A sensitive ratiometric electrochemiluminescence biosensor for hypoxanthine detection by in situ generation and consumption of coreactants

Zuo, Fumei; Zhang, Han; Xie, Junping; Chen, Shihong; Yuan, Ruo

doi:10.1016/j.electacta.2018.03.132

Cited by 18 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a construction strategy overcomes the limitations of using exogenous co-reactants and the discommodity of oxidase-based determination in the traditional ECL ratiometric method. However, given that the luminous efficiency of this system suffered from the poor water solubility of PFO, the same group established another anodic luminophor luminol with favorable water solubility by using H2O2 as the co-reactant for the determination of the XOD substrate hypoxanthine (Hx) [76]. In the presence of Hx, XOD catalyzed oxidation, resulting in the in situ consumption and generation of dissolved O 2 and H 2 O 2 , which served as the co-reactants of rGO-CdTe QDs and luminol, respectively.…”

Section: Enzyme-based Ratiometric Strategymentioning

confidence: 99%

Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis

et al. 2022

View full text Add to dashboard Cite

The potential-resolved strategy has gradually demonstrated its distinct values in electrochemiluminescence (ECL) bio-sensing due to its superior characteristics, such as low instrument requirement, short assay time, and improved sample throughput, in conjunction with spatial-and spectrum-resolved techniques. It has recently been widely generalized into versatile multiple-signal ECL analytic platforms, especially in ratiometric and multiplex ECL sensors, in accordance with some specific principles. Furthermore, luminophore pairs with potential-and wavelength-resolved properties have been utilized to visualize biosensors that display multiple colors depending on analyte concentration. However, only a few comprehensive reports on the principles, construction, and application of various ECL sensors in potential-resolved schemes have been published. This review aims to recount the potential-resolved strategy applying to (a) ratiometric ECL sensors, (b) multiplex ECL sensors, and (c) multicolor ECL sensors and to discuss the distinctions and connections among the application principles of these strategies. Finally, the future prospects of ECL-based potential-resolved analysis are explored.

show abstract

Section: Enzyme-based Ratiometric Strategymentioning

confidence: 99%

Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Generally, the working principal of ratiometric ECL analysis involves two emitters at two independent potentials . The present report explores a new KAN detection approach by combining the resonance energy transform (RET) strategy with the dual-potential ratiometric method.…”

Section: Introductionmentioning

confidence: 99%

“…23 Generally, the working principal of ratiometric ECL analysis involves two emitters at two independent potentials. 24 The present report explores a new KAN detection approach by combining the resonance energy transform (RET) strategy with the dual-potential ratiometric method. One of the requirements of RET is overlap of the ECL emission spectrum of the donor and UV−vis absorption spectrum of the acceptor.…”

Section: ■ Introductionmentioning

confidence: 99%

Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection

Luo

Yang

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Based on luminol-capped Pt-tipped Au bimetallic nanorods (NRs) (L-Au-Pt NRs) as the anode emitter and SnS 2 quantum dots (QDs) hybrid Eu metal organic frameworks (MOFs) (SnS 2 QDs@Eu MOFs) as the cathode emitter, a dual-signal electrochemiluminescence (ECL) platform was designed for the ultrasensitive and highly selective detection of kanamycin (KAN). Using a dual-signal output mode, the ratiometric ECL aptasensor largely eliminates false-positives or false-negatives by self-calibration in the KAN assay process. To stimulate the resonance energy transform (RET) system, the KAN aptamer and complementary DNA are introduced for conjugation between the donor and acceptor. With the specific recognition of target KAN by its aptamer, L-Au-Pt NRs-apt partially peels off from the electrode surface. Eventually, the RET system is removed, leading to an increasing cathode signal and a decreasing anode signal. In view of this phenomenon, the ratiometric aptasensor can quantify KAN from 1 pM to 10 nM with a low detection limit of 0.32 pM. This dual-signal ECL aptasensor exhibits great practical potential in environmental monitoring and food safety.

show abstract

“…Previously reported methods of determination of hpx include high-performance liquid chromatography [ 25 , 26 , 27 , 28 ], capillary electrophoresis [ 29 , 30 ], spectrophotometry [ 31 , 32 ] and electrochemiluminescence [ 33 , 34 ], which are usually costly and laborious and require a long analysis time. On the other hand, electrochemical methods offer several advantages, such as relatively simple and cheap instrumentation, high selectivity and sensitivity, high stability and rapid response time [ 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Hypoxanthine from Inosine and Unusual Hydrolysis of Immunosuppressive Drug Azathioprine through the Formation of a Diruthenium(III) System

2021

View full text Add to dashboard Cite

Hypoxanthine (hpx) is an important molecule for both biochemistry research and biomedical applications. It is involved in several biological processes associated to energy and purine metabolism and has been proposed as a biomarker for a variety of disease states. Consequently, the discovery and development of systems suitable for the detection of hypoxanthine is pretty appealing in this research field. Thus, we have obtained a stable diruthenium (III) compound in its dehydrated and hydrated forms with formula [{Ru(µ-Cl)(µ-hpx)}2Cl4] (1a) and [{Ru(µ-Cl)(µ-hpx)}2Cl4]·2H2O (1b), respectively. This purine-based diruthenium(III) system was prepared from two very different starting materials, namely, inosine and azathioprine, the latter being an immunosuppressive drug. Remarkably, it was observed that an unusual azathioprine hydrolysis occurs in the presence of ruthenium, thus generating hypoxanthine instead of the expected 6-mercaptopurine antimetabolite, so that the hpx molecule is linked to two ruthenium(III) ions. 1a and 1b were characterized through IR, SEM, powder and single-crystal X-ray Diffraction and Cyclic Voltammetry (CV). The electrochemical studies allowed us to detect the hpx molecule when coordinated to ruthenium in the reported compound. The grade of sensitivity, repeatability and stability reached by this diruthenium system make it potentially useful and could provide a first step to develop new sensor devices suitable to detect hypoxanthine.

show abstract

A sensitive ratiometric electrochemiluminescence biosensor for hypoxanthine detection by in situ generation and consumption of coreactants

Cited by 18 publications

References 31 publications

Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis

Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis

Novel Dual-Signal Electrochemiluminescence Aptasensor Involving the Resonance Energy Transform System for Kanamycin Detection

Detection of Hypoxanthine from Inosine and Unusual Hydrolysis of Immunosuppressive Drug Azathioprine through the Formation of a Diruthenium(III) System

Contact Info

Product

Resources

About