A Sandwich‐Type Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene Using Locked Nucleic Acids on Gold Electrode

Abstract

In this study, a sandwich-type electrochemical enzyme-based LNA-modified DNA biosensor was developed to detect relative gene in chronic Myelogenous Leukemia first. This biosensor is based on a sandwich detection strategy, which involves a pair of probes (a capture probe immobilized at the electrode surface and a reporter probe labeled biotin as an affinity tag for avidin-HRP) modified LNA. Since biotin can be connected with avidin-HRP, this biosensor offers an enzymatically amplified electrochemical current signal for the detection of target DNA. This new pattern exhibits high sensitivity and selectivity, and this biosensor has been used for an assay of PCR real sample with satisfactory result.

Cannabinoid receptor gene detection by electrochemical genosensor

“…The use of a signalling probe, in fact, offers several advantages: the elimination of possible secondary structures, provides sufficient rigidity to the target strand and allows an easy labelling with enzyme conjugates (i.e. streptavidin alkaline phosphatase) which come in close proximity to the electrode surface [21,[26][27][28]. This format have shown particularly suitable also for the discrimination of single base polymorphisms, once carefully selected position and length of the signalling probe sequence [26].…”

“…To our knowledge, only a few papers can be found in literature which describe the use of LNA probes for PCR amplicons detection [20,21] and they investigated lower ranges of concentrations. Our target was a region of the CB2 cannabinoid receptor gene (CNR2), which is relevant for the study of a mutation suspected to account for altered receptor activity.…”

“…New synthetic DNA analogues, such as peptide nucleic acid (PNA) and locked nucleic acid (LNA), have been recently designed to serve as new probes, able to hybridise with high stability and selectivity complementary DNA targets in the presence of mismatched sequences, thus allowing a rapid screening of clinically relevant single base polymorphisms (SNPs) [1,[19][20][21]. LNA is a nucleic acid analogue of RNA (Scheme 1), in which the sugar residue is chemically locked by the introduction of a methylene linkage between 2 0 -oxygen and 4 0 -carbon [22].…”

Disposable amperometric biosensors based on xanthine oxidase immobilized in the Prussian blue modified screen-printed three-electrode system

“…Although the XOD biosensors have been reported, most of them are based on conventional cylindrical electrodes which need high cost and complicated pretreatment, such as the glass carbon electrode [14][15][16], gold electrode [17,18], and carbon paste electrode [19,20]. This paper focuses on the application to introduce inexpensive screen-printed three-electrode system (SPTE), which can be self-fabricated in laboratory for mass production by printing carbonworking, carbon-counter and Ag/AgCl reference electrodes on polyethylene terephthalate substrate simultaneously.…”

A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method

“…[10][11][12][13] Already, many researchers have focused on improving the sensitivity and selectivity for the biosensor by designing the special probes or introducing advanced materials. [14][15][16][17] In our previous work, 3,18 we studied the electrochemical biosensor using a sandwich-type biosensor combined with enzyme-amplified technology, and found high sensitivity and selectivity. However, this one-step hybridization strategy was found to be disadvantageous for the special target sequences, which are easy to form secondary structure.…”