A reversed-flow differential flow modulator for comprehensive two-dimensional gas chromatography

Griffith, James F.; Winniford, William L.; Sun, Kai; Edam, Rob; Luong, Jim

doi:10.1016/j.chroma.2011.11.036

Cited by 64 publications

(32 citation statements)

References 16 publications

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“…Dataset 2 includes a reference chromatogram obtained in the previous conditions and a chromatogram of the same sample obtained with a microfluidic modulation system. These GC×GC data were obtained on a Agilent 7890B chromatograph equipped a split/splitless injector, a Griffith [15] type modulation system supplied by the Research Institute for Chromatography (Kortrijk, Belgium) and a FID. The modulation system consists in two Agilent CFT plates (a purged threeway and a two-way splitter) connected to an accumulation capillary.…”

Section: Datasets and Gc×gc Methodsmentioning

confidence: 99%

BARCHAN: Blob Alignment for Robust CHromatographic ANalysis

Duval

Moreaud

Hénon

et al. 2017

Journal of Chromatography A

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Two dimensional gas chromatography (GC×GC) plays a central role into the elucidation of complex samples. The automation of the identification of peak areas is of prime interest to obtain a fast and repeatable analysis of chromatograms. To determine the concentration of compounds or pseudo-compounds, templates of blobs are defined and superimposed on a reference chromatogram. The templates then need to be modified when different chromatograms are recorded. In this study, we present a chromatogram and template alignment method based on peak registration called BARCHAN. Peaks are identified using a robust mathematical morphology tool. The alignment is performed by a probabilistic estimation of a rigid transformation along the first dimension, and a non-rigid transformation in the second dimension, taking into account noise, outliers and missing peaks in a fully automated way. Resulting aligned chromatograms and masks are presented on two datasets. The proposed algorithm proves to be fast and reliable. It significantly reduces the time to results for GC×GC analysis.

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Section: Datasets and Gc×gc Methodsmentioning

confidence: 99%

BARCHAN: Blob Alignment for Robust CHromatographic ANalysis

Duval

Moreaud

Hénon

et al. 2017

Journal of Chromatography A

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“…More recently, a second generation of differential flow modulation was presented [22]; this new configuration, adopts a reverse fill/flush (RFF) injection dynamic instead of the FFF of the first generation. Advantages include: (a) higher efficiency of band re-injection with improved 2 D peak-widths and symmetry, (b) adjustable collection channel volume, (c) better handling of the overloading phenomenon without dramatic loss of separation power and resolution [22,23].…”

Section: Regionmentioning

confidence: 99%

“…Advantages include: (a) higher efficiency of band re-injection with improved 2 D peak-widths and symmetry, (b) adjustable collection channel volume, (c) better handling of the overloading phenomenon without dramatic loss of separation power and resolution [22,23].…”

Section: Regionmentioning

confidence: 99%

Potential of the reversed-inject differential flow modulator for comprehensive two-dimensional gas chromatography in the quantitative profiling and fingerprinting of essential oils of different complexity

Cordero

Rubiolo

Cobelli³

et al. 2015

Journal of Chromatography A

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“…The first commercial FM used fixed volume accumulation loop devices obtained with Capillary Flow Technology (CFT) microfluidic plates. They implement both the forward fill/flush (FFF) injection dynamics described by Seeley et al [7] and the reverse fill/flush (RFF) dynamics connoted by a more efficient band re-injection, improved 2D peak widths and symmetry, and effective handling of collection-channel overloading [8][9][10][11][12]. Commercial RFF modulators are available from Agilent Technologies [13] and by Sep-Solve with the FM named Insight™ [14].…”

Section: Introductionmentioning

confidence: 99%

A step forward in the equivalence between thermal and differential-flow modulated comprehensive two-dimensional gas chromatography methods

Stilo

Gabetti

Bicchi

et al. 2020

Journal of Chromatography A

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Comprehensive two-dimensional gas chromatography (GC×GC) based on flow-modulation (FM) is gaining increasing attention as an alternative to thermal modulation (TM), the recognized GC×GC benchmark, thanks to its lower operational cost and rugged performance. An accessible, rational procedure to perform method translation between the two platforms would be highly valuable to facilitate compatibility and consequently extend the flexibility and applicability of GC×GC. To enable an effective transfer, the methodology needs to ensure preservation of the elution pattern, separation power, and sensitivity. Here, a loop-type thermal modulation system with dual detection (TM-GC×GC-MSD/FID) used for the targeted analysis of allergens in fragrances is selected as reference method. Initially, six different columns configurations are systematically evaluated for the flow-modulated counterpart. The set up providing the most consistent chromatographic separation (20 m x 0.18 mm d c x 0.18 μm d f + 1.8 m x 0.18 mm d c x 0.18 μm d f) is further evaluated to assess its overall performance in terms of sensitivity, linearity, accuracy, and pattern reliability. The experimental results convincingly show that the method translation procedure is effective and allows successful transfer of the target template metadata. Additionally, the FM-GC×GC-MSD/FID system is suitable for challenging applications such as the quantitative profiling of complex fragrance materials.

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A reversed-flow differential flow modulator for comprehensive two-dimensional gas chromatography

Cited by 64 publications

References 16 publications

BARCHAN: Blob Alignment for Robust CHromatographic ANalysis

BARCHAN: Blob Alignment for Robust CHromatographic ANalysis

Potential of the reversed-inject differential flow modulator for comprehensive two-dimensional gas chromatography in the quantitative profiling and fingerprinting of essential oils of different complexity

A step forward in the equivalence between thermal and differential-flow modulated comprehensive two-dimensional gas chromatography methods

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