A role for tricellulin in the regulation of gill epithelium permeability

Kolosov, Dennis; Kelly, Scott P.

doi:10.1152/ajpregu.00086.2013

Cited by 23 publications

(30 citation statements)

References 60 publications

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“…This group further reported that the increased macromolecule permeation at the tTJs of intestinal epithelial cells by the treatment of caprate, a 10-carbon medium chain fatty acid clinically employed as a drug absorption-enhancing agent, correlated with the loss of TC localization of tricellulin 24 . Tricellulin removal by caprate was also observed in the fish gill epithelium 25 . It would be interesting to determine whether loss of tricellulin at TCs by caprate treatment is accompanied by abnormal localization of angulins.…”

Section: Contribution Of Ttjs To Epithelial Barrier Functionmentioning

confidence: 99%

Molecular organization of tricellular tight junctions

et al. 2014

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When the apicolateral border of epithelial cells is compared with a polygon, its sides correspond to the apical junctional complex, where cell adhesion molecules assemble from the plasma membranes of two adjacent cells. On the other hand, its vertices correspond to tricellular contacts, where the corners of three cells meet. Vertebrate tricellular contacts have specialized structures of tight junctions, termed tricellular tight junctions (tTJs). tTJs were identified by electron microscopic observations more than 40 years ago, but have been largely forgotten in epithelial cell biology since then. The identification of tricellulin and angulin family proteins as tTJ-associated membrane proteins has enabled us to study tTJs in terms of not only the paracellular barrier function but also unknown characteristics of epithelial cell corners via molecular biological approaches.

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Section: Contribution Of Ttjs To Epithelial Barrier Functionmentioning

confidence: 99%

Molecular organization of tricellular tight junctions

et al. 2014

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“…Goldfish -SSI Effect of ion poor water on epithelium permeability, and expression of tight junction proteins and use of siRNA Kolosov and Kelly, 2013 Rainbow trout -SSI Properties of the tight junction protein tricellulin …”

Section: The Primary Gill Cell Culturementioning

confidence: 99%

“…Adapted with kind permission from The Journal of Experimental Biology (Fletcher et al, 2000). , 1997;Fletcher et al, 2000;Carlsson and Pärt, 2001;Smith et al, 2001;Kelly and Wood, 2001a;Kelly and Wood, 2001c;Kelly and Wood, 2002a;Shahsavarani et al, 2006;Jönsson et al, 2006;Chasiotis et al, 2010;Sandbichler et al, 2011a;Sandbichler et al, 2011b;Kelly and Chasiotis, 2011;Kolosov and Kelly, 2013 Depending on the quality of the data available, e.g. whether the data are acute or chronic and whether the species sensitivity distribution (SSD) plots are representative of a large range of species and taxa, a further assessment (or safety) factor (e.g.…”

Section: Toxicity Testsmentioning

confidence: 99%

“…The cultured gill epithelium expresses a number of tight junction proteins, such as the claudins and ZO-1, that enable the cells to forms a tight epithelium with a transepithelial electrical resistance in excess of 20 kΩ (e.g. Chasiotis et al, 2010; Chasiotis and Kelly, 2011a;Kolosov and Kelly, 2013). The claudins are a large superfamily of proteins that are anchored in the membrane and facilitate paracellular transport.…”

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Gill cell culture systems as models for aquatic environmental monitoring

