) are a subset of plant receptor-like kinases lacking both extracellular and transmembrane domains. Some of the 46 members in the Arabidopsis (Arabidopsis thaliana) RLCK subfamily VII have been linked to plant innate immunity; however, most remain uncharacterized. Thus, multiple subfamily VII members are expected to be involved in plant immune signaling. Here, we investigate the role of AvrPphB SUSCEPTIBLE1-LIKE13 (PBL13), a subfamily VII RLCK with unique domain architecture. Unlike other characterized RLCKs, PBL13 transfer DNA insertion lines exhibit enhanced disease resistance after inoculation with virulent Pseudomonas syringae. The pbl13-2 knockout also exhibits elevated basal-level expression of the PATHOGENESIS-RELATED GENE1 defense marker gene, enhanced reactive oxygen species (ROS) burst in response to perception of bacterial microbial patterns, and accelerated flagellin-induced activation of mitogen-activated protein kinases. Recombinant PBL13 is an active kinase, and its primary autophosphorylated sites map to a 15-amino acid repeat motif unique to PBL13. Complementation of pbl13-2 with PBL13-3xFLAG converts the enhanced resistance and elevated ROS phenotypes back to wild-type levels. In contrast, kinase-dead PBL13 K111A -3xFLAG was unable to rescue pbl13-2 disease phenotypes. Consistent with the enhanced ROS burst in the pbl13-2 knockout, PBL13 is able to associate with the nicotinamide adenine dinucleotide phosphate, reduced oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN D (RBOHD) by split-luciferase complementation assay, and this association is disrupted by flagellin treatment. We conclude that the PBL13 kinase negatively regulates plant innate immunity to pathogenic bacteria and can associate with RBOHD before pathogen perception. These data are consistent with the hypothesis that PBL13 acts to prevent inappropriate activation of defense responses in the absence of pathogen challenge.

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A Receptor-like Cytoplasmic Kinase Targeted by a Plant Pathogen Effector Is Directly Phosphorylated by the Chitin Receptor and Mediates Rice Immunity

Cited by 238 publications

References 34 publications

Multiple Rice MicroRNAs Are Involved in Immunity against the Blast Fungus Magnaporthe oryzae

Multiple Rice MicroRNAs Are Involved in Immunity against the Blast Fungus Magnaporthe oryzae

Two redundant receptor-like cytoplasmic kinases function downstream of pattern recognition receptors to regulate activation of SA biosynthesis in Arabidopsis

PBL13 is a serine/threonine protein kinase that negatively regulates Arabidopsis immune responses.

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