A replication-deficient rSV40 mediates liver-directed gene transfer and a long-term amelioration of jaundice in Gunn rats

Sauter, Bernhard; Parashar, Bhupesh; Chowdhury, Namita Roy; Kadakol, Ajit; Ilan, Yaron; Singh, Harminder D.; Milano, Joseph; Strayer, David S.; Chowdhury, Jayanta Roy

doi:10.1053/gast.2000.19577

Cited by 93 publications

(60 citation statements)

References 34 publications

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“…[13][14][15] These vectors have proven to be efficient in therapies for conditions such as the Crigler-Najjar syndrome and a 1 -antitrypsin deficiency. 16,17 In the present work, we show that rSV40 vectors are able to transduce mice liver at least for 7 months and that a rSV40 encoding IGF-I reduces liver cell damage and fibrogenesis and corrects hypogonadism in CCl 4 -treated rats.…”

Section: Introductionmentioning

confidence: 51%

“…[13][14][15][16][17] To evaluate rSV40-mediated liver expression in mice in a more quantitative manner, rSV40 expression of firefly luciferase was visualized with a charge-couple device (CCD) camera. Luciferase expression in the liver was detected for more than 7 months after a single intravenous injection of recombinant SV40-luciferase virus (rSVLuc) (Figure 1a and b).…”

Section: Measurement Of Luciferase Expression In Mice Livers Infectedmentioning

confidence: 99%

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Liver transduction with a simian virus 40 vector encoding insulin-like growth factor I reduces hepatic damage and the development of liver cirrhosis

et al. 2006

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Section: Introductionmentioning

confidence: 51%

Section: Measurement Of Luciferase Expression In Mice Livers Infectedmentioning

confidence: 99%

Liver transduction with a simian virus 40 vector encoding insulin-like growth factor I reduces hepatic damage and the development of liver cirrhosis

et al. 2006

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“…51 SV(BUGT) carries human bilirubin-uridine 5¢-diphosphate-glucuronosyl-transferase, which was used here as negative control vector, with a non-toxic byproduct, as has been reported. 52 …”

Section: Vector Productionmentioning

confidence: 99%

Gene transfer to the rhesus monkey brain using SV40-derived vectors is durable and safe

et al. 2011

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Gene transfer to central nervous system (CNS) has been approached using various vectors. Recombinant SV40-derived vectors (rSV40s) transduce human neurons and microglia effectively in vitro and in rodent brains in vivo, so we tested rSV40s gene transfer to rhesus monkey CNS in vivo, to characterize the distribution, duration and safety of such gene delivery. We used rSV40s carrying HIV-1 RevM10 with a carboxyl-terminal AU1 epitope tag as a marker, and others with the antioxidant enzymes, Cu/Zn superoxide dismutase and glutathione peroxidase. Vectors were injected stereotaxically into the caudate nucleus. Transgene expression was studied at 1 and 6 months by immunostaining serial brain sections. After intraparenchymal administration, numerous transgene-expressing cells were seen, with a longitudinal extent of 20 mm. In neurons and, more rarely, microglial cells, transgene expression remained strong throughout the 6-month study period. Astrocytes and oligodendroglia were not transduced. No evidence of inflammation or tissue damage was observed. SV40-derived vectors may thus be useful for long-term gene expression in the monkey brain and, potentially, in the human brain.

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“…22 Control viruses for these studies have been reported: SV(HBS), SV(Nef/FLAG), and SV(BUGT), which respectively encode hepatitis B surface antigen (HBsAg), HIV-1NL4-3 Nef conjugated to a carboxyl-terminal FLAG epitope tag, and human bilirubin-UDP-glucuronysyltransferase (BUGT). 28,29,34 Production of rSV40 derivative viruses…”

Section: Plasmids and Viral Expression Constructsmentioning

confidence: 99%

Protecting from R5-tropic HIV: individual and combined effectiveness of a hammerhead ribozyme and a single-chain Fv antibody that targets CCR5

Cordelier

Kulkowsky

et al. 2004

Gene Ther

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The CCR5 chemokine receptor is important for most clinical strains of HIV to establish infection. Individuals with naturally occurring polymorphisms in the CCR5 gene who have reduced or absent CCR5 are apparently otherwise healthy, but are resistant to HIV infection. With the goal of reducing CCR5 and protecting CCR5+ cells from R5-tropic HIV, we used Tag-deleted SV40-derived vectors to deliver several anti-CCR5 transgenes: 2C7, a single-chain Fv (SFv) antibody; VCKA1, a hammerhead ribozyme; and two natural CCR5 ligands, MIP-1a and MIP-1b, modified to direct these chemokines, and hence their receptor to the endoplasmic reticulum. These transgenes were delivered using recombinant, Tag-deleted SV40-derived vectors to human CCR5+ cell lines and primary cells: monocyte-derived macrophages and brain microglia. All transgenes except MIP-1a decreased CCR5, as assayed by immunostaining, Northern blotting, and cytofluorimetry (FACS). Individually, all transgenes except MIP-1a protected from low challenge doses of HIV. At higher dose HIV challenges, protection provided by all transgenes diminished, the SFv and the ribozyme being most potent. Vectors carrying these two transgenes were used sequentially to deliver combination anti-CCR5 genetic therapy. This approach gave approximately additive reduction in CCR5, as measured by FACS and protected from higher dose HIV challenges. Reducing cell membrane CCR5 using anti-CCR5 transgenes, alone or in combinations, may therefore provide a degree of protection from R5-tropic strains of HIV.

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A replication-deficient rSV40 mediates liver-directed gene transfer and a long-term amelioration of jaundice in Gunn rats

Cited by 93 publications

References 34 publications

Liver transduction with a simian virus 40 vector encoding insulin-like growth factor I reduces hepatic damage and the development of liver cirrhosis

Liver transduction with a simian virus 40 vector encoding insulin-like growth factor I reduces hepatic damage and the development of liver cirrhosis

Gene transfer to the rhesus monkey brain using SV40-derived vectors is durable and safe

Protecting from R5-tropic HIV: individual and combined effectiveness of a hammerhead ribozyme and a single-chain Fv antibody that targets CCR5

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