Bury

Schnell

Högstrand

2014

Journal of Experimental Biology

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A vast number of chemicals require environmental safety assessments for market authorisation. To ensure acceptable water quality, effluents and natural waters are monitored for their potential harmful effects. Tests for market authorisation and environmental monitoring usually involve the use of large numbers of organisms and, for ethical, cost and logistic reasons, there is a drive to develop alternative methods that can predict toxicity to fish without the need to expose any animals. There is therefore a great interest in the potential to use cultured fish cells in chemical toxicity testing. This review summarises the advances made in the area and focuses in particular on a system of cultured fish gill cells grown into an epithelium that permits direct treatment with water samples. KEY WORDS: FIGCS, Biomonitoring, Environmental risk assessment, Fish, In vitro, Toxicology IntroductionThe Industrial Revolution caused a rapid rise in the use of raw materials and urbanisation as the populace moved to the cities for employment. Since this time, there has been a continuous increase in living standards that to a large part has been fuelled by innovations within the chemical and pharmaceutical industry. Life expectancy has increased as a result of great advances in medical practices and effective drugs against many fatal diseases. The increase in life expectancy has seen the population of the world grow, reaching 7 billion in 2012, and to feed this population there have been great advances in agricultural productivity partly via the development of pesticides and nitrate/phosphate-based fertilisers. These activities have altered the geochemical cycling of elements, increasing or decreasing concentrations in earth system compartments and increasing global distribution (Doney, 2010). Anthropogenic activities have left a cumulative and lasting impression on the biosphere -so much so that geologists have termed the current epoch the anthropocene (Zalasiewicz et al., 2010).The increase in agricultural and industrial production and consumption of raw materials has resulted in the creation of vast amounts of waste that enters the aquatic ecosystem. An acknowledgement of the decline in environmental quality due to contaminants has led to the development of environmental quality standards (EQS) in many countries, and to assess whether these standards are being adhered to, many jurisdictions also have a programme of waste water effluent testing (WET) and/or biomonitoring. The EQS are derived from toxicity tests that use numerous organisms per compound, and in order to set standards several species are tested. In the USA ~3 million fish are used in WET procedures (see Tanneberger et al., 2013). There is a move towards reducing the number of animals used in research and toxicology studies and there are a number of international initiatives aimed at investigating the 3Rs -reduction, replacement and refinement -in animal research (for example, see http://www.nc3rs.org.uk/). Within the context of the need to determine EQS fo...

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“…In aquatic vertebrates such as fishes, the tricellular tight junction (TJ) protein tricellulin has been proposed to play a role in maintaining the barrier properties of osmoregulatory organs such as the gill (Kolosov and Kelly, 2013). Therefore, it seems reasonable to consider that in the functionally analogous occluding junction of an aquatic arthropod facing the same physiological problems as that of an aquatic vertebrate, a tricellular SJ protein may also contribute to salt and water balance.…”

Section: Introductionmentioning

confidence: 99%

Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

Jonusaite

Kelly

Donini

2017

Journal of Experimental Biology

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Septate junctions (SJs) regulate paracellular permeability across invertebrate epithelia. However, little is known about the function of SJ proteins in aquatic invertebrates. In this study, a role for the transmembrane SJ protein gliotactin (Gli) in the osmoregulatory strategies of larval mosquito (Aedes aegypti) was examined. Differences in gli transcript abundance were observed between the midgut, Malpighian tubules, hindgut and anal papillae of A. aegypti, which are epithelia that participate in larval mosquito osmoregulation. Western blotting of Gli revealed its presence in monomer, putative dimer and alternatively processed protein forms in different larval mosquito organs. Gli localized to the entire SJ domain between midgut epithelial cells and showed a discontinuous localization along the plasma membranes of epithelial cells of the rectum as well as the syncytial anal papillae epithelium. In the Malpighian tubules, Gli immunolocalization was confined to SJs between the stellate and principal cells. Rearing larvae in 30% seawater caused an increase in Gli protein abundance in the anterior midgut, Malpighian tubules and hindgut. Transcriptional knockdown of gli using dsRNA reduced Gli protein abundance in the midgut and increased the flux rate of the paracellular permeability marker, polyethylene glycol (molecular weight 400 Da; PEG-400). Data suggest that in larval A. aegypti, Gli participates in the maintenance of salt and water balance and that one role for Gli is to participate in the regulation of paracellular permeability across the midgut of A. aegypti in response to changes in environmental salinity.

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A role for tricellulin in the regulation of gill epithelium permeability

Cited by 23 publications

References 60 publications

Molecular organization of tricellular tight junctions

Molecular organization of tricellular tight junctions

Gill cell culture systems as models for aquatic environmental monitoring

Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

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