A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis

Albanèse, Véronique; Reißmann, Stefanie; Frydman, Judith

doi:10.1083/jcb.201001054

Cited by 92 publications

(95 citation statements)

References 52 publications

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“…7E; Kaschner et al 2015). Both Jjj1 and RAC have also been implicated in early, nuclear steps of 60S subunit biogenesis, where they may play a role in prerRNA processing, unrelated to Arx1 recycling or nascent chain folding (Albanèse et al 2010). Even though further functional data are required to precisely define the nuclear roles of Jjj1-Ssa and RAC-Ssb, the available data suggest both architectural and functional overlap between these two ribosome-bound chaperone systems.…”

Section: Maturation Of the Central Protuberance And Checkpoint Contromentioning

confidence: 99%

Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy

Greber

2016

RNA

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Eukaryotic ribosomes, the protein-producing factories of the cell, are composed of four ribosomal RNA molecules and roughly 80 proteins. Their biogenesis is a complex process that involves more than 200 biogenesis factors that facilitate the production, modification, and assembly of ribosomal components and the structural transitions along the maturation pathways of the preribosomal particles. Here, I review recent structural and mechanistic insights into the biogenesis of the large ribosomal subunit that were furthered by cryo-electron microscopy of natively purified pre-60S particles and in vitro reconstituted ribosome assembly factor complexes. Combined with biochemical, genetic, and previous structural data, these structures have provided detailed insights into the assembly and maturation of the central protuberance of the 60S subunit, the network of biogenesis factors near the ribosomal tunnel exit, and the functional activation of the large ribosomal subunit during cytoplasmic maturation.

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Section: Maturation Of the Central Protuberance And Checkpoint Contromentioning

confidence: 99%

Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy

Greber

2016

RNA

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“…Later experiments carried out during a study of ribosome biogenesis demonstrated that Zuo1 had both a nuclear and a cytoplasmic localization (62). Deletion of ZUO1 produced a phenotype of slow growth at low temperature and sensitivity to salt, similar to that produced by double deletion of the chaperones Ssb1 and Ssb2.…”

Section: Zuotin a Cochaperone That Activates A Transcription Factormentioning

confidence: 99%

The Expanding Landscape of Moonlighting Proteins in Yeasts

Gancedo

Flores

Gancedo

2016

Microbiol Mol Biol Rev

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SUMMARYMoonlighting proteins are multifunctional proteins that participate in unrelated biological processes and that are not the result of gene fusion. A certain number of these proteins have been characterized in yeasts, and the easy genetic manipulation of these microorganisms has been useful for a thorough analysis of some cases of moonlighting. As the awareness of the moonlighting phenomenon has increased, a growing number of these proteins are being uncovered. In this review, we present a crop of newly identified moonlighting proteins from yeasts and discuss the experimental evidence that qualifies them to be classified as such. The variety of moonlighting functions encompassed by the proteins considered extends from control of transcription to DNA repair or binding to plasminogen. We also discuss several questions pertaining to the moonlighting condition in general. The cases presented show that yeasts are important organisms to be used as tools to understand different aspects of moonlighting proteins.

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“…RAC also binds to ribosomes and is required for the interaction of Ssb with nascent chains (39)(40)(41). RAC/Ssb is connected to diverse cellular functions, including cotranslational folding (38,42,43), translational fidelity (44,45), and transcriptional regulation (46)(47)(48)(49). Recently, we found that RAC/Ssb also plays a role in the quality control of nonstop proteins and of model proteins that possess a bona fide stop codon but contain a C-terminal polylysine segment (termed polylysine proteins) (32).…”

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confidence: 99%

Release Factor eRF3 Mediates Premature Translation Termination on Polylysine-Stalled Ribosomes in Saccharomyces cerevisiae

Chiabudini

Tais

Zhang

et al. 2014

Molecular and Cellular Biology

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bRibosome stalling is an important incident enabling the cellular quality control machinery to detect aberrant mRNA. Saccharomyces cerevisiae Hbs1-Dom34 and Ski7 are homologs of the canonical release factor eRF3-eRF1, which recognize stalled ribosomes, promote ribosome release, and induce the decay of aberrant mRNA. Polyadenylated nonstop mRNA encodes aberrant proteins containing C-terminal polylysine segments which cause ribosome stalling due to electrostatic interaction with the ribosomal exit tunnel. Here we describe a novel mechanism, termed premature translation termination, which releases C-terminally truncated translation products from ribosomes stalled on polylysine segments. Premature termination during polylysine synthesis was abolished when ribosome stalling was prevented due to the absence of the ribosomal protein Asc1. In contrast, premature termination was enhanced, when the general rate of translation elongation was lowered. The unconventional termination event was independent of Hbs1-Dom34 and Ski7, but it was dependent on eRF3. Moreover, premature termination during polylysine synthesis was strongly increased in the absence of the ribosome-bound chaperones ribosome-associated complex (RAC) and Ssb (Ssb1 and Ssb2). On the basis of the data, we suggest a model in which eRF3-eRF1 can catalyze the release of nascent polypeptides even though the ribosomal A-site contains a sense codon when the rate of translation is abnormally low.

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A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis

Abstract: Ribosome-anchored proteins Jjj1 and Zuo1 function together with Hsp70 to mediate ribosome biogenesis (see also the companion paper from Koplin et al. in this issue).

Cited by 92 publications

References 52 publications

Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy

Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy

The Expanding Landscape of Moonlighting Proteins in Yeasts

Release Factor eRF3 Mediates Premature Translation Termination on Polylysine-Stalled Ribosomes in Saccharomyces cerevisiae

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