A role for the RNA-binding protein MOS2 in microRNA maturation in Arabidopsis

Wu, Xueying; Shi, Yupeng; Li, Jingrui; Xu, Le; Fang, Yuda; Li, Xin; Qi, Yijun

doi:10.1038/cr.2013.23

Cited by 93 publications

(86 citation statements)

References 52 publications

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“…The interaction of MAC3A/B with the DCL1 complex also prompted us to test the effect of 299 mac3a mac3b on the formation of the D-body. We crossed a HYL1-YFP transgenic line, which 300 has been used as a reporter for the D-body Wu et al, 2013;Qiao et al, 2015), 301 into mac3a mac3b and examined the percentage of cells containing D-bodies in the root tips and 302 elongation region. As previously reported (Wu et al, 2013), the HYL1-containing D-bodies 303 existed in most cells (~ 84%, Figure 6A, 6B and Supplemental Figure 6A Because MAC3A has considerable sequence difference from MAC3B, we tested if it is a 318 ubiquitin E3 ligase using MBP-MAC3A (Supplemental Figure 5A).…”

Section: Mac3a Binds Pri-mirnas In Vivo 274mentioning

confidence: 99%

MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis

Liu

Zhou

et al. 2018

Plant Cell

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Section: Mac3a Binds Pri-mirnas In Vivo 274mentioning

confidence: 99%

MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis

Liu

Zhou

et al. 2018

Plant Cell

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“…Four other proteins, DAWDLE (DDL; an RNA binding protein), Cap-binding protein 20, Cap-binding protein 80, and NOT2, which are associated with the DCL1 complex (8,(20)(21)(22), also function in miRNA biogenesis. Recent studies also reveal that the correct localization of DCL1 requires NOT2 and MODIFIER OF SNC1, 2 (an RNA binding protein) (8,23). In addition, the accumulation of a subset of miRNAs requires a proline-rich protein named SICKLE (24).…”

mentioning

confidence: 99%

CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts

Zhang

Xie

Ren

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

140

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CDC5 is a MYB-related protein that exists in plants, animals, and fungi. In Arabidopsis, CDC5 regulates both growth and immunity through unknown mechanisms. Here, we show that CDC5 from Arabidopsis positively regulates the accumulation of microRNAs (miRNAs), which control many biological processes including development and adaptations to environments in plants. CDC5 interacts with both the promoters of genes encoding miRNAs (MIR) and the DNA-dependent RNA polymerase II. As a consequence, lack of CDC5 reduces the occupancy of polymerase II at MIR promoters, as well as MIR promoter activities. In addition, CDC5 is associated with the DICER-LIKE1 complex, which generates miRNAs from their primary transcripts and is required for efficient miRNA production. These results suggest that CDC5 may have dual roles in miRNA biogenesis: functioning as a positive transcription factor of MIR and/or acting as a component of the DICER-LIKE1 complex to enhance primary miRNA processing.M icroRNAs (miRNAs) and small interfering RNAs (siRNAs) are ∼22-nucleotide (nt) noncoding RNAs that regulate various biological processes including development, metabolism, and immunity in plants and animals (1-3). miRNAs are generated from primary miRNA transcripts (pri-miRNAs) containing stem-loop structure, whereas siRNAs are derived from long, perfect, doublestranded RNAs (dsRNAs) (1-3). They are associated with members of the Argonaute protein family to repress gene expression at posttranscriptional and/or transcriptional levels (1-3). In addition to miRNAs, plants encode two major classes of siRNAs: siRNAs derived from repeated DNAs (ra-siRNAs) and transacting siRNAs (ta-siRNAs) (4-6).Studies in Arabidopsis have established the framework of miRNA biogenesis in plants (1-3). In Arabidopsis, pri-miRNAs are primarily transcribed by DNA-dependent RNA polymerase II (Pol II), with assistance from the mediator complex and the transcription factor Negative on TATA less2 (NOT2) (7,8). After transcription, pri-miRNAs are processed by an RNase III enzyme called DICER-LIKE1 (DCL1) to miRNA precursors and then to mature miRNAs (9, 10). The efficient processing of pri-miRNA requires SERRATE (SE; a zinc finger protein), TOUGH (an RNA-binding protein), and a dephosphorylated HYPONASTIC LEAVES1 (HYL1; a double-stranded RNA binding protein) that form a complex with DCL1 (11-18). SE and HYL1 also promote the processing accuracy of pri-miRNAs (19). Four other proteins, DAWDLE (DDL; an RNA binding protein), Cap-binding protein 20, Cap-binding protein 80, and NOT2, which are associated with the DCL1 complex (8,(20)(21)(22), also function in miRNA biogenesis. Recent studies also reveal that the correct localization of DCL1 requires NOT2 and MODIFIER OF SNC1, 2 (an RNA binding protein) (8,23). In addition, the accumulation of a subset of miRNAs requires a proline-rich protein named SICKLE (24).The cell division cycle 5 (CDC5) protein is a conserved protein in animals, plants, and fungi (25). It was first isolated from Schizosaccharomyces pombe as a cell cycle regulat...

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“…7 Several miRNAs have been shown to be involved in antibacterial resistance. 8,9 The function of MOS2 in defense may be partly explained by its role in miRNA maturation.…”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

“…Recently it was further shown that MOS2 is involved in micro RNA (miRNA) maturation by binding to long primary miRNA (pri-miRNA) transcripts and facilitating their recruitment to HYL1, a protein that assists in the production of miRNAs through the cleavage of pri-miRNA. 7 However, how miRNA maturation affects snc1-mediated autoimmunty is unclear.…”

mentioning

confidence: 99%

“…It has been suggested that the RNA-binding activity of MOS2 may be involved in the recruitment of pri-miRNA molecules to HYL1 and other proteins responsible for processing into miRNAs. 7 It is possible that MOS2 also binds mRNA and recruits it to the MAC, which further facilitates proper splicing of SNC1 transcript. 10 Members of the MAC and other pre-mRNA splicing factors have also been implicated in control of RNA-directed DNA methylation, suggesting that splicing-related proteins may play roles in diverse cellular processes.…”

mentioning

confidence: 99%

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MOS2 has redundant function with its homolog MOS2H and is required for proper splicing ofSNC1

Copeland

Qi³

et al. 2013

Plant Signaling & Behavior

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A role for the RNA-binding protein MOS2 in microRNA maturation in Arabidopsis

Cited by 93 publications

References 52 publications

MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis

MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis

CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts

MOS2 has redundant function with its homolog MOS2H and is required for proper splicing ofSNC1

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