A sex determination protocol for the Iberian desman (Galemys pyrenaicus) based on a three primer amplification of DBX and DBY fragments with non-invasive samples

Vidal, Oriol; Pérez‐Serra, Alexandra; Plá, Carles

doi:10.1007/s10592-009-9913-1

Cited by 3 publications

References 11 publications

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Determination of Sex Origin of Meat and Meat Products on the DNA Basis: A Review

Gokulakrishnan

Kumar

Sharma

et al. 2013

Critical Reviews in Food Science and Nutrition

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Sex determination of domestic animal's meat is of potential value in meat authentication and quality control studies. Methods aiming at determining the sex origin of meat may be based either on the analysis of hormone or on the analysis of nucleic acids. At the present time, sex determination of meat and meat products based on hormone analysis employ gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS), and enzyme-linked immunosorbent assay (ELISA). Most of the hormone-based methods proved to be highly specific and sensitive but were not performed on a regular basis for meat sexing due to the technical limitations or the expensive equipments required. On the other hand, the most common methodology to determine the sex of meat is unquestionably traditional polymerase chain reaction (PCR) that involves gel electrophoresis of DNA amplicons. This review is intended to provide an overview of the DNA-based methods for sex determination of meat and meat products.

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Determination of Sex Origin of Meat and Meat Products on the DNA Basis: A Review

Gokulakrishnan

Kumar

Sharma

et al. 2013

Critical Reviews in Food Science and Nutrition

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Molecular Method Based on Hydrolysis Probe Assays to Identify the Sex Chromosomes of Iberian Desman (Galemys pyrenaicus) Using Non‐Invasive Sampling

Ripa,

Palacios‐Gonzalez,

Díaz‐Caballero

et al. 2024

Integr. Zool.

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Desmans belong to the subfamily Desmaninae, which are members of the family Talpidae. Desmans and moles show limited sexual dimorphism, making unclear sex discrimination by phenotypic assessment. The Iberian desman (Galemys pyrenaicus) is an endangered species with a severe population decline. Knowledge of sex and sex ratio is essential for conservation and management. Based on these arguments and although previous conventional PCR studies amplifying DBX/DBY genes were relatively successful in sexing the desman, high‐resolution sex‐specific PCR has been requested. All these reasons encouraged us to develop new species‐specific RT‐qPCR assays by TaqMan probes to determine the sex in desman, especially with genetic material from non‐invasive samples. Accordingly, efficiency, limit of detection (LOD), specificity, and DNA analysis from faeces were verified. The target genes DBX and DBY were amplified with gDNA from both sexes, with Y‐chromosome consistently absent in the female. Despite the modest efficiency, regression analysis (R2 > 0.999) indicated a linear range of the DBX and DBY assays extending from 20 to 0.2 ng/µL DNA. LOD analyses estimated that twice as much gDNA was needed in males as in females for DBX detection. Paradoxically, the Y‐chromosome required three times as much gDNA as the X‐chromosome using a male sample. Therefore, an unexpected dosage imbalance in the genome in favour of the X chromosome was discussed in light of an apparent multicopy nature of the DBX gene and with a sexing success rate of 49.9% of the non‐invasive samples, supporting Fisher's principle for the mammalian XX/XY sex system, as expected.

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Desmán ibérico – Galemys pyrenaicus (E. Geoffory, 1811)

Nores¹

2017

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A sex determination protocol for the Iberian desman (Galemys pyrenaicus) based on a three primer amplification of DBX and DBY fragments with non-invasive samples

Cited by 3 publications

References 11 publications

Determination of Sex Origin of Meat and Meat Products on the DNA Basis: A Review

Determination of Sex Origin of Meat and Meat Products on the DNA Basis: A Review

Molecular Method Based on Hydrolysis Probe Assays to Identify the Sex Chromosomes of Iberian Desman (Galemys pyrenaicus) Using Non‐Invasive Sampling

Desmán ibérico – Galemys pyrenaicus (E. Geoffory, 1811)

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