A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary

Chantawannakul, Panuwan; Ward, L. I.; Boonham, Neil; Brown, Mike

doi:10.1016/j.jip.2005.11.001

Cited by 133 publications

(97 citation statements)

References 13 publications

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“…Evidence from our and previous studies suggest that Varroa mites might not play a major role in transmitting this virus (Ball and Allen 1988;Carreck et al, 2002;Forgach et al, 2008;Locke et al, 2012;Ribière et al, 2008;Tentcheva et al, 2004a). However, BQCV was detected in Varroa mites from an apiary in Thailand (Chantawannakul et al, 2006) and it was found that Varroa mites can be vectors of BQCV (Ball, 1989;Johns, 2003). Consequently, more studies are required to clarify the relationship between V. destructor and BQCV (Chen and Siede, 2007).…”

Section: Discussionmentioning

confidence: 73%

Seasonal prevalence of pathogens and parasites in the savannah honeybee (Apis mellifera scutellata)

Strauss

Human

Gauthier

et al. 2013

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 73%

Seasonal prevalence of pathogens and parasites in the savannah honeybee (Apis mellifera scutellata)

Strauss

Human

Gauthier

et al. 2013

Journal of Invertebrate Pathology

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“…To detect viruses, qRT-PCR assays as described previously (Mumford et al, 2000;Chantawannakul et al, 2006) In all cases, negative and positive controls were included and analyzed in parallel to detect possible contamination and the good performance of the techniques.…”

Section: Analysis Of Main Honey Bee Pathogensmentioning

confidence: 99%

Risk factors associated with honey bee colony loss in apiaries in Galicia, NW Spain

Meana

Llorens-Picher

Euba

et al. 2017

Span. j. agric. res.

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A cross-sectional study was carried out in Galicia, NW Spain, in order to estimate the magnitude of honey bee colony losses and to identify potential risk factors involved. A total of 99 samples from 99 apiaries were collected in spring using simple random sampling. According to international guidelines, the apiaries were classified as affected by colony loss or asymptomatic. Each sample consisted of worker bees, brood and comb-stored pollen. All worker bees and brood samples were analysed individually in order to detect the main honey bee pathogens. Moreover, the presence of residues of the most prevalent agrotoxic insecticides and acaricides was assessed in comb-stored pollen. The general characteristics of the apiaries and sanitary information regarding previous years was evaluated through questionnaires, while the vegetation surrounding the apiaries sampled was assessed by palynological analysis of comb-stored pollen. The colony loss prevalence was 53.5% (CI 95% =43.2-63.9) and Nosema ceranae was found to be the only risk factor strongly associated with colony loss. The decision tree also pointed out the impact of the Varroa mite presence while variables such as apiary size, the incorrect application of Varroa mite treatments, and the presence of Acarapis woodi and Kashmir bee virus (KBV) were identified as possible co-factors.Additional key words: colony collapse; Apis mellifera; Nosema ceranae; Varroa destructor; imidacloprid; fipronil; comb-stored pollen.Correspondence should be addressed to Aránzazu Meana Mañes: ameana@ucm.es (Tables S1, S2 and S3) accompanies the paper on SJAR's website.

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“…DWV is a 30 nm icosahedral particle consisting of a single, positive strand RNA genome [8], typical for ifl aviruses, a genus of the family Ifl aviridae, within the order Picornavirales [9,10]. As widely and globally distributed, DWV is the most studied of all honeybee viruses [4,[11][12][13][14][15][16][17][18]. DWV has been detected in adult bees, their pupae and larvae [19], as well as in Varroa mites [12] and Tropilaelaps mercedesae [20].…”

Section: Introductionmentioning

confidence: 99%

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Simeunović¹,

Stevanović²,

Vidanović³

et al. 2014

Acta Veterinaria

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In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.

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A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary

Cited by 133 publications

References 13 publications

Seasonal prevalence of pathogens and parasites in the savannah honeybee (Apis mellifera scutellata)

Seasonal prevalence of pathogens and parasites in the savannah honeybee (Apis mellifera scutellata)

Risk factors associated with honey bee colony loss in apiaries in Galicia, NW Spain

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

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