A Ribosome Assembly Factor Ebp2p, the Yeast Homolog of EBNA1-Binding Protein 2, Is Involved in the Secretory Response

Horigome, Chihiro; Okada, Takafumi; Matsuki, Kana; Mizuta, Keiko

doi:10.1271/bbb.70817

Cited by 17 publications

(25 citation statements)

References 22 publications

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“…The ebp2-14 conditional allele was generated by random-PCR mutagenesis (8). Strain 4795-408 (a gift from Lee Hartwell) was crossed twice with W303α, then subsequently crossed with the Ts − ebp2-14 strain containing Ycp50- EBP2-ADE3 (see below), to generate KM822, the parental strain used to screen for synthetic lethal mutants (Figure 1A).…”

Section: Methodsmentioning

confidence: 99%

“…Signals were visualized by Enhanced Chemiluminescence (Amersham), as instructed by the manufacturer, or otherwise using the colorimetric method (Promega). Mouse anti-myc monoclonal antibodies (9E10; BAbCO) and rabbit anti-Ebp2 polyclonal antibodies (8) were used. Horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (Amersham) were used in 1:1000 dilutions.…”

Section: Methodsmentioning

confidence: 99%

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Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae

Shimoji

Jakovljevic

Tsuchihashi

et al. 2012

Nucleic Acids Research

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The yeast protein Ebp2 is required for early steps in production of 60S ribosomal subunits. To search for cofactors with which Ebp2 functions, or substrates on which it acts, we screened for mutants that were synthetically lethal (sl) with the ebp2-14 mutation. Four different mutant alleles of the 60S ribosomal subunit assembly factor Brx1 were found. To investigate defects of the double mutant, we constructed strains conditional for the ebp2-14 brx1- synthetic lethal phenotype. These ebp2-14 brx1 mutants were defective in processing of 27S pre-rRNA and production of 60S subunits, under conditions where each single mutant was not. Ebp2 and Brx1 exhibit a strong two-hybrid interaction, which is eliminated by some combinations of brx1 and ebp2 mutations. In one such mutant, Ebp2 and Brx1 can still associate with pre-ribosomes, but subunit maturation is perturbed. Depletion of either Ebp2 or Brx1 revealed that Brx1 requires Ebp2 for its stable association with pre-ribosomes, but Ebp2 does not depend on the presence of Brx1 to enter pre-ribosomes. These results suggest that assembly of 60S ribosomal subunits requires cooperation of Ebp2 with Brx1, together with other molecules present in pre-ribosomes, potentially including several found in assembly subcomplexes with Brx1 and Ebp2.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae

Shimoji

Jakovljevic

Tsuchihashi

et al. 2012

Nucleic Acids Research

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“…On the other hand, ebp2-7, even though it is only a weakly temperature-sensitive allele, 21) exhibited a synergistic defect with ard1Á. These results indicate that the synthetic defect seen with ard1Á is specific to ebp2 rather than common to defects in other trans-acting factors of ribosome biogenesis.…”

Section: Resultsmentioning

confidence: 84%

N^α-Acetyltransferase NatA Is Involved in Ribosome Synthesis inSaccharomyces cerevisiae

Wan

Tsuchihashi

Kanda

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…5,10,22,23) This prompted us to examine whether the deletion of GSK-3 kinases resulted in defects in 60S subunit assembly. As both mck1Δ and gsk-3-null strain cells exhibited growth defects at 37°C (Fig.…”

Section: Deletion Of Gsk-3 Affects the Transcriptional Repression Of mentioning

confidence: 99%

Glycogen synthase kinase-3 is involved in regulation of ribosome biogenesis in yeast

Yabuki

Kodama

Katayama

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Secretory defects cause transcriptional repression of both ribosomal proteins and ribosomal RNA genes in Saccharomyces cerevisiae. Rrs1, a trans-acting factor that participates in ribosome biogenesis, is involved in the signaling pathway induced by secretory defects. Here, we found that Rrs1 interacts with two homologs of the glycogen synthase kinase-3 (GSK-3), Rim11, and Mrk1. Rrs1 possesses a repetitive consensus amino acid sequence for phosphorylation by GSK-3, and mutation of this sequence abolished the interaction of Rrs1 with Rim11 and Mrk1. Although this mutation did not affect vegetative cell growth or secretory response, disruption of all four genes encoding GSK-3 homologs, especially Mck1, diminished the transcriptional repression of ribosomal protein genes in response to secretory defects. Among the four GSK-3 kinases, Mck1 appears to be the primary mediator of this response, while the other GSK-3 kinases contribute redundantly.

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A Ribosome Assembly Factor Ebp2p, the Yeast Homolog of EBNA1-Binding Protein 2, Is Involved in the Secretory Response

Cited by 17 publications

References 22 publications

Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae

Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae

N^α-Acetyltransferase NatA Is Involved in Ribosome Synthesis inSaccharomyces cerevisiae

Glycogen synthase kinase-3 is involved in regulation of ribosome biogenesis in yeast

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A Ribosome Assembly Factor Ebp2p, the Yeast Homolog of EBNA1-Binding Protein 2, Is Involved in the Secretory Response

Cited by 17 publications

References 22 publications

Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae

Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae

Nα-Acetyltransferase NatA Is Involved in Ribosome Synthesis inSaccharomyces cerevisiae

Glycogen synthase kinase-3 is involved in regulation of ribosome biogenesis in yeast

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Product

Resources

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N^α-Acetyltransferase NatA Is Involved in Ribosome Synthesis inSaccharomyces cerevisiae