A recombinant Bacille Calmette–Guérin construct expressing the Plasmodium falciparum circumsporozoite protein enhances dendritic cell activation and primes for circumsporozoite-specific memory cells in BALB/c mice

Arama, Charles; Waseem, Shahid; Fernández, Carmen; Assefaw-Redda, Yohannes; You, Liya; Rodriguez, Ariane; Radošević, Katarina; Goudsmit, Jaap; Kaufmann, Stefan H. E.; Reece, Stephen T.

doi:10.1016/j.vaccine.2011.09.054

Cited by 15 publications

(18 citation statements)

References 48 publications

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“…We demonstrate here for the first time that the rBCG strain increased macrophage phagocytic activity and induced higher production of NO, iNOS, and pro-inflammatory cytokines such as TNF-α and IL-1β than those produced by parent BCG-infected macrophages. The results are consistent with our previous finding 39 and other studies [46][47][48][49][50] demonstrating that a recombinant BCG is more potent in activating macrophages than parental BCG. These findings support our previous data showing that the rBCG vaccine capable of inducing a strong cell mediated and humoral immune responses in animal model.…”

Section: Discussionsupporting

confidence: 93%

“…A previous report showed that glycosylphosphatidylinositol (GPI) anchors of merozoite membrane proteins released from rupturing malaria-infected erythrocytes capable to induce low levels of TNF-α production in macrophages. 55 In addition, Arama et al 50 also demonstrated that rBCG expressing the circumsporozoite antigen of Plasmodium is more potent in inducing TNF-α production than the parent BCG strain. Based on these evidences, we speculated that the presence of MSP1-C antigen of P. falciparum in the rBCG strain increased the ability of the mycobacterium to stimulate higher inflammatory response in macrophages.…”

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory effects of recombinant BCG expressing MSP-1C ofPlasmodium falciparumon LPS- or LPS+IFN-γ-stimulated J774A.1 cells

Mohamad

Suppian

Nor

2014

Human Vaccines & Immunotherapeutics

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of recombinant BCG expressing MSP-1C ofPlasmodium falciparumon LPS- or LPS+IFN-γ-stimulated J774A.1 cells

Mohamad

Suppian

Nor

2014

Human Vaccines & Immunotherapeutics

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“…Having a BCG scar was found to significantly reduce the risk of death from malaria in children in Guinea-Bissau [106]. BCG has excellent adjuvant activity and has been used as a vector to deliver heterologous vaccine candidate antigens [107]. A recombinant BCG vaccine expressing circumsporozoit protein, merozoite surface protein-1, or 22 kDa of serine repeat antigen of Plasmodium species, has been shown to function as a malaria vaccine [104][105][106][107][108][109][110].…”

Section: The Non-genomic Role Of Vitamin D In Malariamentioning

confidence: 99%

“…BCG has excellent adjuvant activity and has been used as a vector to deliver heterologous vaccine candidate antigens [107]. A recombinant BCG vaccine expressing circumsporozoit protein, merozoite surface protein-1, or 22 kDa of serine repeat antigen of Plasmodium species, has been shown to function as a malaria vaccine [104][105][106][107][108][109][110]. These vaccines were demonstrated to enhance humoral and cellular immune responses, activate the inflammatory action of macrophages, and increase IFN-γ-producing cells.…”

Section: The Non-genomic Role Of Vitamin D In Malariamentioning

confidence: 99%

The role of Vitamin D in malaria

Lương

Nguyễn

2015

J Infect Dev Ctries

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An abnormal calcium-parathyroid hormone (PTH)-vitamin D axis has been reported in patients with malaria infection. A role for vitamin D in malaria has been suggested by many studies. Genetic studies have identified numerous factors that link vitamin D to malaria, including human leukocyte antigen genes, toll-like receptors, heme oxygenase-1, angiopoietin-2, cytotoxic T lymphocyte antigen-4, nucleotide-binding oligomerization domain-like receptors, and Bcl-2. Vitamin D has also been implicated in malaria via its effects on the Bacillus CalmetteGuerin (BCG) vaccine, matrix metalloproteinases, mitogen-activated protein kinase pathways, prostaglandins, reactive oxidative species, and nitric oxide synthase. Vitamin D may be important in malaria; therefore, additional research on its role in malaria is needed.

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“…Mycobacterium bovis BCG has been investigated as a live vaccine vector for a variety of human infections (20)(21)(22)(23)(24)(25), including HIV-1 infections (26)(27)(28)(29)(30). An important and attractive feature of using BCG is its long safety record as a tuberculosis (TB) vaccine, having being injected into over 3 billion people worldwide.…”

mentioning

confidence: 99%

Robust Immunity to an Auxotrophic Mycobacterium bovis BCG-VLP Prime-Boost HIV Vaccine Candidate in a Nonhuman Primate Model

Chege

Burgers

Stutz

et al. 2013

J Virol

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We previously reported that a recombinant pantothenate auxotroph of Mycobacterium bovis BCG expressing human immunodeficiency virus type 1 (HIV-1) subtype C Gag (rBCGpan-Gag) efficiently primes the mouse immune system for a boost with a recombinant modified vaccinia virus Ankara (rMVA) vaccine. In this study, we further evaluated the immunogenicity of rBCGpan-Gag in a nonhuman primate model. Two groups of chacma baboons were primed or mock primed twice with either rBCGpan-Gag or a control BCG. Both groups were boosted with HIV-1 Pr55 gag virus-like particles (Gag VLPs). The magnitude and breadth of HIV-specific cellular responses were measured using a gamma interferon (IFN-␥) enzyme-linked immunosorbent spot (ELISPOT) assay, and the cytokine profiles and memory phenotypes of T cells were evaluated by polychromatic flow cytometry. Gag-specific responses were detected in all animals after the second inoculation with rBCGpan-Gag. Boosting with Gag VLPs significantly increased the magnitude and breadth of the responses in the baboons that were primed with rBCGpan-Gag. These responses targeted an average of 12 Gag peptides per animal, compared to an average of 3 peptides per animal for the mockprimed controls. Robust responses of Gag-specific polyfunctional T cells capable of simultaneously producing IFN-␥, tumor necrosis alpha (TNF-␣), and interleukin-2 (IL-2) were detected in the rBCGpan-Gag-primed animals. Gag-specific memory T cells were skewed toward a central memory phenotype in both CD4؉ and CD8 ؉ T cell populations. These data show that the rBCGpan-Gag prime and Gag VLP boost vaccine regimen is highly immunogenic, inducing a broad and polyfunctional central memory T cell response. This report further indicates the feasibility of developing a BCG-based HIV vaccine that is safe for childhood HIV immunization.

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A recombinant Bacille Calmette–Guérin construct expressing the Plasmodium falciparum circumsporozoite protein enhances dendritic cell activation and primes for circumsporozoite-specific memory cells in BALB/c mice

Cited by 15 publications

References 48 publications

Immunomodulatory effects of recombinant BCG expressing MSP-1C ofPlasmodium falciparumon LPS- or LPS+IFN-γ-stimulated J774A.1 cells

Immunomodulatory effects of recombinant BCG expressing MSP-1C ofPlasmodium falciparumon LPS- or LPS+IFN-γ-stimulated J774A.1 cells

The role of Vitamin D in malaria

Robust Immunity to an Auxotrophic Mycobacterium bovis BCG-VLP Prime-Boost HIV Vaccine Candidate in a Nonhuman Primate Model

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