A Search for CD36 Ligands from Flavor Volatiles in Foods with an Aldehyde Moiety: Identification of Saturated Aliphatic Aldehydes with 9–16 Carbon Atoms as Potential Ligands of the Receptor

Tsuzuki, Satoshi; Amitsuka, Takahiko; Okahashi, Tatsuya; Kimoto, Yusaku; Inoue, Kazuo

doi:10.1021/acs.jafc.7b01890

Cited by 8 publications

(19 citation statements)

References 45 publications

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“…It was also found that oxGPC SRB species, like 1-(palmitoyl)-2-(5-keto-6-octenedioyl)phosphatidylcholine (KOdiA-PC, one of the most potent lipid ligands of CD36), inhibited the binding of fl-oxLDL to the CD36 peptides (16,30,31), confirming that the peptides bind oxLDL particles by recognising their oxGPC SRB moiety. A novel finding of our studies using the CD36 peptides was that aliphatic aldehydes such as tetradecanal, a distinct class of volatile odorants, were capable of inhibiting fl-oxLDL binding to the mimics (i.e., were identified as potential CD36 ligands) (32,33,35). Furthermore, others and we have discovered that CD36 is expressed by a population of olfactory sensory neurons and is abundantly present in the olfactory ciliary layer in mice (17,22,38).…”

Section: Preparation Of Mimic Peptides For Class B Scavengermentioning

confidence: 76%

“…An oligopeptide containing amino acid residues 149-168 of human CD36 with biotin at the N-terminus was purchased from Life Technologies Japan (Tokyo), after synthesis (35). Peptides for SR-B1 [b5S-SRB1 187-206 , b5S-SRB1 187-206 (scramble) and b5S-SRB1 187-206 (3K/N)] were also purchased from the same vendor.…”

Section: Preparation Of Mimic Peptides For Class B Scavengermentioning

confidence: 99%

“…The procedure for labelling of human oxLDL with the fluorescent dye AlexFluor ® 488 was the same as described previously (32,33,35). The fl-oxLDL particles were stored in a light shielding vial at 4°C until use.…”

Section: Western Immunoblot Analysis Tissue Pieces Frommentioning

confidence: 99%

“…To this end, we devised an assay system to characterise SR-B1 ligands. We previously developed a cell-free in-vitro assay for the identification of CD36 ligands (29)(30)(31)(32)(33)35). In the assay, we used a solid support onto which certain synthetic peptides containing the oxLDL binding site of CD36 (Fig.…”

Section: Design and Development Of B5s-srb1 187-206 A Peptide Mimicmentioning

confidence: 99%

“…1B). This system allowed the estimation of CD36 ligand activity in test compounds by measuring their ability to inhibit fl-oxLDL binding to the support (30)(31)(32)(33)(34)(35). In this study, we devised a similar cell-free assay system for screening of SR-B1 ligands.…”

Section: Design and Development Of B5s-srb1 187-206 A Peptide Mimicmentioning

confidence: 99%

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A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity

et al. 2018

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Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. In this study, we performed a cell-free in-vitro assay utilising a peptide mimic of the receptor, and demonstrated that SR-B1 could recognise aliphatic aldehydes (e.g., tetradecanal), a distinct class of volatile odorants, as potential ligands. By reverse transcription-polymerase chain reaction and western immunoblot analyses, we detected the expression of SR-B1 mRNA and protein, respectively, in mouse olfactory tissue. Finally, we immunohistochemically mapped the distribution of SR-B1 in the surface layer of olfactory epithelium in vivo, which is the first line of odorant detection. These findings uncover a novel role for SR-B1 as a contributor to the capture of specific odorants in the nasal cavity of mammals.Class B scavenger receptors are cell-surface proteins, characterised by two predicted transmembrane spans, broad expression patterns and the ability to recognise diverse ligands (13). Of these, scavenger receptor B1 (SR-B1) was initially identified as comprising 509 amino-acid residues (5). An earlier study has showed that SR-B1 is expressed in several tissues including the liver and adipose tissues, and can serve as a receptor for low-density lipoprotein (LDL), acetylated LDL, oxidised LDL (oxLDL) and maleylated bovine serum albumin (BSA) (2). Later, SR-B1 was found to recognise several other substances such as high-density lipoprotein (HDL) (1) and anionic phospholipids (15). These findings suggested that SR-B1 primarily participates in lipid and lipoprotein metabolism in internal organs (36). As studied extensively, it functions as a physiologically relevant HDL receptor to mediate the selective delivery of HDL-cholesterol (i.e., HDL-cholesteryl ester) to the liver and steroidogenic tissues (1,13,20). Another member of class B scavenger receptors, cluster of differentiation 36 (CD36), which comprises 472 amino-acid residues, is also a multifaceted and multifunctional player in lipid and lipoprotein metabolism (13,27,28). CD36 is known to share with SR-B1 the ability to recognise distinct ligands,

