A recombination event in the 5′ flanking region of the Ly-6C gene correlates with impaired expression in the NOD, NZB and ST strains of mice.

Philbrick, William M.; Maher, Stephen E.; Bridgett, Margot M.; Bothwell, Alfred L.M.

doi:10.1002/j.1460-2075.1990.tb07427.x

Cited by 27 publications

(29 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous Southern blot results (34) two classes of recombinant phage were isolated. One corresponded to the Ly-6C.2 gene from 129 DNA, and the other was a new gene.…”

Section: Isolation Of the Chromosomal Ly-6i Genesupporting

confidence: 89%

“…The probe was a 1-kb EcoRI-PstI DNA fragment derived from the Ly-6C gene promoter previously described (34). By restriction analysis, two clones were found to represent a single novel Ly-6 gene family member.…”

Section: Isolation Of Phage Genomic Clonementioning

confidence: 99%

“…We have previously characterized the rearranged promoter in the Ly-6C gene in NOD mouse DNA that results in a 95% reduction of Ly-6C expression (34). However, as in the Ly-6A/E knockout mouse, this deficiency in Ly-6C has no apparent effect on the development of any lymphoid population.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression

Pflugh

Maher

Bothwell

2000

The Journal of Immunology

View full text Add to dashboard Cite

A new member of the mouse Ly-6SF, designated Ly-6I, has been isolated as a gene homologous to a segment of the Ly-6C gene. A single allelic difference in the mature protein sequence was identified, which is similar to other Ly-6SF members. Ly-6I mRNA has been detected in a wide range of tissues and cell lines, and a rabbit polyclonal Ab has been used to determine that Ly-6I protein is present at a low constitutive level on cell lines from several different lineages. In contrast to Ly-6C and Ly-6A/E, the Ly-6I gene is only weakly responsive to IFNs. Expression in vivo is most abundant on bone marrow populations and is coexpressed with Ly-6C on granulocytes and macrophages. However, Ly-6I is also expressed on immature B cell populations that do not express Ly-6C. Expression on mature B cells in spleen is uniformly low. Similarly, Ly-6I is expressed on TCRlow/int, but not TCRhigh, thymocytes. Ly-6I is re-expressed on Ly-6Chigh T cells in the periphery. Thus, Ly-6I may be a useful marker to define maturation stages of both T and B lymphocytes as well as subsets of monocytes and granulocytes.

show abstract

“…Consistent with previous Southern blot results (34) two classes of recombinant phage were isolated. One corresponded to the Ly-6C.2 gene from 129 DNA, and the other was a new gene.…”

Section: Isolation Of the Chromosomal Ly-6i Genesupporting

confidence: 89%

Section: Isolation Of Phage Genomic Clonementioning

confidence: 99%

See 1 more Smart Citation

Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression

Pflugh

Maher

Bothwell

2000

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

Section: Myeloid Precursor Populations In the Fetal Pancreasmentioning

confidence: 90%

“…In the fetal pancreas two precursor populations were present with a similar phenotype as blood monocytes. Due to a genetic abnormality of the Ly6C gene in NOD mice the expression of Ly6C is present, but significantly lower than in control mice [16].The phenotype of the two monocyte populations was further characterized using Ab against CD11c, F4/80 and CD86. In blood, Ly6C hi monocytes were CD11c low F4/80 1 CD86 low in both C57BL/6 and NOD mice (Fig.…”

