“…Allele sharing among contributors to a mixture and masking of alleles due to STR stutter artifacts can lead to inaccurate NoC estimates based on simply counting the number of alleles at a locus. Different approaches and software programs have been used for NoC estimation [ [269] , [270] , [271] , [272] , [273] , [274] , [275] ]. Total allele count (TAC) distribution via TAC curves showed an improvement in manually estimating the number of contributors with complex mixtures [ 276 ].…”