A reversed phase HPLC-DAD method for the determination of phenolic compounds in plant leaves

Abstract

A simple, fast and efficient HPLC-DAD method was developed for the determination of phenolic compounds in grape leaves. The best separation was achieved in 40 min using gradient elution of methanol, deionized water and acetic acid on Zorbax plus C18 column (4.6 × 100 mm, 3.5 µm) at 25 °C. The phenolic compounds were identified with the help of diode array detector at 280, 320 and 360 nm. The method was precise, accurate, specific and highly reproducible for quantitative and qualitative purposes. The method was used to determine the phenolic composition of grape leaves. A total of 13 phenolic compounds was tentatively identified, namely gallic acid, caffeoylmalic acid, pro-anthocyanin B1, coumaroylhexose-4-O-hexoside, gallic acid derivatives, caffeic acid, quercetin-3-malonylglucoside, quercetin-3-glucoside, kaempferol-3-O-sophorotrioside and kaempferol-3-glucoside. The result demonstrates that this method can be successfully be used for the quality control in the identification of phenolic compounds in grapes and other matrix of plant origin.

Effects of Spinach Leaf Extracts on Quality Characteristics and Phenolic Profile of Sunflower Oil

“…Centrifugation was carried out and again filtered to HPLC vials using Agilent PTFE injection filter (0.45 μm). The residue of the spinach extract was dissolved in methanol–water mixture (60:40, v/v), followed by filtration to HPLC vials …”

Phenolic Profile and Antioxidant Activity of Melon (Cucumis Melo L.) Seeds from Pakistan

“…The binary gradient system consists of solvent A (methanol:acetic acid:deionized water, 10:2:88, v / v / v ) and solvent B (methanol: acetic acid: deionized water, 90:2:8, v / v / v ). The gradient program used was started from 100% A to 85% A at 5 min, and then 50% A at 20 min, 30% A at 25 min, and 100% B from 30 to 40 min [ 16 ]. The elution was achieved at 25 min due to the elution of all compounds present.…”

Phenolic profile and antioxidant potential of wild watercress (Nasturtium officinale L.)

“…The separation was achieved with the help of Agilent Rapid Resolution Zorbax Eclipse plus C18 (4.6 × 100 mm, 3.5 µm) column, which was maintained at 25 °C. The gradient system consists of solvent A (methanol:acetic acid:deionized water, 10:2:88) and solvent B (methanol:acetic acid:deionized water, 90:2:8) (Zeb 2015 ). The gradient program was started with 100 % A at 0 min, 85 % A at 5 min, 50 % A at 20 min, 30 % A at 25 min, and 100 % B from 30 to 40 min.…”