A sensitive three monoclonal antibodies based automatic latex particle-enhanced turbidimetric immunoassay for Golgi protein 73 detection

Xia, Yanyan; Shen, Han; Zhu, Yefei; Xu, Hao; Li, Zhiyang; Jin, Si

doi:10.1038/srep40090

Cited by 9 publications

(7 citation statements)

References 43 publications

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“…In addition, the GP73 content in serum from 80 cases of liver cancer and 80 healthy people was detected by latex‐enhanced immunoturbidimetric method based on polyclonal antibody, with 94.6% sensitivity and 72.4% specificity. The use of three monoclonal antibody‐based latex‐enhanced immunoturbidimetric assays has a 96.7% sensitivity and 93.3% specificity . These two methods can be more easily automated, and they are faster than ELISA therefore have very good clinical application prospects.…”

Section: Detection Methods For Gp73mentioning

confidence: 99%

“…It is also known as Golgi membrane protein I or Golgi phosphor protein 2. Because its relative molecular mass in polyacrylamide gel electrophoresis is 7.3 × 10 4 , it is also called GP73 . At present, many studies have found that the GP73 protein is associated with liver diseases, especially with liver cancer diseases; therefore, it is expected to become a new serological tumour marker for early diagnosis of liver cancer, which has attracted widespread attention from research scholars.…”

Section: Introductionmentioning

confidence: 99%

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Golgi protein 73 and its diagnostic value in liver diseases

et al. 2018

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Golgi protein 73 (GP73, also referred to as Golph 2) with 400 amino acids is a 73 kDa transmembrane glycoprotein typically found in the cis-Golg complex. It is primarily expressed in epithelial cells, which has been found upregulated in hepatocytes in patients suffering from both viral and non-viral liver diseases. GP73 has drawn increasing attention for its potential application in the diagnosis of liver diseases such as hepatitis, liver cirrhosis and liver cancer. Herein, we reviewed the discovery history of GP73 and summarized studies by many groups around the world, aiming at understanding its structure, expression, function, detection methods and the relationship between GP73 and liver diseases in various settings.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Detection Methods For Gp73mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Golgi protein 73 and its diagnostic value in liver diseases

et al. 2018

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“…PETIA is one of the homogeneous immunoassays well adopted in clinical research . The assay typically uses monodisperse latex beads of 100–200 nm in diameter to enhance light-scattering signals due to aggregation of the antibody-conjugated particles in the presence of target antigens in a sandwich configuration. − Sample concentrations of 0.5 mg/L or less have been measured with good precision and high reproducibility. Compared with other commonly used immunodiagnostic methods such as enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA), PETIA has several distinct advantages such as rapid (within 10 min), simple (single-step and washing-free), reproducible (small coefficient of variation), and automatable .…”

Section: Introductionmentioning

confidence: 99%

Antibody-Conjugated Nanodiamonds as Dual-Functional Immunosensors for In Vitro Diagnostics

Chen

Lam

et al. 2023

Anal. Chem.

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Nanodiamonds (NDs) are carbon nanoparticles with a large refractive index, a high density, and exceptional chemical stability. When excited by green light, they can emit bright red fluorescence from implanted nitrogen-vacancy (NV) centers. Taking advantage of these properties, we have developed antibody-conjugated NDs as in vitro diagnostic sensors for two complementary assays: particle-enhanced turbidimetric immunoassay (PETIA) and spin-enhanced lateral flow immunoassay (SELFIA). To achieve this goal, monocrystalline diamond powders (∼100 nm in diameter) with or without NV implantation were first treated in molten KNO3 to reduce their size and shape inhomogeneity, followed by surface carboxylation in strong oxidative acids and non-covalent conjugation with antibodies in water. PETIA and SELFIA were carried out separately with a microplate reader and a magnetically modulated fluorescence analyzer. Using C-reactive protein (CRP) as the target antigen, we found that anti-CRP-conjugated NDs exhibited high colloidal stability over 1 month at 4 °C in buffer solution. The limits of detection for 3 μL of CRP sample solution were 0.06 μg/mL and 1 ng/mL with variation coefficients of less than 10 and 15% for PETIA and SELFIA, respectively. These two methods together provide a detection range of 1 ng/mL–10 μg/mL, potentially useful for clinical applications. This work represents the first practical use of rounded monocrystalline NDs as in vitro diagnostic reagents.

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“…In short, the detection is expected to proceed in the antibody excess zone, where the antibody–antigen precipitation has positive correlation with the antigen. At the present time, the conjugations of the reagent with nanomaterials such as latex particles [ 25 , 26 ] and nanogold [ 27 , 28 ] have been made, to enhance immunoturbidimetry sensitivity, but the biochemical conjugation process is relatively complicated. The convenient, simple and easy-to-operate NanoPITA method can be more suitable for ultrasensitive, rapid, and accurate analysis of protein markers, showing great potential in early clinical diagnosis of cardiovascular disease.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Detection of C-Reactive Protein by a Novel Nanoplasmonic Immunoturbidimetry Assay

Dang

Zhao

et al. 2022

Biosensors

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Nanotechnology has attracted much attention, and may become the key to a whole new world in the fields of food, agriculture, building materials, machinery, medicine, and electrical engineering, because of its unique physical and chemical properties, including high surface area and outstanding electrical and optical properties. The bottom-up approach in nanofabrication involves the growth of particles, and we were inspired to propose a novel nanoplasmonic method to detect the formation of nanoparticles in real time. This innovative idea may contribute to the promotion of nanotechnology development. An increase in nanometer particle size leads to optical extinction or density (OD)-value changes in our nanosensor chip at a specific wavelength measured in a generic microplate reader. Moreover, in applying this method, an ultrasensitive nanoplasmonic immunoturbidimetry assay (NanoPITA) was carried out for the high-throughput quantification of hypersensitive C-reactive protein (CRP), a well-known biomarker of cardiovascular, inflammatory, and tumor diseases. The one-step detection of the CRP concentration was completed in 10 min with high fidelity, using the endpoint analysis method. The new NanoPITA method not only produced a linear range from 1 ng/mL to 500 ng/mL CRP with the detection limit reduced to 0.54 ng/mL, which was an improvement of over 1000 times, with respect to regular immunoturbidity measurement, but was also effective in blood detection. This attractive method, combined with surface plasmon resonance and immunoturbidimetry, may become a new technology platform in the application of biological detection.

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A sensitive three monoclonal antibodies based automatic latex particle-enhanced turbidimetric immunoassay for Golgi protein 73 detection

Cited by 9 publications

References 43 publications

Golgi protein 73 and its diagnostic value in liver diseases

Golgi protein 73 and its diagnostic value in liver diseases

Antibody-Conjugated Nanodiamonds as Dual-Functional Immunosensors for In Vitro Diagnostics

Ultrasensitive Detection of C-Reactive Protein by a Novel Nanoplasmonic Immunoturbidimetry Assay

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