A Serum Factor in Lupus Erythematosus with Affinity for Tissue Nuclei

Holborow, E. J.; Weir, D. M.; Johnson, G. D.

doi:10.1136/bmj.2.5047.732

Cited by 257 publications

(82 citation statements)

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“…Antinuclear antibodies were measured by using the method of Holborrow et a1 (16) with mouse liver as substrate. Antibodies to single-stranded DNA (ssDNA) and to double-stranded DNA (dsDNA) were determined by using 'zsI-ss-calf thymus DNA and 'H-T7 bacteriophage.…”

Section: Methodsmentioning

confidence: 99%

Early complement component depletion and mixed cryoglobulinemia in a “healthy” family

Waterman

Winkelstein

Berzofsky

et al. 1979

Arthritis & Rheumatism

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A family containing two apparently healthy brothers with profound early complement component depletion (Cl, C4, C2) and mixed IgG-IgM polyclonal cryoglobulIns was studied. The ctyoglobulins possessed rheumatoid factor activity and depleted early complement components in normal human serum. plement system. These deficiency states, both hereditary and acquired, are often associated with autoimmune diseases, most notably systemic lupus erythematosus (SLE) or a lupus-like syndrome (1). Cold precipitable proteins or cryoglobulins, presumably reflecting immune complex phenomena, have been found in many patients with hypocomplementemia (2). Cryoglobulins have also been associated with a variety of infectious, lymphoproliferative, and autoimmune diseases (3). An "essential" form of cryoglobulinemia, first described by Meltzer and Franklin (4,5), has also been described where the cryoglobulins are typically mixed IgG-IgM complexes which demonstrate rheumatoid factor activity and deplete early complement components (6). Clinical symptoms usually occur in this setting and include arthralgias, purpura, weakness and, frequently, glomerulonephritis (4,5).The present report describes the first cases of two apparently healthy members of the same family with profound early complement component deficiency and mixed cryoglobulinemia. MATERIALS AND METHODSIndex case report. The proband, a 36-year-old white man, was referred for evaluation of possible rheumatoid arthritis. Twelve years earlier he had abrupt onset of polyarthritis affecting small joints of the hands, wrists, elbows, shoulders, hips, knees, and ankles. During that evaluation a rheumatoid factor test was positive, and the diagnosis of rheumatoid arthritis was made. However, his articular syndrome resolved after 3 4 weeks, and he remained well for the next 12 years, except for occasional hrthralgias and the development of a mechanical low back syddrome. It was because of this axial skeletal complaint coupled with the presence of a persistently positive rheumatoid factor test and hypocomplementemia that he was referred. He denied any constitutional

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Section: Methodsmentioning

confidence: 99%

Early complement component depletion and mixed cryoglobulinemia in a “healthy” family

Waterman

Winkelstein

Berzofsky

et al. 1979

Arthritis & Rheumatism

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“…DNA) were first described in 1957 (1,2 ). Detection of anti-ds-DNA antibody (ds-DNA-Ab) is an integral part of the definition of systemic lupus erythematosus (SLE) as proposed by the American College of Rheumatology (ACR) criteria (3 ).…”

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confidence: 99%

ROC Analysis Comparison of Three Assays for the Detection of Antibodies against Double-Stranded DNA in Serum for the Diagnosis of Systemic Lupus Erythematosus

et al. 2004

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“…A pesquisa destes auto-anticorpos não está mais restrita à IFI, pois essa técnica pode não detectar alguns anticorpos, levando a resultado falso negativo (2,23) . A IFI é um importante teste de triagem, e teve sua sensibilidade aumentada quando se passou a utilizar como substrato as células de carcinoma de laringe humana (HEp-2) em substituição aos imprints e às secções de congelação de fígado e rim de roedores (10,24) . Este aumento da sensibilidade deve-se ao fato de as células HEp-2, por estarem em constante divisão, expressarem antígenos inexistentes nas células hepáticas e renais dos roedores.…”

Section: Discussionunclassified

A importância da contra-imunoeletroforese na detecção de antígenos nucleares extraíveis para o diagnóstico de doenças reumáticas sistêmicas

Bruder

Prudente²,

Silva³

et al. 2004

J. Bras. Patol. Med. Lab.

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y unitermos key words resumoOs antígenos nucleares extraíveis (ENAs) são encontrados no soro da maioria dos pacientes com doença reumática sistêmica. Os principais ENAs estudados são SS-A/Ro, SS-B/La, RNP, Sm, Scl-70 e Jo-1. Objetivou-se neste trabalho: a) padronizar a técnica de contra-imunoeletroforese (CIE) para a detecção de ENAs; b) padronizar o substrato antigênico (ENAs) para a CIE a partir de baço de cão; c) comparar os resultados da CIE com as técnicas de imunofluorescência indireta (IFI) e Elisa para esses antígenos. Para tal foram estudados 40 soros de pacientes com doença reumática sistêmica confirmada por exames clínico e laboratorial (sorológico e biópsia). Como controle negativo foram utilizados dez soros de doadores de sangue normais, e, como controles positivos, seis soros-padrão anti-Ro, anti-La, anti-RNP, anti-Sm, anti-Scl-70, anti-Jo-1, para caracterizar os antígenos presentes no extrato de baço. Neste último foram detectados vários ENAs, exceto RNP e Scl-70. A técnica de CIE apresentou boas sensibilidade (70%) e especificidade (100%) em relação às outras técnicas (IFI e Elisa). A titulação dos soros pela CIE revelou positividade até diluições de 1:16 em 32,5% dos casos. Concluímos que a CIE e os antígenos extraídos de baço de cão podem ser utilizados na rotina laboratorial para triagem destes ENAs, com a vantagem, em relação à IFI, de poderem ser titulados. Auto-anticorpos Testes sorológicos Antígenos nucleares extraíveis Contra-imunoeletroforese abstract Extractable nuclear antigens (ENAs) are detected in the sera of the majority of patients with rheumatic systemic disease. The main ENAs studied are SS-A/Ro, SS-B/La, RNP, Sm, Scl-70 and Jo-1. The aim of this work was: a) to standardize the counterimmunoelectrophoresis technique (CIE) to detect ENAs; b) to standardize the extration of ENAs from dog spleen as a substract for CIE test; c) to compare the results obtained by CIE with those of indirect immunofluorescence (IFI) and Elisa for these antigens. Forty sera from individuals with rheumatic systemic disease confirmed by clinical and serological tests and by skin and/or kidney biopsies were tested. Ten normal sera from blood donors were used as negative control, and as positive control to check for ENAs in the spleen extract, six positive sera for anti

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A Serum Factor in Lupus Erythematosus with Affinity for Tissue Nuclei

Abstract: and Schedel, H. E. (1838). Abrigd pratique des maladies de la peau, 3rd ed. Paris.

Cited by 257 publications

References 4 publications

Early complement component depletion and mixed cryoglobulinemia in a “healthy” family

Early complement component depletion and mixed cryoglobulinemia in a “healthy” family

ROC Analysis Comparison of Three Assays for the Detection of Antibodies against Double-Stranded DNA in Serum for the Diagnosis of Systemic Lupus Erythematosus

A importância da contra-imunoeletroforese na detecção de antígenos nucleares extraíveis para o diagnóstico de doenças reumáticas sistêmicas

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