1998
DOI: 10.1006/bbrc.1998.8900
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A Selective Switch-on System for Self-Renewal of Embryonic Stem Cells Using Chimeric Cytokine Receptors

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Cited by 21 publications
(19 citation statements)
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“…The chemical-defined N2B27 medium was prepared as previously described [16] and the finalized 2i medium contained the addition of 1 µM PD0325901 (Stemgent, 04000610) and 3 µM CHIR99021 (Stemgent, 04000410) to N2B27 medium [9], [17]. The serum medium was prepared as previously described which contains 10 ng/ml leukemia inhibitory factor (LIF) (Millipore, LIF2010) and 15% fetal bovine serum (FBS) (Gibco, 10100147) [18].…”
Section: Methodsmentioning
confidence: 99%
“…The chemical-defined N2B27 medium was prepared as previously described [16] and the finalized 2i medium contained the addition of 1 µM PD0325901 (Stemgent, 04000610) and 3 µM CHIR99021 (Stemgent, 04000410) to N2B27 medium [9], [17]. The serum medium was prepared as previously described which contains 10 ng/ml leukemia inhibitory factor (LIF) (Millipore, LIF2010) and 15% fetal bovine serum (FBS) (Gibco, 10100147) [18].…”
Section: Methodsmentioning
confidence: 99%
“…The LIF receptor is a heterodimeric complex composed of a glycoprotein subunit gp130 and the receptor subunit LIFR (also called LIFRβ) (Ernst and Jenkins, 2004). Studies in mES have shown that the gp130 subunit is the essential component in transmitting self-renewal signals (Nakamura et al, 1998). Binding of LIF to the LIFR subunit induces dimerization with gp130, resulting in the formation of a high affinity receptor complex.…”
Section: Leukemia Inhibitory Factor (Lif)mentioning
confidence: 99%
“…1988). Binding of LIF and other members of the interleukin-6 family of cytokines to gp-130/LIFRβ heterodimer receptors on the surface of mouse ESCs (Stahl et Nakamura 1998) and subsequent activation of the Jak family kinase/Stat3 signaling pathway are required for the maintenance of pluripotency in an Oct4-independent manner (Niwa et Matsuda et al 1999). Independent of both these pathways, expression of Nanog, a divergent homeodomain gene is also required for the maintenance of pluripotency in ES and epiblast cells, while its role in PGCs has not yet been assessed Chambers et al 2003).…”
Section: Genes Involved In the Maintenance Of Pluripotency And Primormentioning
confidence: 99%