A “sample-in-multiplex-digital-answer-out” chip for fast detection of pathogens

Yin, Juxin; Zou, Zheyu; Hu, Zhenming; Zhang, Shan; Zhang, Fengping; Wang, Ben; Lv, Shaowu; Mu, Ying

doi:10.1039/c9lc01143a

Cited by 105 publications

(66 citation statements)

References 38 publications

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“…To further increase the detection sensitivity, Yang et al (2018) built an integrated sample-in-digital-answer-out (SIDAO) disposable cartridge incorporating DNA extraction and digital recombinase polymerase amplification (dRPA) which can enable rapid and absolute quantitative nucleic acid analysis. Recently, Yin et al (2020) described an integrated multiplex dRPA microfluidic chip which can simultaneously detect three species of pathogenic bacteria within 45 min. Furthermore, the DNA amplification of RPA can be monitored in real-time using a low cost thin-film transistor (TFT) nanoribbon (NR) sensor ( Hu et al, 2017a , Hu et al, 2017b ).…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

350

309

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The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) worldwide pandemic. This unprecedented situation has garnered worldwide attention. An effective strategy for controlling the COVID-19 pandemic is to develop highly accurate methods for the rapid identification and isolation of SARS-CoV-2 infected patients. Many companies and institutes are therefore striving to develop effective methods for the rapid detection of SARS-CoV-2 ribonucleic acid (RNA), antibodies, antigens, and the virus. In this review, we summarize the structure of the SARS-CoV-2 virus, its genome and gene expression characteristics, and the current progression of SARS-CoV-2 RNA, antibodies, antigens, and virus detection. Further, we discuss the reasons for the observed false-negative and false-positive RNA and antibody detection results in practical clinical applications. Finally, we provide a review of the biosensors which hold promising potential for point-of-care detection of COVID-19 patients. This review thereby provides general guidelines for both scientists in the biosensing research community and for those in the biosensor industry to develop a highly sensitive and accurate point-of-care COVID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.

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Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

350

309

View full text Add to dashboard Cite

show abstract

“…One group recently integrated a digital amplification process into a biosensor based on a sample-response chip to quantify nucleic acids, further simplifying the entire nucleic acid analysis process [97] . After adding the sample, the cells are lysed and the nucleic acid is bound to the magnetic beads.…”

Section: Nanoscale Virus Biosensorsmentioning

confidence: 99%

Current progress on COVID-19 related to biosensing technologies: New opportunity for detection and monitoring of viruses

Laghrib

Saqrane

Bouabi

et al. 2021

Microchemical Journal

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Highlights COVID-19 infection poses a serious risk to human life by causing acute lung damage. Various techniques used to identify and quantify COVID-19 infection. Major challenges for containing the spread of COVID-19 is the ability to identify asymptomatic cases. Currently available diagnostic methods, biosensing technology developed during COVID-19 infection.

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“…In developing microfluidic devices for the analysis of food samples, one of the key requirements is that of developing miniaturized extraction techniques compatible with lab-on-a-chip and lab-on-paper platforms. The literature contains many proposals for the extraction of various substances in milk samples, including antibiotics [ 37 ] and foodborne pathogens [ 38 , 39 , 40 , 41 ]. For example, Park et al [ 39 ] presented an integrated rotary microfluidic system for performing the extraction and amplification of foodborne pathogens.…”

Section: Sample Pretreatment Of Microfluidic Devicesmentioning

confidence: 99%

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

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A “sample-in-multiplex-digital-answer-out” chip for fast detection of pathogens

Abstract: An integrated multiplex dRPA chip for rapid and accurate detection of foodborne bacteria.

Cited by 105 publications

References 38 publications

Detection of COVID-19: A review of the current literature and future perspectives

Detection of COVID-19: A review of the current literature and future perspectives

Current progress on COVID-19 related to biosensing technologies: New opportunity for detection and monitoring of viruses

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

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