A robust luminescent assay for screening alkyladenine DNA glycosylase inhibitors to overcome DNA repair and temozolomide drug resistance

Anti-tumor effect and mechanism of the total biflavonoid extract from S doederleinii on human cervical cancer cells in vitro and in vivo

“…Mitochondrial membrane potential evaluation was performed according to previous reports [ 21 , [23] , [24] , [25] ]. HeLa cells were cultured until they reached a fusion of 70 %, after which they were digested with trypsin and adjusted to a cell density of approximately 1 × 10 5 /mL with 10 % RPMI-1640 medium.…”

Long non-coding RNA LIP interacts with PARP-1 influencing the efficiency of base excision repair

“…To investigate whether the transcriptional upregulation of LIP is related to a specific DNA lesion type, the TMZ was selected to induce DNA lesions, which caused DNA damage primarily eliminated by the BER pathway. According to publications, over 80% of DNA damage induced TMZ consists of N7-methyl guanine and N3-methyl adenine, both repaired through the BER pathway [ 23 , 24 ]. Remarkably, treatment with 1 mM TMZ resulted in a significant upregulation of LIP in HeLa cells ( Fig.…”

Temozolomide, Procarbazine and Nitrosoureas in the Therapy of Malignant Gliomas: Update of Mechanisms, Drug Resistance and Therapeutic Implications

“…High nuclear MPG levels (associated with epigenetic silencing of HTATIP2, a negative regulator of cytoplasmicnuclear translocation) appear to be associated with resistance to methylating agents [154]. Drug screening revealed Sunitinib to be effective in inhibiting MPG, which restored TMZ sensitivity [155], suggesting repurposing the drug in glioma therapy. Overall, in MGMT proficient-tumors, imbalances in BER may contribute to drug resistance as well as sensitivity.…”