A relationship between phages and organic carbon in wastewater treatment plant effluents

Modin, Oskar; Fuad, Nafis; Abadikhah, Marie; I'Ons, David; Ossiansson, Elin; Gustavsson, David; Edefell, Ellen; Suarez, Carolina; Persson, Frank; Wilén, Britt‐Marie

doi:10.1016/j.wroa.2022.100146

Cited by 11 publications

(2 citation statements)

References 58 publications

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“…Many bacteriophages and bacteria are present in WWTPs. 41 The concentration of bacteriophages is higher in the effluent than in the influent, 42 and if phage infections frequently occur in WWTPs, a large amount of exDNA is released. However, host information is currently unknown for many viruses.…”

Section: Environmental Science: Water Research and Technology Papermentioning

confidence: 99%

Highly sensitive detection and quantification of dissolved free extracellular DNA using colloid adsorption and foam concentration

Tamai

Ogura

Okuno

et al. 2023

Environ. Sci.: Water Res. Technol.

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Section: Environmental Science: Water Research and Technology Papermentioning

confidence: 99%

Highly sensitive detection and quantification of dissolved free extracellular DNA using colloid adsorption and foam concentration

Tamai

Ogura

Okuno

et al. 2023

Environ. Sci.: Water Res. Technol.

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“…It thus follows that environmental parameters which influence host abundance are important for predatory phage success. For example, phage abundance can be influenced by the presence of essential microbial nutrients and energy sources required for host growth [ 11 , 12 ] with the virus to microbial ratio increasing with microbial cell density [ 12 ]. Thus, in soils, because particles of detrital carbon or root exudates have higher microbial densities these should be hotspots of phage predation.…”

Section: Introductionmentioning

confidence: 99%

Geochemical constraints on bacteriophage infectivity in terrestrial environments

Carlson,

Piya,

Moore

et al. 2023

ISME Communications

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Lytic phages can be potent and selective inhibitors of microbial growth and can have profound impacts on microbiome composition and function. However, there is uncertainty about the biogeochemical conditions under which phage predation modulates microbial ecosystem function, particularly in terrestrial systems. Ionic strength is critical for infection of bacteria by many phages, but quantitative data is limited on the ion thresholds for phage infection that can be compared with environmental ion concentrations. Similarly, while carbon composition varies in the environment, we do not know how this variability influences the impact of phage predation on microbiome function. Here, we measured the half-maximal effective concentrations (EC50) of 80 different inorganic ions for the infection of E. coli with two canonical dsDNA and ssRNA phages, T4 and MS2, respectively. Many alkaline earth metals and alkali metals enabled lytic infection but the ionic strength thresholds varied for different ions between phages. Additionally, using a freshwater nitrate-reducing microbiome, we found that the ability of lytic phages to influence nitrate reduction end-products depended upon the carbon source as well as ionic strength. For all phage:host pairs, the ion EC50s for phage infection exceeded the ion concentrations found in many terrestrial freshwater systems. Thus, our findings support a model where phages most influence terrestrial microbial functional ecology in hot spots and hot moments such as metazoan guts, drought influenced soils, or biofilms where ion concentration is locally or transiently elevated and nutrients are available to support the growth of specific phage hosts.

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Die unentdeckte Vielfalt der Comamonadaceae-Phagen in Kläranlagen

Barrero-Canosa,

Cooper

2024

Biospektrum

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Phages are important members of wastewater treatment plant communities. Surprisingly, little is known about phages and their impact on microbially-mediated processes in these facilities. We explore phages infecting the Comamonadaceae, a bacterial family consistently found in wastewater treatment plants. Our findings reveal limited genetic similarity between newly isolated phages and known ones, emphasizing underestimated diversity and understanding of their impact on wastewater treatment.

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A relationship between phages and organic carbon in wastewater treatment plant effluents

Cited by 11 publications

References 58 publications

Highly sensitive detection and quantification of dissolved free extracellular DNA using colloid adsorption and foam concentration

Highly sensitive detection and quantification of dissolved free extracellular DNA using colloid adsorption and foam concentration

Geochemical constraints on bacteriophage infectivity in terrestrial environments

Die unentdeckte Vielfalt der Comamonadaceae-Phagen in Kläranlagen

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