A relevant IgE-reactive 28 kDa protein identified from Salsola kali pollen extract by proteomics is a natural degradation product of an integral 47 kDa polygalaturonase

Mas, Salvador; Oeo-Santos, Carmen; Cuesta‐Herranz, Javier; Díaz‐Perales, Araceli; Colás, Carlos; Fernández, J.; Barber, Domingo; Rodrı́guez, Rosalı́a; Rı́os, Vivian de los; Barderas, Rodrigo; Villalba, Mayte

doi:10.1016/j.bbapap.2017.05.007

Cited by 13 publications

(22 citation statements)

References 33 publications

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“…In a previous report, cross-reactivity between the allergenic PG from S. kali and several members of the Oleaceae family, especially with olive pollen, was observed [6]. Therefore, we decided to study the presence of PGs in olive pollen and determine its potential allergenicity with the final aim of analyzing its potential cross-reactivity with Sal k 6 and other allergenic biological sources.…”

Section: Resultsmentioning

confidence: 99%

“…First, the cDNA-encoding sequence for Ole e 14 was subcloned into the pET41b vector. The construct was used to transform BL21(DE3) Escherichia coli cells to produce the recombinant Ole e 14 as a protein fused to a His-tag in the C-terminal end, as it was previously done with Sal k 6 [6]. …”

Section: Resultsmentioning

confidence: 99%

“…Then, solubilization of inclusion bodies with 20 mM Tris-HCl pH 8.0, 0.5 M NaCl, 20 mM imidazole, and 1 mM 2-mercaptoethanol containing 6 M guanidine hydrochloride was performed to get the recombinant protein. Then, Ole e 14 purification was carried out as previously reported [6]. …”

Section: Methodsmentioning

confidence: 99%

“…Recently, pollen-pollen cross-reactivity processes have been reported between PGs from different biological sources using the PG from salsola pollen, Sal k 6, as a model. A high IgE cross-reactivity among Sal k 6 and PGs from different pollens was observed, especially with olive pollen [6]. …”

Section: Introductionmentioning

confidence: 99%

“…The presence of allergenic PGs have been reported in the pollen of several plant families including: (i) the Amaranthaceae family – Sal k 6 from Salsola kali [6]; (ii) the Cupressaceae family – Cha o 2 from Chamaecyparis obtusa , Cry j 2 from Cryptomeria japonica , and Jun a 2 from Juniperus ashei [7-9]; (iii) the Platanaceae family – Pla a 2 from Platanus acerifolia and Pla or 2 from P. orientalis [10, 11], and (iv) the Poaceae family – Pas n 13, Zea m 13, and Phl p 13 from Paspalum notatum , Zea mays , and Phleum pratense , respectively. In spite of the report of several allergenic PGs, the cross-reactivity among PGs has not been deeply analyzed.…”

Section: Introductionmentioning

confidence: 99%

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A Hypoallergenic Polygalacturonase Isoform from Olive Pollen Is Implicated in Pollen-Pollen Cross-Reactivity

Oeo-Santos

Mas

Quiralte

et al. 2018

Int Arch Allergy Immunol

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Background: Cross-reactivity reactions between allergenic polygalacturonases (PGs) from different biological sources, especially foods and pollens from the Oleaceae family, have been described using Salsola kali PG (Sal k 6). No PG from olive pollen has been characterized to date, hampering further knowledge about cross-reactions through PGs. Objectives: The aim of this work was to determine the potential allergenicity of the PG from olive pollen and clarify its role in cross-reactivity. Methods: A cDNA-encoding olive pollen PG sequence was subcloned into the pET41b vector and used to transform BL21(DE3) Escherichia coli cells to produce a His-tag fusion recombinant protein. The allergenic properties of olive pollen PG were determined by immunoblotting and ELISA in comparison to Sal k 6. The cross-reactivity potential of the protein with other pollen sources was analyzed by inhibition immunoassays. Results: The existence of other isoforms of Ole e 14 with different allergenicity was confirmed by proteomics and a meta-analysis of the recently reported olive genome. Sal k 6 showed a higher IgE recognition than Ole e 14 regardless of patient sensitization, suggesting the existence of more allergenic Ole e 14 isoforms in olive pollen. IgG and IgE inhibition assays supported the existence of cross-reactions between them and with other PGs from Oleaceae and Poaceae plant families. Conclusions: A new allergen from olive pollen, Ole e 14, has been identified, produced as a recombinant isoform, and structurally and immunologically characterized. Its role in cross-reactivity has been confirmed and, due to its smaller IgE binding capacity, it could have an important role for therapeutic purposes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Hypoallergenic Polygalacturonase Isoform from Olive Pollen Is Implicated in Pollen-Pollen Cross-Reactivity

Oeo-Santos

Mas

Quiralte

et al. 2018

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

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Peptides and isoflavones in gastrointestinal digests contribute to the anti-inflammatory potential of cooked or germinated desi and kabuli chickpea (Cicer arietinum L.)

et al. 2018

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It is largely unknown how processing affects bioactive potential of chickpea proteins to prevent bowel inflammatory diseases. The aim was to investigate the anti-inflammatory activity of protein concentrates from germinated and cooked chickpeas (GC and CC, respectively) and its relationship with protein and isoflavone composition before and after in vitro gastrointestinal digestion and absorption. Anti-inflammatory activity of GC digests was almost 2-fold higher than CC digests (p < 0.05), which was associated to greater content of peptides, formononetin and biochanin A (p < 0.05). Anti-inflammatory activity of phenolic fraction in digests was 7-fold higher than the protein fraction (p < 0.05). The most active peptide fraction from GC digest (IC = 93 µg/mL) contained a total of 24 peptides derived from legumin and vicilin. In conclusion, this study stands out the potential of germinated chickpea proteins concentrates to exert anti-inflammatory effects in the lower gut which may contribute to the prevention of bowel inflammatory diseases.

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Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy

Tabesh

Fanuel²,

Fazlollahi

et al. 2019

International Immunopharmacology

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A relevant IgE-reactive 28 kDa protein identified from Salsola kali pollen extract by proteomics is a natural degradation product of an integral 47 kDa polygalaturonase

Cited by 13 publications

References 33 publications

A Hypoallergenic Polygalacturonase Isoform from Olive Pollen Is Implicated in Pollen-Pollen Cross-Reactivity

A Hypoallergenic Polygalacturonase Isoform from Olive Pollen Is Implicated in Pollen-Pollen Cross-Reactivity

Peptides and isoflavones in gastrointestinal digests contribute to the anti-inflammatory potential of cooked or germinated desi and kabuli chickpea (Cicer arietinum L.)

Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy

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