A sandwich-type amperometric immunosensor fabricated by Au@Pd NDs/Fe2+-CS/PPy NTs and Au NPs/NH2-GS to detect CEA sensitively via two detection methods

Pei, Fubin; Wang, Ping; Ma, Enhui; Yu, Haoxuan; Gao, Chunxiao; Yin, Haohong; Li, Yueyun; Liu, Qing; Dong, Yunhui

doi:10.1016/j.bios.2018.09.065

Cited by 55 publications

(30 citation statements)

References 39 publications

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“…A variety of sensing strategies have been established to boost the reliability of analytical data. As shown in Figure 4, a facile sandwich-type immunosensor was developed on account of two electrochemical detection methods [47]. The synthesized amino-functionalized graphene sheet-supported Au nanoparticles (Au NPs/NH 2 -GS) with high electron transfer rate were cast on a GCE surface to constitute the sensing platform.…”

Section: Nanomaterialsmentioning

confidence: 99%

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

Jiang

Xia

Zang

et al. 2021

Sensors

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Recently, electrochemistry- and photoelectrochemistry-based biosensors have been regarded as powerful tools for trace monitoring of carcinoembryonic antigen (CEA) due to the fact of their intrinsic advantages (e.g., high sensitivity, excellent selectivity, small background, and low cost), which play an important role in early cancer screening and diagnosis and benefit people’s increasing demands for medical and health services. Thus, this mini-review will introduce the current trends in electrochemical and photoelectrochemical biosensors for CEA assay and classify them into two main categories according to the interactions between target and biorecognition elements: immunosensors and aptasensors. Some recent illustrative examples are summarized for interested readers, accompanied by simple descriptions of the related signaling strategies, advanced materials, and detection modes. Finally, the development prospects and challenges of future electrochemical and photoelectrochemical biosensors are considered.

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Section: Nanomaterialsmentioning

confidence: 99%

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

Jiang

Xia

Zang

et al. 2021

Sensors

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“…As example, gold@palladium nanoparticles loaded with molybdenum disulfide functionalized multiwalled carbon nanotubes (Au@Pd/MoS 2 @MWCNTs) have been proposed by Gao et al for the detection of hepatitis B e antigen (HBeAg) at levels of clinical interest (fg/mL) [110]. A particular case is the one reported by Pei et al in which the signal amplification label, gold@palladium nanodendrites loaded on ferrous-chitosan functionalized polypyrrole nanotubes (Au@Pd NDs/Fe 2+ -CS/PPy NTs) not only showed electrocatalytic activity towards H 2 O 2 reduction, but also was used as electroactive compound to directly amplify the current signal of Fe 2+ , without need of any additional substance [111]. Benefitting from both possibilities, amperometry and square wave voltammetry (SWV) were used, respectively, for the electrochemical detection of CEA at fg/mL levels (Figure 8C).…”

Section: Nanoparticles As Electrocatalytic Labelsmentioning

confidence: 99%

“…( C ) Scheme of the electrochemical immunosensor for CEA quantification based on the direct and catalytic detection of Au@Pd NDs/Fe 2+ -CS/PPy NTs. Adapted from [111] with permission.…”

Section: Figurementioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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This review shows recent trends in the use of nanoparticles as labels for electrochemical immunosensing applications. Some general considerations on the principles of both the direct detection based on redox properties and indirect detection through electrocatalytic properties, before focusing on the applications for mainly proteins detection, are given. Emerging use as blocking tags in nanochannels-based immunosensing systems is also covered in this review. Finally, aspects related to the analytical performance of the developed devices together with prospects for future improvements and applications are discussed.

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“…Carcinoembryonic antigen (CEA), a broadly studied tumor marker, is an attractive clinical cancer indicator in colon tumors, breast tumors, ovarian carcinomas, colorectal cancer and cystadenocarcinomas [ 3 ]. The sensitive and accurate detection of tumor markers in serum is vital for early diagnoses and clinical treatment of tumors [ 4 ]. To date, various sophisticated methods have been used to determine biomarkers, including immunohistochemistry (IHC) [ 5 ], real-time PCR [ 6 ], microarray chips [ 7 ], fluorescence in situ hybridization (FISH) [ 8 , 9 ], chromogenics [ 10 ], chemiluminescence immunoassay [ 11 , 12 ] and electrochemistry [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen

Wei

Wang

et al. 2021

Micromachines

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The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH2-GO) were the label of secondary antibodies (Ab2), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH2-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab2) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab2/PdAuCu NPs/NH2-GO catalyzed the reduction of H2O2 in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL−1 and a linear detection range from 0.1 pg mL−1 to 200 ng mL−1. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer.

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A sandwich-type amperometric immunosensor fabricated by Au@Pd NDs/Fe2+-CS/PPy NTs and Au NPs/NH2-GS to detect CEA sensitively via two detection methods

Cited by 55 publications

References 39 publications

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen

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