Class room anxiety, stemming from academic pressure and fear of evaluation, impacts students' well-being and academic performance, underscoring the necessity for supportive learning environments. Objective: To assess the Class room anxiety level among nursing students. Methods: This cross-sectional study was undertaken at a private nursing institute in Karachi, Pakistan, from November 2023 to January 2024. Utilizing a convenient sampling technique, the study comprised one hundred twenty student participants. Results: The study's demographics reveal that 91.7% (n=110) of participants are aged 18-24, with a male majority of 83.3% (n=100). In terms of education year, 26.7% (n=32) are in the first year, 37.5% (n=45) in the third year, and 30.8% (n=37) in the fourth year. Regarding Class room anxiety, 7.5% (n=8) exhibited normal levels, 26.7% (n=32) showed mild to moderate anxiety, 55.0% (n=66) experienced severe anxiety, and 10.8% (n=13) reported extreme anxiety. Conclusions: This underscores the urgent need for targeted interventions in educational settings and emphasizes the ongoing importance of researching contributing factors for effective strategies to promote a healthier learning experience.