A sensitive sandwich enzyme immunoassay for calcitonin gene-related peptide (CGRP): Characterization and application

Frobert, Yveline; Nevers, Marie‐Claire; Amadesi, Silvia; Volland, Hervé; Brune, Patrick; Geppetti, Pierangelo; Grassi, Jacques; Créminon, Christophe

doi:10.1016/s0196-9781(98)00172-7

Cited by 23 publications

(20 citation statements)

References 36 publications

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“…At the end of the experiment, tissues were blotted and weighed. Fractions were freeze-dried, reconstituted with assay buffer, and analyzed by enzyme immunoassays for CGRP-LI and SP-LI according to the methods reported previously (Frobert et al, 1999;Ricciardolo et al, 2000). The detection limits of the assays were 5 pg/ml CGRP and 2 pg/ml SP.…”

Section: Methodsmentioning

confidence: 99%

Ethanol Causes Inflammation in the Airways by a Neurogenic and TRPV1-Dependent Mechanism

Trevisani

Gazzieri²,

Benvenuti³

et al. 2004

The Journal of Pharmacology and Experimental Therapeutics

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Ethanol (EtOH) stimulates peptidergic primary sensory neurons via the activation of the transient receptor potential vanilloid-1 (TRPV1). EtOH is also known to trigger attacks of asthma in susceptible individuals. Our aim was to investigate whether EtOH produces airway inflammation via a TRPV1-dependent mechanism and to verify whether this effect is produced via a mechanism distinct from that of acetaldehyde (AcH). EtOH caused a Ca 2ϩ -dependent release of neuropeptides from guinea pigs airways, an effect that was inhibited by both capsaicin pretreatment and the TRPV1 antagonist capsazepine (CPZ). Furthermore, EtOH contracted isolated guinea pig bronchi, showing efficacy similar to that of carbachol: this effect of EtOH was sensitive to capsaicin pretreatment, tachykinin receptor blockade, and TRPV1 antagonism. The EtOH metabolite AcH also contracted isolated guinea pig bronchi, but this action was not affected by capsaicin pretreatment, tachykinin receptor, or TRPV1 antagonism. EtOH by intravenous or intragastric route of administration caused bronchoconstriction and increased plasma extravasation in the guinea pig airways, effects that were abolished selectively by CPZ. In conclusion, we have demonstrated that EtOH stimulates peptidergic primary sensory neurons in the guinea pig airways by TRPV1 activation. This excitatory effect of EtOH, distinct from that of AcH, results in neurogenic inflammatory responses that may contribute to the mechanism of EtOH-induced asthma.

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Section: Methodsmentioning

confidence: 99%

Ethanol Causes Inflammation in the Airways by a Neurogenic and TRPV1-Dependent Mechanism

Trevisani

Gazzieri²,

Benvenuti³

et al. 2004

The Journal of Pharmacology and Experimental Therapeutics

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“…Calcitonin gene-related peptide was assayed in duplicate by using a commercially available rat CGRP EIA kit (SPI-BIO, Massy Cedex, France) as described by Frobert et al (1999). The reason we used a rat assay was that CGRP is a highly conserved molecule and bovine CGRP has a high homology (>99%) with the rat CGRP (only one amino acid difference).…”

Section: Calcitonin Gene-related Peptidementioning

confidence: 99%

Strong relationships between mediators of the acute phase response and fatty liver in dairy cows

Ametaj¹,

Bradford²,

Bobe³

et al. 2005

Can. J. Anim. Sci.

