A serologic weakly reactive RhD is caused by a novel RHD (c.722C>A, p.Thr241Asn) allele

Abstract

Weak D phenotypes occur predominantly due to amino acid changes in the transmembrane or intracellular regions of the RhD protein. 1 Here we have identified a novel variant RHD allele in a 26-year-old G2P1 pregnant Chinese woman with a weak D phenotype.

A novel RHD allele caused by c.687_689delAAG variant

“…After informed consent, genomic DNA was isolated from peripheral blood using the TIANamp blood DNA kit (Tiangen Biotech Co., Ltd, China). RHD zygosity was assessed by sequence‐specific primer polymerase chain reaction (SSP‐PCR), as previously reported 3 . DNA sequencing of RHD exons 1 to 10 and flanking intron regions was performed by the Sanger dideoxy method using a cycle‐sequencing kit (Tianjin Super Biotechnology Development Co., Ltd, China).…”