A recipe for a good clinical pulmonary surfactant

Pérez‐Gil, Jesús

doi:10.1016/j.bj.2022.03.001

Cited by 30 publications

(27 citation statements)

References 140 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, PG is generally chosen to mimic the negatively charged lipids found in bacterial membranes (Clausell et al, 2007) and asymmetric lipopolysaccharide-phospholipid bilayers are built to mimic the outer membrane of Gram-negative bacteria (Michel et al, 2017). The extensively studied DPPC represents the paradigmatic lipid to model the lung surfactant (Perez-Gil, 2022). Microphase separated vesicles composed of PC/SM/cholesterol are proposed to mimic raftlike domains with coexisting liquid ordered (l o ) and liquid disordered (l d ) phases (Veatch and Keller, 2003).…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

Schiaffarino

González

García-Pérez

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.

show abstract

Section: Discussionmentioning

confidence: 99%

Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

Schiaffarino

González

García-Pérez

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…PA and higher amounts of DPPC may promote SLS structure in formulations that lack SP-B. ( Perez-Gil, 2022 ).…”

Section: Synthetic Lung Surfactantmentioning

confidence: 99%

“…Some recent work challenges this view, and some formulations choose to include cholesterol, such as the Forbes group of King’s College London (F-KCL in Table 5.1). ( Perez-Gil, 2022 , Hassoun et al, 2018 , Mingarro et al, 2008 ).…”

Section: Synthetic Lung Surfactantmentioning

confidence: 99%

Lung surfactant as a biophysical assay for inhalation toxicology

Liu

Sayes

2023

Current Research in Toxicology

View full text Add to dashboard Cite

“…Mass production of humanized surfactant protein however poses a major bottleneck in the development of next generation therapeutic surfactants. [ 29 ].…”

Section: Also In This Issuementioning

confidence: 99%

An area of greatest vulnerability - Recent advances in kidney injury

Kattner¹

2022

Biomedical Journal

View full text Add to dashboard Cite

A recipe for a good clinical pulmonary surfactant

Cited by 30 publications

References 140 publications

Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

Lung surfactant as a biophysical assay for inhalation toxicology

An area of greatest vulnerability - Recent advances in kidney injury

Contact Info

Product

Resources

About