A regenerable screen-printed DNA biosensor based on acrylic microsphere–gold nanoparticle composite for genetically modified soybean determination

Ulianas, Alizar; Heng, Lee Yook; Ahmad, Musa; Lau, Han-Yih; Ishak, Zamri; Ling, Tan Ling

doi:10.1016/j.snb.2013.09.040

Cited by 64 publications

(29 citation statements)

References 30 publications

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“…(Amouzadeh Tabrizi and Shamsipur, ) Ulianas et al reported a storage stability of 45 days for an electrochemical biosensor with amino‐modified DNA probes covalently attached to succinimide groups of acrylic microspheres when stored in phosphate buffer at 4 °C. (Ulianas et al, ) For a successful real world application of electrochemical nucleic acid biosensors batch production of functionalised biosensors followed by long‐term dry storage ideally at room temperature is desirable if not essential for a successful market entry. To fully achieve this goal, further dedicated studies are required.…”

Section: Resultsmentioning

confidence: 99%

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Obaje

Cummins

Schulze

et al. 2016

J of Interdiscip Nanomed

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The growing threat posed by antimicrobial resistance on the healthcare and economic well‐being of mankind is pushing the need to develop novel and improved diagnostic platforms for its rapid detection at point of care, facilitating better patient management strategies during antibiotic therapy. In this paper, we present the manufacturing and characterisation of a low‐cost carbon screen‐printed electrochemical sensor on a ceramic substrate. Using label‐free electrochemical impedance spectroscopy, the sensor is demonstrated for the detection of blaNDM, which is one of the main antimicrobial resistance factors in carbapenem‐resistant Enterobacteriaceae. The electrochemical performance of the newly fabricated sensor was initially investigated in relation to the function of its underlying composite materials, evaluating the choice of carbon and dielectric pastes by characterising properties like surface roughness, wetting and susceptibility of unspecific DNA binding. Subsequently, the sensor was used in an electrochemical impedance spectroscopy assay for the sensitive and specific detection of synthetic blaNDM targets achieving a detection limit of 200 nM. The sensor properties and performance demonstrated in this study proved the suitability of the new electrode materials and manufacturing for further point‐of‐care test development as an inexpensive and effective alternative to gold electrodes sensor.

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Section: Resultsmentioning

confidence: 99%

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Obaje

Cummins

Schulze

et al. 2016

J of Interdiscip Nanomed

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“…For example, researchers used 10 % (w/v) SDS and 0.1 N HCl mixture solutions for 4 h followed by 0.5 M EDTA solution treatment for 1 h for the renewal of the SPEs surfaces 31. In another study, 0.1 M NaOH was used as regeneration solution to break the hydrogen bonds between base pairs of dsDNA to reuse the electrodes 32.…”

Section: Resultsmentioning

confidence: 99%

Prostate Cancer Biomarker Detection with Carbon Nanotubes Modified Screen Printed Electrodes

Topkaya

Ozkan-Ariksoysal

2015

Electroanalysis

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DNA hypermethylation is an epigenetic alteration and a promising biomarker for early prostate cancer detection. Simple, sensitive, easy to handle and rapid detection methodologies are imperative for point of care diagnostics especially for cancer. Herein, we describe for the first time a regenerable and compatible electrochemical biosensor for detection of Glutathione S‐Transferase P‐1 (GSTP‐1) gene hypermethylation related to prostate cancer via DNA hybridization onto the disposable Carbon and Multi Walled Carbon Nanotubes (MWCNT) Screen Printed Electrodes (SPEs). In the study, capture probes were adsorbed onto the SPEs by simple passive adsorption and then hybridization was achieved by sending the complementary target onto the probe‐modified electrodes. The selectivity of the biosensor was proved by control studies. Differential Pulse Voltammetry (DPV) technique was used to detect hybridization via guanine oxidation signals changes. The total time of the optimized method was nearly 1h, measurements took for less than 1 min, and the biosensor response was stable up to 40 days of storage period at 4 °C. The main advantages of the biosensor are very low detection limit (picomolar range) and capability of reusing the biosensor for at least 3 times after very simple regeneration process that is a unique property to reduce the cost of the assay. In addition, this is the first study that demonstrates the detection of GSTP‐1 hypermethylation electrochemically by using SPEs in order to create point of care diagnostics. The optimum parameters for the biosensor, as well as its future prospects to enhance the performance of DNA biosensors were also presented.

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“…In addition, new methods such as nano techniques (Ulianas et al, 2014), electrochemical techniques (Cagnin et al, 2009), and isothermal amplification technologies (Chen et al, 2011) are good for detecting short DNA fragments and are promising alternative or complementary methods for the detection of GM DNA in processed food. Regarding the detection of exogenous GM proteins in processed food, small peptides of the exogenous protein may be appropriate targets.…”

Section: Discussionmentioning

confidence: 99%

Degradation and detection of transgenic Bacillus thuringiensis DNA and proteins in flour of three genetically modified rice events submitted to a set of thermal processes

Wang

Chen

Dai

et al. 2015

Food and Chemical Toxicology

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A regenerable screen-printed DNA biosensor based on acrylic microsphere–gold nanoparticle composite for genetically modified soybean determination

Cited by 64 publications

References 30 publications

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Prostate Cancer Biomarker Detection with Carbon Nanotubes Modified Screen Printed Electrodes

Degradation and detection of transgenic Bacillus thuringiensis DNA and proteins in flour of three genetically modified rice events submitted to a set of thermal processes

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