A robust capillary liquid chromatography/tandem mass spectrometry method for quantitation of neuromodulatory endocannabinoids

Abstract

The microscale method is a sensitive, economical and robust alternative to the larger scale LC/MS methods currently implemented for quantitation of AEA and 2-AG.

Endocannabinoid regulation of homeostatic feeding and stress‐induced alterations in food intake in male rats

“…Drug naïve rats were killed after stress or homecage food restriction by rapid decapitation, and tissues were collected as described above. Lipid extractions were carried out as previously described (Qi et al ., ). Briefly, frozen brain and gut tissue was weighed and then manually homogenized (with a glass rod) in borosilicate glass culture tubes containing 2 mL of acetonitrile with 5 nmol of d8‐2‐AG, 5 pmol of d8‐AEA, 40 pmol d4‐PEA and 40 pmol d4‐OEA.…”

“…After complete drying, the samples were re‐suspended in 200 μL of acetonitrile and stored at −80°C until analysis by LC–MS. Analysis by MS was performed exactly as previously described (Qi et al ., ).…”

Deficient adolescent social behavior following early-life inflammation is ameliorated by augmentation of anandamide signaling

“…To determine the impact of FAAH inhibition on social behavior in saline and LPS administered animals, we used a previously validated method of oral administration of the FAAH inhibitor PF-04457845, which results in significant and selective elevations in AEA, but not 2-AG in central tissue 4h following administration (Qi et al, 2015). Specifically, on P35 (+/− 1) animals were habituated to handling and the consumption of a 100mg of peanut butter pellet for 4 consecutive days.…”

“…The following day, test subjects were given a peanut butter pellet containing 1 mg/kg of the selective FAAH inhibitor PF-04457845 or a control peanut butter pellet with no drug. Consistent with our previous work demonstrating elevations in central AEA levels (Qi et al, 2015), we chose a testing window of 4–6h after oral administration of PF-04457845. At that time point, animals were placed in the arena where they encountered a novel rat, matched for treatment, age and sex.…”

Desenvolvimento de fases extratoras seletivas para técnicas de microextração para a determinação de fármacos e endocanabinóide em amostras de plasma por LC-MS/MS

“…O sistema endocanabinóide compreende uma família de moléculas lipídicas neuromoduladoras conhecidas como "endocanabinóides", seus receptores conhecidos como "receptores canabinóides" (CB) e um conjunto de enzimas que catalisam a síntese e a degradação destas moléculas (QI et al, 2015 A determinação de endocanabinóides, em diferentes matrizes biológicas, tem sido alvo de muitos trabalhos (Tabela II.11). As concentrações encontradas variam, segundo a matriz analisada.…”