A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation

Louis, Blaise; Tewary, Mukul; Bremer, Andrew W.; Philippeos, Christina; Negri, Victor Augusti; Zijl, Sebastiaan; Gartner, Zev J.; Schaffer, David V.; Watt, Fiona M.

doi:10.1016/j.actbio.2022.07.054

Cited by 6 publications

(1 citation statement)

References 57 publications

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“…It appears that distinct populations of stem cells exist within these structures, and the differentiation pathway chosen by their progeny is largely determined by local signals from the microenvironment [ 16 ]. Understanding the factors that influence the behavior of stem cells is essential for the advancement of the field of regenerative medicine [ 17 ]. The key components of the niche are the following: homo- and heterotypic cell-cell contact, cell-extracellular matrix, adhesion, mechanical stimuli, secreted factors, and physiological factors such as oxygen and pH [ 18 ].…”

Section: Basic Features Of the Keratinization Processmentioning

confidence: 99%

Key Factors in the Complex and Coordinated Network of Skin Keratinization: Their Significance and Involvement in Common Skin Conditions

Pondeljak,

Lugović-Mihić,

Tomić

et al. 2023

IJMS

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The epidermis serves many vital roles, including protecting the body from external influences and healing eventual injuries. It is maintained by an incredibly complex and perfectly coordinated keratinization process. In this process, desquamation is essential for the differentiation of epidermal basal progenitor cells into enucleated corneocytes, which subsequently desquamate through programmed death. Numerous factors control keratinocyte differentiation: epidermal growth factor, transforming growth factor-α, keratinocyte growth factor, interleukins IL-1-β and IL-6, elevated vitamin A levels, and changes in Ca2+ concentration. The backbone of the keratinocyte transformation process from mitotically active basal cells into fully differentiated, enucleated corneocytes is the expression of specific proteins and the creation of a Ca2+ and pH gradient at precise locations within the epidermis. Skin keratinization disorders (histologically characterized predominantly by dyskeratosis, parakeratosis, and hyperkeratosis) may be categorized into three groups: defects in the α-helical rod pattern, defects outside the α-helical rod domain, and disorders of keratin-associated proteins. Understanding the process of keratinization is essential for the pathogenesis of many dermatological diseases because improper desquamation and epidermopoiesis/keratinization (due to genetic mutations of factors or due to immune pathological processes) can lead to various conditions (ichthyoses, palmoplantar keratodermas, psoriasis, pityriasis rubra pilaris, epidermolytic hyperkeratosis, and others).

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Section: Basic Features Of the Keratinization Processmentioning

confidence: 99%

Key Factors in the Complex and Coordinated Network of Skin Keratinization: Their Significance and Involvement in Common Skin Conditions

Pondeljak,

Lugović-Mihić,

Tomić

et al. 2023

IJMS

View full text Add to dashboard Cite

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Controlling Cell Interactions with DNA Directed Assembly

Mathis,

Chan,

Meckes

2024

Adv Healthcare Materials

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The creation of complex cellular environments is critical to mimicking tissue environments that will play a critical role in next‐generation tissue engineering, stem cell programming, and therapeutic screening. To address this growing need, techniques capable of manipulating cell–cell and cell‐material interactions are required that span single‐cell to 3D tissue architectures. DNA programmed assembly and placement of cells present a powerful technique for the bottom‐up synthesis of living microtissues for probing key questions in cell–cell and cell‐material‐driven behaviors through its refined control over placement and architecture. This review examines the current state of the art in the programming of cellular interactions with DNA and its applications spanning tissue model building, fundamental cellular biology, and cell manipulation for measurements across a host of applications.

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Constructing epidermal rete ridges using a composite hydrogel to enhance multiple signaling pathways for the maintenance of epidermal stem cell niche

et al. 2023

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A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation

Cited by 6 publications

References 57 publications

Key Factors in the Complex and Coordinated Network of Skin Keratinization: Their Significance and Involvement in Common Skin Conditions

Key Factors in the Complex and Coordinated Network of Skin Keratinization: Their Significance and Involvement in Common Skin Conditions

Controlling Cell Interactions with DNA Directed Assembly

Constructing epidermal rete ridges using a composite hydrogel to enhance multiple signaling pathways for the maintenance of epidermal stem cell niche

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