A sensitive impedimetric DNA biosensor for the determination of the HIV gene based on electrochemically reduced graphene oxide

Gong, Qiaojuan; Yang, Haiying; Dong, Yanyun; Wen-chan, Zhang

doi:10.1039/c5ay00111k

Cited by 59 publications

(20 citation statements)

References 62 publications

(81 reference statements)

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“…Other electrochemical sensors utilizing covalently bound or tethered DNA probes have also been recently investigated for detecting HIV DNA, circulating tumor DNA, biotin-streptavidin interactions, and cardiac troponin I. [112][113][114][115] Biosensors using covalently linked strands are not limited to electrochemical methods. For fluorescent detection, one of the earliest reports came from our group in 2012.…”

Section: Covalently Linked and Tethered Probe Dnamentioning

confidence: 99%

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Covalent and Noncovalent Functionalization of Graphene Oxide with DNA for Smart Sensing

Lopez

Liu

2020

Advanced Intelligent Systems

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Interfacing nanomaterials with DNA has resulted in the development of numerous biosensors, optimized for different targets and applications. Of all nanomaterials, graphene oxide (GO) has emerged as a prime sensing platform due to its high specific surface area, good aqueous stability, varied functional groups and desirable surface, and electrical and optical properties. This review starts with an introduction of GO and describes its physical and chemical properties. Then, the general strategies of interfacing DNA and GO to develop sensors are discussed. The trends in GO/DNA biosensor development are organized into classes based on the mode of DNA interaction with GO (physisorbed vs chemisorbed). Due to the intermediate DNA adsorption strength on GO, most of the sensors developed utilize physisorption of DNA to GO. Even within the realm of physisorbed probes, there are multiple sensing methods: direct adsorption, inhibited adsorption, competitive adsorption with the use of blocking agents, and tethered adsorption containing a strongly adsorbing block of DNA. Covalently linked DNA probes are also used to increase the biosensor stability. Each of these sensors has its advantages and disadvantages and the designs are discussed with representative examples in detail.

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Section: Covalently Linked and Tethered Probe Dnamentioning

confidence: 99%

“…Other electrochemical sensors utilizing covalently bound or tethered DNA probes have also been recently investigated for detecting HIV DNA, circulating tumor DNA, biotin‐streptavidin interactions, and cardiac troponin I. [ 112–115 ]…”

Section: Covalently Linked and Tethered Probe Dnamentioning

confidence: 99%

Covalent and Noncovalent Functionalization of Graphene Oxide with DNA for Smart Sensing

Lopez

Liu

2020

Advanced Intelligent Systems

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“…Various electrochemical methods have been developed for the detection of the HIV-1 gene recently. Square wave voltammetry (Zhang et al 2010 ), differential pulse voltammetry (Li et al 2014 ), amperometry (Gao et al 2018 ), electrogenerated chemiluminescence (Poorghasem et al 2016 ) and electrochemical impedance spectroscopy (EIS) (Gong et al 2015 ) techniques were employed to design ultra-sensitive HIV-1 DNA biosensors. Most of them resulted in a limit of detection values of below pM concentrations for pM–nM detection ranges.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA

et al. 2020

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In this study, a highly sensitive, electrochemical, and label-free DNA impedimetric sensor was developed using carbonized glass fiber-coal tar pitch (GF-CTP) electrodes supported with gold nanoparticles (AuNPs) for the detection of HIV-1 gene. Thiol-modified GF-CTP electrodes were prepared using amine crosslinking chemistry and AuNPs were self-assembled obtaining highly conductive nanoelectrodes, GF-CTP-ATP-Au. All steps of electrode modifications were characterized using electrochemical, spectroscopic, and microscopic methods. GF-CTP-ATP-Au electrode was then modified with a capture DNA probe (C-ssDNA) and optimized with a target DNA probe in terms of hybridization time and temperature between 30 and 180 min and 20-50 °C, respectively. Finally, the analytic performance of the developed ssDNA biosensor was evaluated using electrochemical impedance spectroscopy. The calibration of the sensor was obtained between 0.1 pM and 10 nM analyte working range. The limit of detection was calculated using signal to noise ratio of 3 (S/N = 3) as 13 fM. Moreover, interference results for two noncomplementary DNA probes were also tested to demonstrate non-specific ssDNA interactions. An electrochemical label-free DNA impedimetric sensor was successfully developed using a novel GF-CTP-ATP-Au electrode. This study suggests that highly sensitive DNA-based biosensors can be developed using relatively lowcost carbonaceous materials.

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“…The standard diagnostic tests for HIV infection are blood tests such as enzyme immunoassay (EIA), enzyme‐linked immunosorbent assay (ELISA), and western blot test . These tests check for the presence of antibodies to HIV, but not for HIV itself.…”

Section: Introductionmentioning

confidence: 99%

A T7exonuclease‐assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA

et al. 2015

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We report a fluorescence polarization (FP) platform for human immunodeficiency virus (HIV) DNA detection based on T7exonuclease-assisted target recycling amplification with graphene oxide (GO) acting as a FP signal amplifier. In the sensing method, the presence of the target DNA leads to target recycling with the assistance of T7exonuclease, furthermore, the amplification products are absorbed onto the surface of GO, so the all FP values are enhanced by GO. More importantly, this FP sensor exhibits high detection sensitivity; under optimal conditions, the change in FP is linear with the concentration of the target DNA within a concentration range of 50-2000 pmol/L, and the detection limit of this method is as low as 38.6 pmol/L. This FP sensor also exhibits high selectivity, even single-base mismatched DNA can be effectively discriminated from complementary target DNA. Above all, the proposed FP sensor may serve as a general platform for the sensitive assay of disease-related genes.

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A sensitive impedimetric DNA biosensor for the determination of the HIV gene based on electrochemically reduced graphene oxide

Abstract: A sensitive impedimetric DNA biosensor for the determination of the HIV gene was developed by employing electrochemically reduced graphene oxide as a sensing platform.

Cited by 59 publications

References 62 publications

Covalent and Noncovalent Functionalization of Graphene Oxide with DNA for Smart Sensing

Covalent and Noncovalent Functionalization of Graphene Oxide with DNA for Smart Sensing

An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA

A T7exonuclease‐assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA

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