A Role for WDR5 in Integrating Threonine 11 Phosphorylation to Lysine 4 Methylation on Histone H3 during Androgen Signaling and in Prostate Cancer

Abstract

In the article by Kim et al., we the authors made some errors during final preparation of a few figures (mostly on proper marking of lanes). We have corrected these errors as follows:Under Figures 1A, 1D, and S1F, we failed to indicate that the adjacent lanes are non-continuous but from the same gel. Lines have now been added to indicate this.In Figure 1C and its corresponding Figure S1F, under Coomassie blue, we inadvertently ran and presented four or three, respectively, lanes of purified GST-WDR5 input. We can see how this could be confusing, so now we are only showing one lane. As also noted in the original paper, input lanes are identical in Figures 1C and S1F. These corrections do not alter the conclusions of the original paper; however, we apologize for any inconvenience or confusion that this may have caused. Figure 1. Revised Panels from Original Figure 1 and Figure S1 Molecular Cell 58, 557, May 7, 2015 ª2015 Elsevier Inc. 557

In Vivo Functional Platform Targeting Patient-Derived Xenografts Identifies WDR5-Myc Association as a Critical Determinant of Pancreatic Cancer

“…Recently, WDR5 upregulation was detected in prostate and bladder cancers, where it was also found to be critical for cancer cell proliferation (Kim et al, 2014;Chen et al, 2015). We first confirmed deregulated expression of WDR5 in human PDAC compared to normal control pancreas ( Figure 3A).…”

KAT8 Regulates Androgen Signaling in Prostate Cancer Cells

“…All culture media except for RWPE-1cells were supplemented with 10% fetal bovine serum (FBS). For androgen treatment, cells were hormone-starved for 2 days in media containing 10% charcoal stripped FBS and treated with androgen as previously described (10). LNCaP cells were hormone-starved for 2 days and treated with 10 nM R1881 or 1 nM DHT for 16 hours (for expression assay) or for 3 hours (for ChIP assays).…”

“…After overnight transfection, fresh DMEM was added to cells. WDR5, and PKN1 pSuper.Retro-.Puro retroviral shRNA expression vectors have been described previously (10). VSV-G-pseudotyped lentivirus or retrovirus was used to spin-infect cells as previously described (23).…”

“…Whole-cell extracts were prepared from cells expressing indicated shRNA(s) after 3 days posttransduction and immunoblotted as previously described (10). Total RNA was prepared from shRNA-expressing cells after 6 days posttransduction and analyzed by quantitative RT-PCR as previously described (10).…”

“…Previous studies have shown that the kinase PKN1 (previously known as PRK1) is recruited to AR target genes, leading to hyperphosphorylation of histone H3 at threonine 11 (H3T11P) upon stimulation of cells with androgen to activate gene expression (9). Additionally, we have shown that H3T11P associates with the WD repeat-containing protein 5 (WDR5), leading to recruitment of SET1/MLL histone methyltransferase (HMTase) complex to AR target genes in androgen stimulated LNCaP cells and an integrated PKN1-H3T11P-WDR5/MLL pathway is important for AR target gene activation and regulation of prostate cancer cell growth (10).…”