A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO)

Chang, Chi Wei; Chou, Ta Kai; Liu, R.S.; Wang, S.J.; Lin, Wuu-Jyh; Chen, C.H.; Wang, H.E.

doi:10.1016/j.apradiso.2007.01.005

Cited by 25 publications

(13 citation statements)

References 7 publications

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“…The tumor microenviroment is known to be hypoxic, as we confirm by PET imaging (39). Hypoxia controls many phenotypic features of endothelial cells.…”

supporting

confidence: 48%

“…Mice were sacrificed when tumor sizes reached 150 mm 2 . Positron emission tomography (PET) analyses showing tumor hypoxia in CT26 established tumors with the radiotracer fluorine-18-fluoromisonidazole ( 18 FMISO) synthesized as described (39). Mice were injected with 18 F-FMISO (14.9 AE 4.9 MBq in 100 mL) in the tail vein.…”

Section: In Vivo Tumor Growth and Micropet Analysismentioning

confidence: 99%

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Agonist Anti-CD137 mAb Act on Tumor Endothelial Cells to Enhance Recruitment of Activated T Lymphocytes

et al. 2011

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Agonist monoclonal antibodies (mAb) to the immune costimulatory molecule CD137, also known as 4-1BB, are presently in clinical trials for cancer treatment on the basis of their costimulatory effects on primed T cells and perhaps other cells of the immune system. Here we provide evidence that CD137 is selectively expressed on the surface of tumor endothelial cells. Hypoxia upregulated CD137 on murine endothelial cells. Treatment of tumor-bearing immunocompromised Rag À/À mice with agonist CD137 mAb did not elicit any measurable antiangiogenic effects. In contrast, agonist mAb stimulated tumor endothelial cells, increasing cell surface expression of the adhesion molecules intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1, and E-selectin. When adoptively transferred into mice, activated T lymphocytes derived from CD137-deficient animals entered more avidly into tumor tissue after treatment with agonist mAb. This effect could be neutralized with anti-ICAM-1 and anti-VCAM-1 blocking antibodies. Thus, stimulation of CD137 not only enhanced T-cell activation but also augmented their trafficking into malignant tissue, through direct actions on the blood vessels that irrigate the tumor. Our findings identify an additional mechanism of action that can explain the immunotherapeutic effects of agonist CD137 antibodies. Cancer Res; 71(3); 801-11. Ó2011 AACR.

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“…The tumor microenviroment is known to be hypoxic, as we confirm by PET imaging (39). Hypoxia controls many phenotypic features of endothelial cells.…”

supporting

confidence: 48%

Section: In Vivo Tumor Growth and Micropet Analysismentioning

confidence: 99%

Agonist Anti-CD137 mAb Act on Tumor Endothelial Cells to Enhance Recruitment of Activated T Lymphocytes

et al. 2011

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“…Nucleophilic 18 F-fluorination reactions are routinely used to efficiently produce some of the most important 18 (65), [173] and [ 18 F]spiperone (66) [174,175] used in dopamine receptor studies; [60] and [ 18 F]fluoroazomycinarabinofuranoside ([ 18 F]FAZA, 67) [176] and [ 18 F]Fluoromisonidazole ([ 18 F]FMISO, 68) [177,178] for imaging tissue hypoxia. [179,180] Nucleophilic 18 F À is commonly produced by the nuclear reaction 18 O(p,n) 18 18 F À is then eluted from the ion-exchange resin using potassium carbonate in a water/acetonitile solution.…”

Section: Nucleophilic 18 F-substitution Reactionsmentioning

confidence: 99%

Synthesis of ¹¹C, ¹⁸F, ¹⁵O, and ¹³N Radiolabels for Positron Emission Tomography

et al. 2008

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Positron emission tomography (PET) is a powerful and rapidly developing area of molecular imaging that is used to study and visualize human physiology by the detection of positron-emitting radiopharmaceuticals. Information about metabolism, receptor/enzyme function, and biochemical mechanisms in living tissue can be obtained directly from PET experiments. Unlike magnetic resonance imaging (MRI) or computerized tomography (CT), which mainly provide detailed anatomical images, PET can measure chemical changes that occur before macroscopic anatomical signs of a disease are observed. PET is emerging as a revolutionary method for measuring body function and tailoring disease treatment in living subjects. The development of synthetic strategies for the synthesis of new positron-emitting molecules is, however, not trivial. This Review highlights key aspects of the synthesis of PET radiotracers with the short-lived positron-emitting radionuclides (11)C, (18)F, (15)O, and (13)N, with emphasis on the most recent strategies.

