A Self-Priming Microfluidic Chip with Cushion Chambers for Easy Digital PCR

Xu, Gangwei; Si, Huaqing; Fu, Jing; Sun, Peng; Wu, Depei

doi:10.3390/bios11050158

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…In the future, combining complementary metal-oxide-semiconductor (CMOS) sensors used for fluorescence imaging would further enhance POCT capability [ 31 ]. In addition, our thermal cycler could be used for digital PCR assay by utilizing digitation methods, such as microwells [ 32 , 33 ], droplets [ 34 ], and microchannels [ 35 ]. Therefore, Ag/CF-film-based thermal cyclers can potentially be useful in the onsite detection of infectious diseases in resource-limited settings.…”

Section: Discussionmentioning

confidence: 99%

Conductive Silver/Carbon Fiber Films for Rapid Detection of Human Coronavirus

et al. 2022

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Polymerase chain reaction has gained attention since the outbreak of novel coronavirus in 2019. Due to its high specificity and capability for early detection, it is considered a standard method for the diagnosis of infectious diseases. However, the conventional thermocyclers used for nucleic acid amplification are not suitable for point-of-care testing applications, as they require expensive instruments, high-power consumption, and a long turnaround time. To suppress the widespread of the pandemic, there is an urgent need for the development of a rapid, inexpensive, and portable thermal cycler. Therefore, in this paper, we present a conductive silver/carbon fiber film-based thermal cycler with low power consumption (<5 W), efficient heating (~4.5 °C/s), low cost (<USD 200), and handheld size (11.5 × 7.1 × 7.5 mm). The conductive film, which was used as a heating source of the thermal cycler, was fabricated by the electrochemical deposition method. The successful coating of Ag was characterized by a scanning electron microscope and confirmed by energy-dispersive X-ray spectroscopy. The film showed excellent electrical/thermal conductivity and durability. Using our thermal cycler, 35 cycles of amplification were accomplished within 10 min. We also successfully demonstrated the multiplexed detection of various human coronaviruses (e.g., OC43, 229E, and NL63) using our thermal cycler.

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Section: Discussionmentioning

confidence: 99%

Conductive Silver/Carbon Fiber Films for Rapid Detection of Human Coronavirus

et al. 2022

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“…Like with ELISA, microwell arrays for nucleic acid detection can be loaded using a range of techniques. Flow cell loading [ 91 , 92 ], vacuum-assisted loading and oil-driven digitization [ 93 , 94 , 95 , 96 ], pressure loading [ 97 ], dispensing robots [ 98 , 99 ], and self-induced partitioning via SlipChip [ 100 , 101 , 102 ] have all been used for loading microwell arrays for nucleic acid applications.…”

Section: Digital Biosensing For Nucleic Acidsmentioning

confidence: 99%

Recent Advances in Digital Biosensing Technology

et al. 2022

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Digital biosensing assays demonstrate remarkable advantages over conventional biosensing systems because of their ability to achieve single-molecule detection and absolute quantification. Unlike traditional low-abundance biomarking screening, digital-based biosensing systems reduce sample volumes significantly to the fL-nL level, which vastly reduces overall reagent consumption, improves reaction time and throughput, and enables high sensitivity and single target detection. This review presents the current technology for compartmentalizing reactions and their applications in detecting proteins and nucleic acids. We also analyze existing challenges and future opportunities associated with digital biosensing and research opportunities for developing integrated digital biosensing systems.

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“…One of the most common materials for microfluidic channel fabrication for low scale manufacturing and prototyping is PDMS [ 103 ]. Often it is applied by casting onto the prefabricated master, and subsequently cured.…”

Section: Materials Considerationmentioning

confidence: 99%

Device Processing Challenges for Miniaturized Sensing Systems Targeting Biological Fluids

Stoukatch

Dupont

Redouté

2022

Biomedical Materials & Devices

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This article presents a review of device processing technologies used in the fabrication of biomedical systems, and highlights the requirements of advanced manufacturing technology. We focus on biomedical systems that perform diagnostics of fluidic specimens, with analytes that are in the liquid phase. In the introduction, we define biomedical systems as well as their versatile applications and the essential current trends. The paper gives an overview of the most important biomolecules that typically must be detected or analyzed in several applications. The paper is structured as follows. First, the conventional architecture and construction of a biosensing system is introduced. We provide an overview of the most common biosensing methods that are currently used for the detection of biomolecules and its analysis. We present an overview of reported biochips, and explain the technology of biofunctionalization and detection principles, including their corresponding advantages and disadvantages. Next, we introduce microfluidics as a method for delivery of the specimen to the biochip sensing area. A special focus lies on material requirements and on manufacturing technology for fabricating microfluidic systems, both for niche and mass-scale production segments. We formulate requirements and constraints for integrating the biochips and microfluidic systems. The possible impacts of the conventional microassembly techniques and processing methods on the entire biomedical system and its specific parts are also described. On that basis, we explain the need for alternative microassembly technologies to enable the integration of biochips and microfluidic systems into fully functional systems.

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A Self-Priming Microfluidic Chip with Cushion Chambers for Easy Digital PCR

Cited by 9 publications

References 28 publications

Conductive Silver/Carbon Fiber Films for Rapid Detection of Human Coronavirus

Conductive Silver/Carbon Fiber Films for Rapid Detection of Human Coronavirus

Recent Advances in Digital Biosensing Technology

Device Processing Challenges for Miniaturized Sensing Systems Targeting Biological Fluids

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