A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish

Wakamatsu, Yuma; Kashima, Makoto; Hirata, Hiromi

doi:10.21769/bioprotoc.3712

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…According to Wakamatsu et al, who performed a study with the same swim tunnel, water velocity is linear to the voltage applied with velocity being around 30 cm/sec at 2.5 V and increasing by approximately 13 cm/sec per Volt [24]. Therefore, speed can be estimated from the applied voltage [21]; however, the study mainly focused on the relative improvement of zebrafish swim performance rather than on the absolute speed.…”

Section: Swim Tunnelmentioning

confidence: 99%

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Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish

et al. 2021

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Obesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (Danio rerio) is an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determine whether physical activity is an appropriate tool to examine training effects in zebrafish and to analyse metabolic and transcriptional processes in trained zebrafish. A 2- and 8-week experimental training phase protocol with adult zebrafish in a swim tunnel system was established. We examined zebrafish basic characteristics before and after training such as body weight, body length and maximum speed and considered overfeeding as an additional parameter in the 8-weeks training protocol. Ultimately, the effects of training and overfeeding on blood glucose, muscle core metabolism and liver gene expression using RNA-Seq were investigated. Zebrafish maximum speed was correlated with body length and was significantly increased after 2 weeks of training. Maximum swim speed further increased after 8 weeks of training in both the normal-fed and the overfed groups, but training was found not to be sufficient in preventing weight gain in overfed fish. Metabolome and transcriptome profiling in trained fish exhibited increased blood glucose levels in the short-term and upregulated energy supply pathways as well as response to oxidative stress in the long-term. In conclusion, swim training is a valuable tool to study the effects of physical activity in zebrafish, which is accompanied by metabolic and transcriptional adaptations.

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Section: Swim Tunnelmentioning

confidence: 99%

“…The swim tunnel is based on a propeller, which generates a water flow in a swim tube, forcing the zebrafish to swim against the current. However, the effects of physical performance on physiological parameters and the zebrafish metabolism and transcriptome have rarely been studied so far [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%