A role for nitrite in the production of nitrous oxide in the lower euphotic zone of the oligotrophic North Pacific Ocean

Wilson, Samuel T.; Valle, Daniela A. del; Segura-Noguera, Mariona; Karl, David M.

doi:10.1016/j.dsr.2013.11.008

Cited by 31 publications

(35 citation statements)

References 44 publications

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“…Thus, since the PRT Marinosulfonomonas SAG contains genes associated with N 2 O reduction (e.g., nosZ), it is possible that it employs N 2 O in the oxidation of organic matter (Table 2). It has been proposed that nitrification leads to N 2 O production in sinking particles (60). The PRT Marinosulfonomonas, which was recovered from an amphipod, may make use of the N 2 O produced from incomplete denitrification processes associated with the amphipod microbiome, sediments, or particles ingested by amphipods.…”

Section: Resultsmentioning

confidence: 99%

Single Cells within the Puerto Rico Trench Suggest Hadal Adaptation of Microbial Lineages

León-Zayas

Novotny

Podell

et al. 2015

Appl Environ Microbiol

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f Hadal ecosystems are found at a depth of 6,000 m below sea level and below, occupying less than 1% of the total area of the ocean. The microbial communities and metabolic potential in these ecosystems are largely uncharacterized. Here, we present four single amplified genomes (SAGs) obtained from 8,219 m below the sea surface within the hadal ecosystem of the Puerto Rico Trench (PRT). These SAGs are derived from members of deep-sea clades, including the Thaumarchaeota and SAR11 clade, and two are related to previously isolated piezophilic (high-pressure-adapted) microorganisms. In order to identify genes that might play a role in adaptation to deep-sea environments, comparative analyses were performed with genomes from closely related shallow-water microbes. The archaeal SAG possesses genes associated with mixotrophy, including lipoylation and the glycine cleavage pathway. The SAR11 SAG encodes glycolytic enzymes previously reported to be missing from this abundant and cosmopolitan group. The other SAGs, which are related to piezophilic isolates, possess genes that may supplement energy demands through the oxidation of hydrogen or the reduction of nitrous oxide. We found evidence for potential trench-specific gene distributions, as several SAG genes were observed only in a PRT metagenome and not in shallower deep-sea metagenomes. These results illustrate new ecotype features that might perform important roles in the adaptation of microorganisms to life in hadal environments.

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Section: Resultsmentioning

confidence: 99%

Single Cells within the Puerto Rico Trench Suggest Hadal Adaptation of Microbial Lineages

León-Zayas

Novotny

Podell

et al. 2015

Appl Environ Microbiol

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“…New models of archaeal ammonia oxidation indicate a key role for NO produced by nitrite reductase encoded by the gene AnirK 22 providing a clear link to the production of N 2 O, possibly via hybrid N 2 O formation22232425. A role for AOA-mediated N 2 O production has been suggested in the oceans, with process, natural abundance isotope values and molecular data supporting this idea12202627. There has, however, been no formal experimental characterization of N 2 O production at oxygen concentrations representative of the margins of an OMZ (<62.5 μmol O 2 l −1 ) where N 2 O accumulates228 and/or the abundance of AOA (or any other candidate organisms) in representative samples of the ocean13.…”

mentioning

confidence: 99%

Nitrous oxide as a function of oxygen and archaeal gene abundance in the North Pacific

et al. 2016

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Oceanic oxygen minimum zones are strong sources of the potent greenhouse gas N2O but its microbial source is unclear. We characterized an exponential response in N2O production to decreasing oxygen between 1 and 30 μmol O2 l−1 within and below the oxycline using 15NO2−, a relationship that held along a 550 km offshore transect in the North Pacific. Differences in the overall magnitude of N2O production were accounted for by archaeal functional gene abundance. A one-dimensional (1D) model, parameterized with our experimentally derived exponential terms, accurately reproduces N2O profiles in the top 350 m of water column and, together with a strong 45N2O signature indicated neither canonical nor nitrifier–denitrification production while statistical modelling supported production by archaea, possibly via hybrid N2O formation. Further, with just archaeal N2O production, we could balance high-resolution estimates of sea-to-air N2O exchange. Hence, a significant source of N2O, previously described as leakage from bacterial ammonium oxidation, is better described by low-oxygen archaeal production at the oxygen minimum zone's margins.

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“…O 2 concentrations in experiments were determined by membrane inlet mass spectrometry as described in Wilson et al (2014). Water column O 2 profiles were obtained using an O 2 sensor (ISUS, version 3, Satlantic), calibrated against O 2 concentrations determined from Winkler titrations of discrete samples.…”

Section: Analytical Measurementsmentioning

confidence: 99%

Aerobic production of methane from dissolved water-column methylphosphonate and sinking particles in the North Pacific Subtropical Gyre

Valle¹,

Karl²

2014

Aquat. Microb. Ecol.

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A role for nitrite in the production of nitrous oxide in the lower euphotic zone of the oligotrophic North Pacific Ocean

Cited by 31 publications

References 44 publications

Single Cells within the Puerto Rico Trench Suggest Hadal Adaptation of Microbial Lineages

Single Cells within the Puerto Rico Trench Suggest Hadal Adaptation of Microbial Lineages

Nitrous oxide as a function of oxygen and archaeal gene abundance in the North Pacific

Aerobic production of methane from dissolved water-column methylphosphonate and sinking particles in the North Pacific Subtropical Gyre

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