A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development

Zhang, Yue; Liu, Jidan; Li, Weihua; Cao, Fangjun; Niu, Guanglin; Ji, Shengwei; Du, Xiaoyan; Cao, Binyun; An, Xiaopeng

doi:10.3389/fcell.2020.00383

Cited by 10 publications

(17 citation statements)

References 23 publications

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“…The final protein product shows different or antagonistic functional and structural characteristics compared with unspliced RNA or lead to different phenotypes due to different expression levels in the same cell ( Zhang et al, 2016 ). For example, Zang et al (2020) found that circRNAs can participate in the regulation of splicing and transcription. Chao et al (1998) found that the mouse Fmn gene can produce circRNA through “backsplicing”.…”

Section: Circular Rnasmentioning

confidence: 99%

Progress on the Regulation of Ruminant Milk Fat by Noncoding RNAs and ceRNAs

Chen

et al. 2021

Front. Genet.

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Milk fat is not only a key factor affecting the quality of fresh milk but also a major target trait forbreeding. The regulation of milk fat involves multiple genes, network regulation and signal transduction. To explore recent discoveries of pathway regulation, we reviewed the published literature with a focus on functional noncoding RNAs and epigenetic regulation in ruminants. Results indicate that miRNAs play key roles in the regulation of milk fat synthesis and catabolism in ruminants. Although few data are available, merging evidence indicates that lncRNAs and circRNAs act on milk fat related genes through indirect action with microRNAs or RNAs in the ceRNA network to elicit positive effects on transcription. Although precise regulatory mechanisms remain unclear, most studies have focused on the regulation of the function of target genes through functional noncoding RNAs. Data to help identify factors that can regulate their own expression and function or to determine whether self-regulation involves positive and/or negative feedback are needed. Despite the growing body of research on the role of functional noncoding RNA in the control of ruminant milk fat, most data are still not translatable for field applications. Overall, the understanding of mechanisms whereby miRNA, lncRNA, circRNA, and ceRNA regulate ruminant milk fat remains an exciting area of research.

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Section: Circular Rnasmentioning

confidence: 99%

Progress on the Regulation of Ruminant Milk Fat by Noncoding RNAs and ceRNAs

Chen

et al. 2021

Front. Genet.

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“…10 In addition, novel-mir-3880 played a regulatory role in breast development and milk synthesis, which showed high expression in the mammary gland of dairy goats at the peak of lactation. 11 What is more, Circ-8073, as a ceRNA, cooperated with mir-449/34 family through CEP55-FOXM1-VEGFA to regulate the establishment of milk goat receptive endometrium. 12 Based on their special regulatory function in cells, in dairy goat breeding, some miRNAs play important roles in the establishment of endometrial receptivity.…”

Section: Introductionmentioning

confidence: 99%

Effect of MiR‐100‐5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo

Zhang

Cao

et al. 2022

J Cellular Molecular Medi

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The growth of endometrial stromal cells (ESCs) at implantation sites may be a potential factor affecting the success rate of embryo implantation. Incremental proofs demonstrated that ncRNAs (e.g. miRNAs, lncRNAs and circRNAs) were involved in various biological procedures, including proliferation and apoptosis. In this study, the role of miR‐100‐5p on proliferation and apoptosis of goat ESCs in vitro and embryo implantation in vivo was determined. The mRNA expression of miR‐100‐5p was significantly inhibited in the receptive phase (RE) rather than in the pre‐receptive phase (PE). Overexpression of miR‐100‐5p suppressed ESCs proliferation and induced apoptosis. The molecular target of MiR‐100‐5p, HOXA1, was confirmed by 3′‐UTR assays. Meanwhile, the product of HOXA1 mRNA RT‐PCR increased in the RE more than that in the PE. The HOXA1‐siRNA exerted significant negative effects on growth arrest. Instead, incubation of ESCs with miR‐100‐5p inhibitor or overexpressed HOXA1 promoted the cell proliferation. In addition, Circ‐9110 which acted as a sponge for miR‐100‐5p reversed the relevant biological effects of miR‐100‐5p. The intrinsic apoptosis pathway was suppressed in ESCs, revealing a crosstalk between Circ‐9110/miR‐100‐5p/HOXA1 axis, PI3K/AKT/mTOR, and ERK1/2 pathways. To further evaluate the progress in study on embryo implantation regulating mechanism of miR‐100‐5p in vivo, the pinopodes of two phases were observed and analysed, suggesting that, as similar as in situ, miR‐100‐5p was involved in significantly regulating embryo implantation in vivo. Mechanistically, miR‐100‐5p performed its embryo implantation function through regulation of PI3K/AKT/mTOR and ERK1/2 pathways by targeting Circ‐9110/miR‐100‐5p/HOXA1 axis in vivo.

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“…Breastfeeding is the best source of nutrition for infants [ 2 ]; therefore, it is important to prevent mammary gland involution. MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs with a length of 18–23 nucleotides that play essential roles in lactation [ 3 , 4 ]. Chi-miR-8516 is one of the miRNAs that has been reported to show higher expression in the common milk stage, compared to colostrum stage [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells

Zhang

Liu

et al. 2021

Genes

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MicroRNAs play an essential role in mammary gland development, and involution is a factor that limits lactation. Chi-miR-8516 is one of the validated microRNAs that regulates the expression of STC1 and MMP1, which surge during the involution of the mammary gland. This study aims to explore the direct or indirect regulation of STC1 and MMP1 by chi-miR-8516 and the regulation of chi-miR-8516 by circ-140. In goat mammary epithelial cells, we found that chi-miR-8516 takes circ-140 as a sponge and regulates MMP1 expression by targeting STC1 and promoting the phosphorylation of MAPK. The examination of αs1-/β-casein and lipid showed the modulation of the circ-140/chi-miR-8516/STC1-MMP1 axis in casein secretion and lipid formation, which was regulated by the phosphorylation of mTOR and STAT5. This study illustrates an axis that regulates the synthesis of milk components, and explores the pathways in which the axis participates.

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A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development

Cited by 10 publications

References 23 publications

Progress on the Regulation of Ruminant Milk Fat by Noncoding RNAs and ceRNAs

Progress on the Regulation of Ruminant Milk Fat by Noncoding RNAs and ceRNAs

Effect of MiR‐100‐5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo

Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells

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