mentioning

confidence: 99%

Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes

et al. 2011

View full text Add to dashboard Cite

The non-obese diabetic (NOD) mouse is a widely used animal model for the study of human diabetes. Before the start of lymphocytic insulitis, DC accumulation around islets of Langerhans is a hallmark for autoimmune diabetes development in this model. Previous experiments indicated that an inflammatory influx of these DCs in the pancreas is less plausible. Here, we investigated whether the pancreas contains DC precursors and whether these precursors contribute to DC accumulation in the NOD pancreas. Fetal pancreases of NOD and control mice were isolated followed by FACS using ER-MP58, Ly6G, CD11b and Ly6C. Sorted fetal pancreatic ER-MP58 1 cells were cultured with GM-CSF and tested for DC markers and antigen processing. CFSE labeling and Ki-67 staining were used to determine cell proliferation in cultures and tissues. Ly6C hi and Ly6C low precursors were present in fetal pancreases of NOD and control mice. These precursors developed into CD11c 1 MHCII 1 CD86 1 DCs capable of processing DQ-OVA. ER-MP58 1 cells in the embryonic and pre-diabetic NOD pancreas had a higher proliferation capacity. Our observations support a novel concept that pre-diabetic DC accumulation in the NOD pancreas is due to aberrant enhanced proliferation of local precursors, rather than to aberrant ''inflammatory infiltration'' from the circulation. IntroductionThe non-obese diabetic (NOD) mouse is used as a spontaneous model to study the development of type 1 diabetes [1]. Lymphocytes accumulate around and in the islets of Langerhans in NOD mice from around 6 weeks of age onwards, which results in the destruction of b-cells followed by a decrease in insulin production leading to diabetes. Prior to T-and B-cell accumulation the number of DCs increases in the pancreas and concentrates around the islets (from the age of 5 weeks onwards) [2,3]. DCs are potent APCs capable of stimulating both naïve and memory T cells [4]. The observation that DCs are the first immune cells to increase in number in the NOD pancreas points to a crucial role for DCs in the initiation of the islet autoimmune reaction. Such a role was recently proven by the demonstration that a temporal depletion of DCs totally abrogated the development of insulitis and diabetes in the NOD mouse model [5].Early studies have shown that BM precursors give rise to monocytes in blood, which circulate for a few days before they migrate into tissue where they develop into different types of DCs and macrophages. Blood monocytes can be subdivided into at least two subsets based on their Ly6C expression: classical and The nonclassical monocytes, which are Ly6C low , patrol the endothelium of the blood vessels and are required for rapid tissue invasion at the site of an infection [7]. Ly6C low monocytes are considered CD11c À , but some studies have reported the expression of CD11c on these cells [6,8]. Both types of monocytes are F4/80 1 and CD86 À [6].Data are accumulating on the presence of local tissue precursors for DCs and macrophages and the contribution of these precursors to DC and ma...

show abstract

A T cell activation antigen, Ly6C, induced on CD4+ Th1 cells mediates an inhibitory signal for secretion of IL-2 and proliferation in peripheral immune responses

et al. 1998

View full text Add to dashboard Cite

A T cell activation antigen, Ly6C, is considered to be involved in the autoimmunity of some autoimmune-prone mice; however, the function of Ly6C remains largely unknown. We prepared a rat anti-mouse Ly6C monoclonal antibody (mAb) (S14) that inhibits the proliferation of peripheral T cells stimulated with anti-CD3 mAb in vitro. S14 mAb, the specificity of which is confirmed by a cDNA transfectant, recognizes Ly6C antigen preferentially expressed on a part of CD8+ T cells in peripheral lymphoid organs. The immunohistochemical analysis demonstrates that Ly6C appears on CD8+ T cells in the conventional T cell-associated area of BALB/c but not of nonobese diabetic (NOD) mice, confirming the absence of Ly6C+ T cells in NOD mice. Addition of soluble S14 mAb to the culture does not influence the proliferation of T cells in vitro; however, the S14 mAb coated on the plate clearly inhibits the proliferation and IL-2 production of anti-CD3-stimulated peripheral T cells. The T cells are arrested at the transitional stage from G0/G1 to S+G2/M phases, but they are not induced to undergo apoptotic changes in vitro. This inhibitory signal provided through the Ly6C molecule inhibited IL-2 secretion in a subpopulation of the activated CD4+ T cells. Ly6C is expressed on T cell clones of both Th1 and Th2 cells, but the cytokine secretion from Th1 clones is preferentially inhibited. These results suggest that Ly6C mediates an inhibitory signal for secretion of cytokines from Th1 CD4+ T cells, potentially causing the inhibition of immune response in peripheral lymphoid tissues.

show abstract

A recombination event in the 5′ flanking region of the Ly-6C gene correlates with impaired expression in the NOD, NZB and ST strains of mice.

Cited by 27 publications

References 60 publications

Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression

Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression

Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes

A T cell activation antigen, Ly6C, induced on CD4+ Th1 cells mediates an inhibitory signal for secretion of IL-2 and proliferation in peripheral immune responses

Contact Info

Product

Resources

About