A Selective and Reversible Fluorescent Probe for Zn²⁺ Detection in Living Cells

Abstract

A newly 8-aminoquioline based fluorescent probe (NQA) was designed and synthesized as a good turn-on fluorescence sensor to reversibly detect Zn 2 + . The probe can detect Zn 2 + in an aqueous solution with good selectivity without interference from other metal ions (Cd 2 + in particular). And the limit of detection was calculated to be 2.15 × 10 À 9 M. The probe can efficiently monitor the change of concentration of Zn 2 + in a broad pH range from 4.0 to 11.0. NQA can detection of Zn 2 + reversibly based on the coordination interaction. NQA is biocompatibility and can be used to monitor the concentration of Zn 2 + in living cells without the interference from other similar cations, especially Cd 2 + .[a] X. Figure 7. Confocal microscopy images of MCF-7 incubated with NQA (10.0 μM), further treated with Cd 2 + (1.0 equiv., 10.0 μM), then incubated with Zn 2 + (1.0 equiv., 10.0 μM). ChemistrySelectFull Papers

Facile synthesis of N,B-co-doped carbon dots with the gram-scale yield for detection of iron (III) and E. coli

“…After being shocked and incubated for 10 min, PL intensity at 412 nm was measured. Different pH values (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and incubation times (1-50 min) were used to optimize the experimental conditions. The sensitive and selective experiments were processed in triplicate to ensure reproducibility and accuracy of the method.…”

“…Carbon nanomaterials have been extensively used in various fields of basic science and technological application [1][2][3][4][5]. CDs, as new types of carbon-based nano-fluorophores, have attracted intensive research efforts because of their 5 Contributed equally to this article.…”

A Selective and Reversible Fluorescent Probe for Cu2+ and GSH Detection in Aqueous Environments

“…The plot of the fluorescence intensity versus Cu 2+ concentration exhibited a good linear relationship with R 2 =0.998. The limit of detection was calculated to be 39.4 nM from the equation LOD=3σ/k, [15,24–26] Which was much lower than the maximum allowable level of inorganic Cu 2+ in drinking water (1.3 ppm, ∼20 μM) permitted by the EPA (Environmental Protection Agency) [20]…”

Recent Developments in Fluorescent Materials for Heavy Metal Ions Analysis From the Perspective of Forensic Chemistry

“…Other fluorescent molecules have been also synthesized to achieve high sensitivity and improved water solubility for biosensing and bioimaging. An 8-aminoquioline-based fluorescent sensor was synthesized to detect Zn 2+ with a LOD of 2.15 nM, efficiently monitoring Zn 2+ changes in a broad pH range from 4.0 to 11.0 without interference from other metal ions and was biocompatible to monitor Zn 2+ in living cells (Chen et al, 2020b ). A highly sensitive fluorescent sensor based on tetraphenylethene (TPE)-functionalized quinolinium salts with aggregation-induced emission (AIE) characteristics was developed for the detection of I − and Hg 2+ , with a fluorescence “turn-on” signal for Hg 2+ (LOD of 71.8 nM) and “turn-off” for I − (LOD of 22.6 nM).…”