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“…[ 18 F]NaF was synthesized following the method described in the literature with slight modification. 43 , 44 In brief, the no-carrier-added 18 F-fluoride was produced by the 18 O(p,n) 18 F nuclear reaction by the bombardment of 95% 18 O-enriched water with a 17-MeV proton beam at 18 mA for 30 min. After irradiation, the solution containing 18 F-fluoride was eluted with a QMA cartridge (Waters, USA) by helium gas pressure, and 18 F ion was trapped in the QMA cartridge, and subsequently washed by distilled water (20 mL).…”

Section: Methodsmentioning

confidence: 99%

“…Preparation and Imaging Analysis of [ 18 F]NaF PET [ 18 F]NaF was synthesized following the method described in the literature with slight modification. 43,44 In brief, the nocarrier-added 18 USA) by helium gas pressure, and 18 F ion was trapped in the QMA cartridge, and subsequently washed by distilled water (20 mL). One milliliter of 0.9% sodium chloride was passed through the QMA cartridge, and [ 18 F]NaF was collected by a tube with 185±25.9 MBq.…”

mentioning

confidence: 99%

Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis

Lu¹,

Chen²,

Ke³

et al. 2021

IJN

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Introduction:Osteoporosis is a result of an imbalance in bone remodeling. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been considered as a potentially promising treatment for osteoporosis. However, the therapeutic effect, genetic alterations, and in vivo behavior of exogenous EVs for osteoporosis in mice models remain poorly understood. Methods: A multiplexed molecular imaging strategy was constructed by micro-positron emission tomography (µPET)/computed tomography (CT), µCT, and optical imaging modality which reflected the osteoblastic activity, microstructure, and in vivo behavior of EVs, respectively. RNA sequencing was used to analyze the cargo of EVs, and the bone tissues of ovariectomized (OVX) mice post EV treatment. Results: The result of [ 18 F]NaF µPET showed an increase in osteoblastic activity in the distal femur of EV-treated mice, and the bone structural parameters derived from µCT were also improved. In terms of in vivo behavior of exogenous EVs, fluorescent dye-labeled EVs could target the distal femur of mice, whereas the uptakes of bone tissues were not significantly different between OVX mice and healthy mice. RNA sequencing demonstrated upregulation of ECMrelated genes, which might associate with the PI3K/AKT signaling pathway, in line with the results of microRNA analysis showing that mir-21, mir-29, mir-221, and let-7a were enriched in Wharton's jelly-MSC-EVs and correlated to the BMP and PI3K/AKT signaling pathways. Conclusion:The therapeutic effect of exogenous WJ-MSC-EVs in the treatment of osteoporosis was successfully assessed by a multiplexed molecular imaging strategy. The RNA sequencing demonstrated the possible molecular targets in the regulation of bone remodeling. The results highlight the novelty of diagnostic and therapeutic strategies of EV-based treatment for osteoporosis.

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A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO)

Cited by 25 publications

References 7 publications

Agonist Anti-CD137 mAb Act on Tumor Endothelial Cells to Enhance Recruitment of Activated T Lymphocytes

Agonist Anti-CD137 mAb Act on Tumor Endothelial Cells to Enhance Recruitment of Activated T Lymphocytes

Synthesis of ¹¹C, ¹⁸F, ¹⁵O, and ¹³N Radiolabels for Positron Emission Tomography

Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis

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A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO)

Cited by 25 publications

References 7 publications

Agonist Anti-CD137 mAb Act on Tumor Endothelial Cells to Enhance Recruitment of Activated T Lymphocytes

Agonist Anti-CD137 mAb Act on Tumor Endothelial Cells to Enhance Recruitment of Activated T Lymphocytes

Synthesis of 11C, 18F, 15O, and 13N Radiolabels for Positron Emission Tomography

Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis

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Synthesis of ¹¹C, ¹⁸F, ¹⁵O, and ¹³N Radiolabels for Positron Emission Tomography