A review on complete silk gene sequencing and de novo assembly of artificial silk

Lu, Wei; Shi, Run; Li, Xue; Ma, Sanyuan; Yang, Daiying; Shang, Deli; Xia, Qingyou

doi:10.1016/j.ijbiomac.2024.130444

Cited by 4 publications

(1 citation statement)

References 120 publications

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“…Nevertheless, the silk produced by these organisms exhibits a distinctive amalgamation of robustness, high tensile strength, and excellent extensibility [ 34 , 73 , 74 , 126 ] surpassing that of other high-performance synthetic fibers. Due to their exceptional biocompatibility and biodegradability, the fibers are especially suitable for biological usage and exhibit extraordinary mechanical properties [ 23 , 36 , 127 ]. For instance, spider silk has been used in fishing lines in previous times, and more recently as microsutures [ 128 , 129 ].…”

Section: Fibrous Proteinsmentioning

confidence: 99%

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Nawaz,

Gu,

Gibbons

et al. 2024

Biomimetics

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The sophisticated, elegant protein-polymers designed by nature can serve as inspiration to redesign and biomanufacture protein-based materials using synthetic biology. Historically, petro-based polymeric materials have dominated industrial activities, consequently transforming our way of living. While this benefits humans, the fabrication and disposal of these materials causes environmental sustainability challenges. Fortunately, protein-based biopolymers can compete with and potentially surpass the performance of petro-based polymers because they can be biologically produced and degraded in an environmentally friendly fashion. This paper reviews four groups of protein-based polymers, including fibrous proteins (collagen, silk fibroin, fibrillin, and keratin), elastomeric proteins (elastin, resilin, and wheat glutenin), adhesive/matrix proteins (spongin and conchiolin), and cyanophycin. We discuss the connection between protein sequence, structure, function, and biomimetic applications. Protein engineering techniques, such as directed evolution and rational design, can be used to improve the functionality of natural protein-based materials. For example, the inclusion of specific protein domains, particularly those observed in structural proteins, such as silk and collagen, enables the creation of novel biomimetic materials with exceptional mechanical properties and adaptability. This review also discusses recent advancements in the production and application of new protein-based materials through the approach of synthetic biology combined biomimetics, providing insight for future research and development of cutting-edge bio-inspired products. Protein-based polymers that utilize nature’s designs as a base, then modified by advancements at the intersection of biology and engineering, may provide mankind with more sustainable products.

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Section: Fibrous Proteinsmentioning

confidence: 99%

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Nawaz,

Gu,

Gibbons

et al. 2024

Biomimetics

View full text Add to dashboard Cite

show abstract

Bioengineered Silk Protein‐Based 3D In Vitro Models for Tissue Engineering and Drug Development: From Silk Matrix Properties to Biomedical Applications

Shuai,

Zheng,

Kundu

et al. 2024

Adv Healthcare Materials

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Abstract3D in vitro model has emerged as a valuable tool for studying tissue development, drug screening, and disease modeling. 3D systems can accurately replicate tissue microstructures and physiological features, mirroring the in vivo microenvironment departing from conventional 2D cell cultures. Various 3D in vitro models utilizing biomacromolecules like collagen and synthetic polymers have been developed to meet diverse research needs and address the complex challenges of contemporary research. Silk proteins, bearing structural and functional similarities to collagen, have been increasingly employed to construct advanced 3D in vitro systems, surpassing the limitations of 2D cultures. This review examines silk proteins' composition, structure, properties, and functions, elucidating their role in 3D in vitro models. Furthermore, recent advances in biomedical applications involving silk‐based organoid models are discussed. In particular, the unique physiological attributes of silk matrix constituents in in vitro tissue constructs are highlighted, providing a meticulous evaluation of their importance. Additionally, it outlines the current research hurdles and complexities while contemplating future avenues, thereby paving the way for developing complex and biomimetic silk protein‐based microtissues.

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Analysis of spider silk in loading-unloading cycles using Raman spectroscopy based on molecular bioinformatics of spidrion

Hong,

Zhang,

et al. 2025

Polymer

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A review on complete silk gene sequencing and de novo assembly of artificial silk

Cited by 4 publications

References 120 publications

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Bioengineered Silk Protein‐Based 3D In Vitro Models for Tissue Engineering and Drug Development: From Silk Matrix Properties to Biomedical Applications

Analysis of spider silk in loading-unloading cycles using Raman spectroscopy based on molecular bioinformatics of spidrion

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