2000
DOI: 10.1128/aac.44.3.546-550.2000
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A Sensitive Amphotericin B Immunoassay for Pharmacokinetic and Distribution Studies

Abstract: Since currently used assays of amphotericin B (AMB) lack sensitivity or are not easily adaptable in all laboratories, we have developed an enzyme immunoassay for AMB in biological fluids and tissues. Antibodies to AMB were raised in rabbits after administration of an AMB-bovine serum albumin conjugate. The enzymatic tracer was obtained by coupling AMB via its amino group to acetylcholinesterase (EC 3.1.1.7). These reagents were used for the development of a competitive immunoassay performed on microtitration p… Show more

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Cited by 3 publications
(1 citation statement)
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“…Alternatively, absence of amphotericin B in the patient's brain tissue may reflect limitations of the bioassay analysis used (11). Recently, newer, more sensitive (> 100‐fold) immunoassay techniques (28) may help to improve these technical limitations.…”
Section: Discussionmentioning
confidence: 99%
“…Alternatively, absence of amphotericin B in the patient's brain tissue may reflect limitations of the bioassay analysis used (11). Recently, newer, more sensitive (> 100‐fold) immunoassay techniques (28) may help to improve these technical limitations.…”
Section: Discussionmentioning
confidence: 99%