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Section: Preparation Of Mimic Peptides For Class B Scavengermentioning

confidence: 76%

Section: Preparation Of Mimic Peptides For Class B Scavengermentioning

confidence: 99%

Section: Western Immunoblot Analysis Tissue Pieces Frommentioning

confidence: 99%

Section: Design and Development Of B5s-srb1 187-206 A Peptide Mimicmentioning

confidence: 99%

Section: Design and Development Of B5s-srb1 187-206 A Peptide Mimicmentioning

confidence: 99%

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A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity

et al. 2018

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CD36‐Binding Amphiphilic Nanoparticles for Attenuation of α‐Synuclein‐Induced Microglial Activation

Zhao

Francis

Song

et al. 2022

Advanced NanoBiomed Research

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Neuroinflammation is one of the hallmarks contributing to Parkinson's disease (PD) pathology, where microglial activation occurs as one of the earliest events, triggered by extracellular α‐synuclein (aSYN) binding to the cluster of differentation 36 (CD36) receptor. Herein, CD36‐binding nanoparticles (NPs) containing tartaric acid–based amphiphilic macromolecules (AMs) are rationally designed to inhibit this aSYN–CD36 binding. In silico docking reveals that four AMs with varying alkyl side chain lengths present differential levels of CD36 binding affinity and that an optimal alkyl chain length promotes the strongest inhibitory activity toward aSYN–CD36 interactions. In vitro competitive binding assays indicate that the inhibitory activity of AM‐based NPs plateaus at intermediate side chain lengths of 12 and 18 carbons, supporting the in silico docking predictions. These intermediate‐length AM NPs also has significantly stronger effects on reducing aSYN internalization and inhibiting proinflammatory molecules tumor necrosis factor α (TNF‐α) and nitric oxide from aSYN‐challenged microglia. All four NPs modulate the gene expression of aSYN‐challenged microglia, downregulating proinflammatory genes TNF, interleukin 6 (IL‐6), and IL‐1β, and upregulating anti‐inflammatory genes transforming growth factor β (TGF‐β) and Arg1 expression. Herein, overall, a novel polymeric nanotechnology platform is represented that can be used to modulate aSYN‐induced microglial activation.

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Higher Straight-Chain Aliphatic Aldehydes: Importance as Odor-Active Volatiles in Human Foods and Issues for Future Research

Tsuzuki

2019

J. Agric. Food Chem.

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Owing to their apparent lack of health significance, higher straight-chain aliphatic aldehydes, i.e., those having alkyl chains with more than six carbon atoms, have been largely neglected in food and nutraceutical research. However, they are an important class of odor-active volatiles in human foods. Indeed, certain aldehydes, such as hexanal, E-2-nonenal, and E,E-2,4decadienal, serve as key odorants in a range of our foods and drinks. This perspective describes the significance of higher straight-chain aliphatic aldehydes as food odorants, focusing on several representative ones, and raises the issues regarding these aldehydes to be addressed in the future.

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A Search for CD36 Ligands from Flavor Volatiles in Foods with an Aldehyde Moiety: Identification of Saturated Aliphatic Aldehydes with 9–16 Carbon Atoms as Potential Ligands of the Receptor

Cited by 8 publications

References 45 publications

<b>A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal </b><b>cavity </b>

<b>A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal </b><b>cavity </b>

CD36‐Binding Amphiphilic Nanoparticles for Attenuation of α‐Synuclein‐Induced Microglial Activation

Higher Straight-Chain Aliphatic Aldehydes: Importance as Odor-Active Volatiles in Human Foods and Issues for Future Research

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