A Recombinant Vaccinia Virus Expressing Herpes Simplex Virus Type 1 Glycoprotein B Induces Cytotoxic T Lymphocytes in Mice

McLaughlin-Taylor, Elizabeth; Willey, Dru E.; Cantin, Edouard M.; Eberle, R.; Moss, Bernard; Openshaw, Harry

doi:10.1099/0022-1317-69-7-1731

Cited by 32 publications

(21 citation statements)

References 14 publications

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“…This failure to detect a gB-specific CTL response in HSV-l-immune mice is in disagreement with the studies of Blacklaws et al (1987) and McLaughlin-Taylor et al (1988). The former authors demonstrated a minor population of HSV-l-specific CTLs from CBA mice (H-2 k) which lysed autologous L cells expressing the HSV-1 KOS gB gene, whereas McLaughlin-Taylor et al (1988) reported a population of CTLs specific for the HSV-1 F gB molecule demonstrable in BALB/c (H-2 d) mice. We addressed these discrepancies by using the combined techniques of limiting dilution culture and split well analysis to determine the recognition of VgB11-infected cells by HSV-l-specific CTLs at the clonal level.…”

Section: Components Of the Immune Response Which Recognize Hsv-1 Gbcontrasting

confidence: 70%

Evaluation of Antiviral Immunity Using Vaccinia Virus Recombinants Expressing Cloned Genes for Herpes Simplex Virus Type 1 Glycoproteins

Martin

Cantin²,

Rouse³

1989

Journal of General Virology

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Section: Components Of the Immune Response Which Recognize Hsv-1 Gbcontrasting

confidence: 70%

Evaluation of Antiviral Immunity Using Vaccinia Virus Recombinants Expressing Cloned Genes for Herpes Simplex Virus Type 1 Glycoproteins

Martin

Cantin²,

Rouse³

1989

Journal of General Virology

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“…HSV-I strain F (obtained from P. G. Spear, Northwestern University, Chicago, II1., U.S.A.) was propagated and titrated on Vero cell monolayers. Recombinant vaccinia viruses vgBll, which expresses HSV-I gB (McLaughlin-Taylor et aL, 1988) and VSC8, which expresses flgalactosidase (Chakrabarti et al, 1985), were obtained from Bernard Moss (National Institute of Allergy and Infectious Diseases, Bethesda, Md., U.S.A.) and were propagated on CV1 cells.…”

Section: Methodsmentioning

confidence: 99%

“…The observed differences in lysis of two H-2 b target cell lines each infected with AdgB2 highlights another of the potential difficulties in comparing CTL data where different cell lines have been used. MC57 cells infected with a recombinant vaccinia virus, vgB11, expressing HSV-1 gB (McLaughlin-Taylor et al, 1988) were also efficiently lysed by HSVspecific CTLs (Fig. 4).…”

Section: (H-2 K) Cells Pak (H-2 B) Cells or B10d2 (H-2 D) Cellsmentioning

confidence: 99%

Cytotoxic T lymphocytes specific for herpes simplex virus (HSV) studied using adenovirus vectors expressing HSV glycoproteins

Witmer¹,

Rosenthal²,

Graham

et al. 1990

Journal of General Virology

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In previous work, we observed that H-2k-restricted herpes simplex virus (HSV)-specific cytotoxic T lymphocytes (CTLs) were effectively able to lyse transfected target cells expressing HSV glycoprotein C (gC), but not cells expressing gB, gD or gE. To confirm and extend our observations on the specificity of anti-HSV CTLs, recombinant adenovirus (Ad) vectors able to express HSV-1 gB or gC (AdgB2 or AdgC) were constructed. Syngeneic target cells infected with AdgB2 were efficiently lysed by primary H-2 b and H-2 d, but not by H-2k-restricted HSV-specific CTL. Limiting dilution studies indicated that 4 to l0 % of H2b-restricted HSV-specific CTLs recognize gB. H-2 k, H-2 b and H-2d-restricted anti-HSV-1 CTLs were unable to lyse AdgC-infected syngeneic target cells. To examine the apparent discrepancy between the previous results involving transfected H-2 k cells expressing gC and the present results involving AdgC-infected cells, gC-expressing cell lines used in previous experiments were subcloned and retested in CTL assays. DC2 cells which were lysed by HSV-specific CTLs in the previous experiments remained sensitive to anti-HSV CTLs but two other clones derived from the same transfection were not lysed. Further, L cells transfected with the gC or gD gene coupled to the mouse mammary tumour virus promoter and capable of expressing high levels of the glycoproteins following dexamethasone induction were not lysed by H-2k-restricted anti-HSV CTLs. These results suggest that HSV-specific CTLs do not recognize gC, at least when it is expressed using an Ad vector and in most transfected cell lines, whereas a significant proportion of anti-viral CTLs recognize gB presented in some but not all murine haplotypes.

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“…In the case of herpes simplex virus type 1 (HSV-1), the identity of target antigens is unresolved and controversial. For example, of the seven major glycoproteins, three have been the subject of both positive and negative findings (Glorioso et al, 1985;Blacklaws et al, 1987;Rosenthal et al, 1987;McLaughlin-Taylor et al, 1988;Martin et al, 1989). The confusion appears partly attributable to the influence of the major histocompatibility complex (MHC) class I genotype, but the particular system used to express the viral genes may also affect the outcome (Coupar et al, 1986;D.…”

Section: Introductionmentioning

confidence: 99%

Murine cytotoxic T lymphocytes specific for herpes simplex virus type 1 recognize the immediate early protein ICP4 but not ICP0

Martin

Zhu

Silverstein

et al. 1990

Journal of General Virology

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Vaccinia virus recombinants expressing the herpes simplex virus type 1 (HSV-1) genes encoding ICP0 or ICP4 were used to identify the precise target antigen(s) of murine anti-viral cytotoxic T lymphocytes (CTL) specific for the non-structural immediate early proteins. These studies revealed that HSV-l-specific CTL, restricted to class I major histocompatibility complex genes of the H-2 k haplotype but not the H-2 d or H-2 b haplotypes, would lyse autologous cells expressing ICP4. HSV-l-specific CTL derived from various mice strains failed to lyse target cells expressing ICP0. Calculation of the frequencies of H-2k-restricted virusspecific CTL demonstrated that approximately a third of the total HSV-l-specific response was directed against ICP4. Immunization of mice with either recombinant vaccinia virus or transfected L cells expressing ICP4 induced HSV-l-specific lymphoproliferation and delayed hypersensitivity but CTLs were not induced. More importantly, such immunized animals were unable to resist or control a subsequent challenge with virulent HSV-1.

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A Recombinant Vaccinia Virus Expressing Herpes Simplex Virus Type 1 Glycoprotein B Induces Cytotoxic T Lymphocytes in Mice

Cited by 32 publications

References 14 publications

Evaluation of Antiviral Immunity Using Vaccinia Virus Recombinants Expressing Cloned Genes for Herpes Simplex Virus Type 1 Glycoproteins

Evaluation of Antiviral Immunity Using Vaccinia Virus Recombinants Expressing Cloned Genes for Herpes Simplex Virus Type 1 Glycoproteins

Cytotoxic T lymphocytes specific for herpes simplex virus (HSV) studied using adenovirus vectors expressing HSV glycoproteins

Murine cytotoxic T lymphocytes specific for herpes simplex virus type 1 recognize the immediate early protein ICP4 but not ICP0

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