156

126

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. 2005. Strong relationships between mediators of the acute phase response and fatty liver in dairy cows. Can. J. Anim. Sci. 85: [165][166][167][168][169][170][171][172][173][174][175]. The objective of this study was to investigate the relationship between activation of acute phase response and fatty liver in transition dairy cows. Fatty liver was induced in dairy cows by feeding 8 kg of cracked corn 1 mo before the expected day of parturition. Liver and blood samples were obtained at days -4, 3, 8, 12, 14, 22, 27, and 36 postpartum. Cows that developed fatty liver (n = 4) reached peak total lipids in the liver at day 12 postpartum with 11.4% (wet wt.) compared with 6.6% in control cows (n = 4). Cows with fatty liver had greater plasma tumor necrosis factor-alpha (TNF-α), nonesterified fatty acids (NEFA), and lower lactate concentrations than did control cows at day -4. During highest concentrations of total lipids in the liver, for at least one time-point, fatty-liver cows had greater concentrations of plasma serum amyloid A (SAA), haptoglobin, and NEFA and lower concentrations of calcitonin gene-related peptide (CGRP), prostaglandin E 2 (PGE 2 ), cortisol, and TNF-α than did control cows. Concentrations of total lipids in the liver at 12 d postpartum were correlated (a) positively with plasma TNF-α, SAA, and NEFA and negatively with plasma CGRP before calving, (b) positively with plasma SAA, haptoglobin, and NEFA and negatively with plasma PGE 2 , CGRP, total cholesterol, and glucose at days 3, 8, and 12 postpartum, and (c) negatively with concentrations of plasma glucose, lactate, and total bilirubin after day 12 postpartum. In conclusion, this study indicates that the acute phase response occurs in cows with fatty liver as well as strong relationships between mediators of immune response and fatty liver. Mots clés: Réponse phase aiguë, foie gras, vaches laitièresFatty liver is a common metabolic disorder that affects almost half of all dairy cows immediately after parturition.Despite much progress in understanding metabolic events during the development of fatty liver, the precise mechanisms of the pathology remain unclear. The conventional view is that fatty liver is characterized by excess liver triacylglycerols (TAG) arising from a negative energy balance after parturition (Bauchart et al. 1998). The negative energy balance evokes a major mobilization of non-esterified fatty acids (NEFA) from adipose tissue and increased uptake of

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“…Two isoforms of calcitonin gene-related peptide (CGRP), alpha-CGRP and beta-CGRP, are widely expressed in serum. 31,32 To study the selectivity of this assay, we tested the cross-reactivity of PCT to both isoforms of calcitonin; and our assay exhibited a little cross-reactivity, which was acceptable for the analysis.…”

Section: Discussionmentioning

confidence: 99%

Determining the Concentration of Procalcitonin Using a Magnetic Particles-based Chemiluminescence Assay for the Clinical Diagnosis of Sepsis

Rao

et al. 2013

ANAL. SCI.

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Our objective is to develop an assay based on magnetic particles (MPs) to determine the concentration of procalcitonin (PCT) using a chemiluminescence immunoassay (CLIA). Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) were used to label two different anti-procalcitonin (PCT) monoclonal antibodies. The labeled antibodies, the PCT antigen, and the anti-FITC antibody-coated MPs formed a double-sandwiched immunocomplex. The measured relative light units (RLUs) of ABEI in the substrate solution were directly proportional to the amount of PCT present in the samples. The proposed method was linear to 600 ng/mL with a detection limit of 0.03 ng/mL. The coefficient of variation (CV) was <5% and <6% for the intra-and inter-assay precision, respectively. The average recoveries were between 95 and 107%. The linearity-dilution effect gave a linear correlation coefficient of 0.9912. This proposed assay provided an alternative method to quantitatively measure PCT in serum for the diagnosis of sepsis.

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A sensitive sandwich enzyme immunoassay for calcitonin gene-related peptide (CGRP): Characterization and application

Cited by 23 publications

References 36 publications

Ethanol Causes Inflammation in the Airways by a Neurogenic and TRPV1-Dependent Mechanism

Ethanol Causes Inflammation in the Airways by a Neurogenic and TRPV1-Dependent Mechanism

Strong relationships between mediators of the acute phase response and fatty liver in dairy cows

Determining the Concentration of Procalcitonin Using a Magnetic Particles-based Chemiluminescence Assay for the Clinical Diagnosis of Sepsis

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