A Ruthenium(II) Complex Supported by Trithiacyclononane and Aromatic Diimine Ligand as Luminescent Switch-On Probe for Biomolecule Detection and Protein Staining

Wong, Chun‐Yuen; Chung, Lai‐Hon; Lin, Sheng; Chan, Daniel Shiu‐Hin; Leung, Chung‐Hang; Ma, Dik‐Lung

doi:10.1038/srep07136

Cited by 26 publications

(12 citation statements)

References 52 publications

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“…From Figure 9 b and Table 4 , it can be further seen that G' shows a stronger increase than G'' with increasing MDI content and thus being significantly higher than G'' in case of the highly crosslinked compounds. These effects have been reported by many researchers for different polymers [ 30 , 31 , 35 , 36 ] and shows that elastic response is predominant in highly crosslinked compounds.…”

Section: Resultssupporting

confidence: 72%

“…The characteristic absorption band of the carbonyl-urethane group for isocyanate crosslinked CA is also observed by other researchers [ 28 , 29 ]. Furthermore, Xing et al [ 30 ] described the characteristic N–H deformation around 1530 cm −1 in gelatin hydrogels. However, additional analytical methods have to be used (e.g., solid-state nuclear magnetic resonance (NMR) spectroscopy) to obtain further information on the complex network structure of the crosslinked compounds and in case of CA/TEC (85/15) to elucidate whether a certain amount of MDI is grafted onto TEC or not.…”

Section: Resultsmentioning

confidence: 99%

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Study of Reactive Melt Processing Behavior of Externally Plasticized Cellulose Acetate in Presence of Isocyanate

et al. 2014

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Two types of externally plasticized cellulose acetate (CA) were chemically modified using 4,4'-methylene diphenyl diisocyanate (MDI) as crosslinking agent. Crosslinking was performed in the molten state by means of melt mixing in an internal mixer. The viscoelastic properties of the non-crosslinked, externally plasticized CA show typical temperature dependence, similar to conventional thermoplastics. A strong increase in storage modulus is observed with increasing crosslink density indicating that the crosslinked compounds exhibit predominately elastic response. The complex viscosity also increases considerably with increasing crosslink density and does not reach the typical Newtonian plateau at low radial frequencies any more. The viscoelastic properties correlate well with the data recorded online during reactive melt processing in the internal mixer. In comparison to the non-crosslinked CA, the crosslinked compounds show higher glass transition temperature, higher VICAT softening temperatures, improved thermal stability and lower plasticizer evaporation at evaluated temperatures.

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Study of Reactive Melt Processing Behavior of Externally Plasticized Cellulose Acetate in Presence of Isocyanate

et al. 2014

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“…None of the products tested demonstrated >50% efficacy against the resistant strain of bed bugs. This is not surprising in light of recent studies demonstrating that insecticide resistance is increasing in field populations of bed bugs, even to the successful dual action products, such as Temprid ® and Transport ® [ 24 , 25 , 26 ]. However, Cimexa ® killed approximately half of resistant bugs on the upholstery fabric, while Temprid ® killed 32% on ceramic tiles ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 97%

Long-Term Efficacy of Various Natural or “Green” Insecticides against Bed Bugs: A Double-Blind Study

Goddard

2014

Insects

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Bed bugs are resurging throughout the world, and, thus, effective pest control strategies are constantly needed. A few studies have evaluated 25(b) and other natural, or so-called “green” products, as well as over-the-counter insecticides for bed bugs, but additional studies are needed to determine efficacy of bed bug control products. This double-blinded research project was initiated to examine long-term effectiveness of six commercially available natural or “green” insecticides against bed bugs and to compare them with three known traditional residual products. Water was used as a control. Products were evaluated against both susceptible and resistant strains of bed bugs (1200 bugs each), and two different substrates were used. Temprid® (Bayer Corporation, Monheim, Germany), Transport® (FMC Corp., Philadelphia, PA, USA), Invader® (FMC Corporation, Philadelphia, PA USA), Cimexa® (Rockwell Laboratories, Kansas City, MO, USA), and BBT-2000® (Swepe-Tite LLC, Tupelo, MS, USA) were the only products which showed any substantial (>40%) bed bug control upon exposure to treated substrates after the six-month waiting period, although results with the resistant bed bug strain were much reduced. Alpine dust® (BASF Corporation, Florham Park, NJ, USA) killed 27% of bed bugs or less, depending on strain and substrate. EcoRaider® (North Bergen, NJ, USA) and Mother Earth D® (Whitmire Microgen, Florham Park, NJ, USA) (diatomaceous earth) produced 11% control or less. Cimi-Shield Protect® (Pest Barrier, Carson, CA, USA) showed no activity against bed bugs in this study. Analysis using SAS software showed a three-way interaction between treatment, substrate, and bed bug strain (Numerator DF 9; Denominator DF 80; F = 4.90; p < 0.0001).

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“…BSA stored in dextranol-based matrix still resembled the frozen sample. Note that it is common to see higher molecular bands in SDS-PAGE of BSA due to irreversible multimer formation [53, 54].…”

Section: Resultsmentioning

confidence: 99%

Dextranol: An inert xeroprotectant

2019

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Dextranol, a reduced dextran, prevents damage to stored dry protein samples that unmodified dextran would otherwise cause. Desiccation protectants (xeroprotectants) like the polysaccharide dextran are critical for preserving dried protein samples by forming a rigid glass that protects entrapped protein molecules. Stably dried proteins are important for maintaining critical information in clinical samples like blood serum as well as maintaining activity of biologic drug compounds. However, we found that dextran reacts with both dried serum proteins and lyophilized purified proteins during storage, producing high-molecular weight Amadori-product conjugates. These conjugates appeared in a matter of days or weeks when stored at elevated temperatures (37° or 45°C), but also appeared on a timescale of months when stored at room temperature. We synthesized a less reactive dextranol by reducing dextran’s anomeric carbon from an aldehyde to an alcohol. Serum samples dried in a dextranol-based matrix protected the serum proteins from forming high-molecular weight conjugates. The levels of four cancer-related serum biomarkers (prostate specific antigen, neuropilin-1, osteopontin, and matrix-metalloproteinase 7) decreased, as measured by immunoassay, when serum samples were stored for one to two weeks in dextran-based matrix. Switching to a dextranol-based xeroprotection matrix slightly reduced the damage to osteopontin and completely stopped any detectable damage during storage in the other three biomarkers when stored for a period of two weeks at 45°C. We also found that switching from dextran to dextranol in a lyophilization formulation eliminates this unwanted reaction, even at elevated temperatures. Dextranol offers a small and easy modification to dextran that significantly improves the molecule’s function as a xeroprotectant by eliminating the potential for damaging protein-polysaccharide conjugation.

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A Ruthenium(II) Complex Supported by Trithiacyclononane and Aromatic Diimine Ligand as Luminescent Switch-On Probe for Biomolecule Detection and Protein Staining

Abstract: A new ruthenium(II) complex has been developed for detection of biomolecules. This complex is highly selective for histidine over other amino acids and has been applied to protein staining in an SDS-PAGE gel.

Cited by 26 publications

References 52 publications

Study of Reactive Melt Processing Behavior of Externally Plasticized Cellulose Acetate in Presence of Isocyanate

Study of Reactive Melt Processing Behavior of Externally Plasticized Cellulose Acetate in Presence of Isocyanate

Long-Term Efficacy of Various Natural or “Green” Insecticides against Bed Bugs: A Double-Blind Study

Dextranol: An inert xeroprotectant

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