A renotropic system in rats.

Preuss, Harry G.; Goldin, Herzl

doi:10.1172/jci108274

Cited by 43 publications

(35 citation statements)

References 15 publications

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“…This resull agrees with the previous observation that the highest renotropic activity in unextracted serum from uninephrectomized rats is obtained when using renal tissue from uninephrectomized animals in the assay [I. 12,13], An increase in the number and/or the af finity of KGF receptors in the remaining kid ney after UNX might explain this finding.…”

Section: Discussionsupporting

confidence: 92%

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Biological Properties of a Renotropic Protein Present in Plasma of Uninephrectomized Rats

Esbrit

Garcı́a-Ocaña²,

García-Cañero³

et al. 1991

Kidney Blood Press Res

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The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4: 334–338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-depend-ently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK₁ cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.

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Section: Discussionsupporting

confidence: 92%

“…These properties of KGF are consistent with those obtained when testing the renotropic activity present in unextracted rat serum or in urine after UNX [12,21,22]. In addition, according to our re sults.…”

Section: Discussionsupporting

confidence: 90%

Biological Properties of a Renotropic Protein Present in Plasma of Uninephrectomized Rats

Esbrit

Garcı́a-Ocaña²,

García-Cañero³

et al. 1991

Kidney Blood Press Res

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show abstract

“…Whole perfused kidney (Shames, Carriere & Berkowitz, 1976), incubated slices (Dicker & Morris, 1980), fragments (Preuss & Goldin, 1976) and primary cultures of kidney epithelium (Lyons, Evan, McLaren & Solomon, 1974) (Pilgrim & Maurer, 1965), which indicates that maximum stimulation for cell division in Fischer rats occurs sometime around 48 h at the latest. The scatter seen in these results might obscure the stimulatory effect of serum from unilaterally nephrectomized rats when less than maximal, i.e.…”

Section: Discussionmentioning

confidence: 95%

Mediation of compensatory renal growth in the rat: cell culture demonstration of a serum factor.

Hansen¹

1982

The Journal of Physiology

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1. The participation ofa circulating growth factor in the mediation ofcompensatory renal growth has been hypothesized but not proven. Theoretical considerations predict that in vitro methods could be profitably used to resolve this question, since such methods would allow dissociation of the growth effects of the postulated factor from the complex physiological changes which accompany compensatory renal growth in vivo.2. A culture system using epithelial cells obtained from the rat kidney is described which is suitable for testing serum from previously unilaterally nephrectomized or sham-operated rats for the presence ofa factor mediating compensatory renal growth. The morphology of the cultured cells is compatible with that of proximal tubule epithelium, this being the cell type stimulated to divide in vivo after unilateral nephrectomy.3. Treatment with serum from rats unilaterally nephrectomized 48 h previously results in consistent increase in uptake of tritiated thymidine by cultures, when compared with control serum from sham-operated rats. Serum from rats unilaterally nephrectomized 18-36 h previously is not consistently stimulatory.4. There are indications that the differential effect of sera from unilaterally nephrectomized and control animals is due to the presence of a stimulatory factor in the former rather than an inhibitor in the latter.5. Use ofthis culture system has confirmed the existence of a serum factor involved in compensatory renal growth.

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“…There have been numerous reports of circulating or humoral factors which alter renal RNA and/or DNA metabolism [6,11,12,17,20], Factors that both stimulate [11,12] and inhibit [20] biochemical growth have been described. Increased RNA and/or DNA accumulation would, thus, result from in creased levels of renal responsiveness to a stimulating factor or from decreased levels of renal responsiveness to an inhibiting fac tor.…”

Section: Discussionmentioning

confidence: 99%

“…and (2) What is the relationship between the biochemical (RNA and DNA) and the physiological (RBF and GFR) changes? While numerous experimentals manipulations and plasma or urine factors have been shown to alter renal RNA, DNA and/or protein metabolism [6,11,12,17,20], the absence of information on the effects of these on renal function precludes definitive conclusions being made with re gard to the overall 'growth' response. Thus resolution of the second question is requisite to elucidation of possible mechanisms.…”

Section: Introductionmentioning

confidence: 99%

RNA and DNA Contents of Kidneys from Rats with Unilateral Ureteral Ligation

Murray¹,

Malvin²,

Northrup³

1979

Kidney Blood Press Res

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Renal RNA and DNA contents, as well as estimates of renal plasma flow and glomerular filtration rate, were studied in albino Sprague-Dawley rats subjected to either unilateral nephrectomy and/or unilateral ureteral ligation. RNA and DNA contents of ligated kidneys were markedly higher than those of the contralateral, unligated kidney 24 and 48 h post ligation. These increases in RNA and DNA content of the ligated kidney occurred in the presence of relative decreases inreal plasma flow and glomerular filtration rate in the ligated kidney. Renal RNA and DNA contents were also increased in the remaining kidney following unilateral nephrectomy. The effect of ureteral ligation and unilateral nephrectomy on renal RNA content was found to be additive. These results are inconsistent with theories stating that changes in renal RNA and DNA content are the result of increased renal plasma flow and glomerular filtration rate. The data support a role for a circulating factor which either stimulates renal RNA and DNA accumulation from the lumental aspect of tubular cells or inhibits renal RNA and DNA accumulation from the peritubular aspect of these cells.

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A renotropic system in rats.

Cited by 43 publications

References 15 publications

Biological Properties of a Renotropic Protein Present in Plasma of Uninephrectomized Rats

Biological Properties of a Renotropic Protein Present in Plasma of Uninephrectomized Rats

Mediation of compensatory renal growth in the rat: cell culture demonstration of a serum factor.

RNA and DNA Contents of Kidneys from Rats with Unilateral Ureteral Ligation

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