2023
DOI: 10.1101/2023.10.09.561342
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A robust life-or-death selection platform for enzyme evolution

Suzanne C. Jansen,
Clemens Mayer

Abstract: Life-or-death selections evaluate the fitness of individual organisms on a population level. In enzyme engineering, such growth selections allow the rapid and straightforward identification of highly efficient biocatalysts from extensive libraries. However, selection-based improvement of (industrially-relevant) biocatalysts is challenging, as they require highly dependable strategies that artificially link their activities to host survival. Here, we showcase a robust and scalable life-or-death selection platfo… Show more

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