A short term analysis of the behaviour of conditionally immortalized neuronal progenitors and primary neuroepithelial cells implanted into the fetal rat brain

Cattaneo, Elena; Magrassi, Lorenzo; Butti, G.; Santi, Laura De; Giavazzi, Alessio; Pezzotta, S.

doi:10.1016/0165-3806(94)00137-5

Cited by 71 publications

(35 citation statements)

References 50 publications

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“…Shifting to the nonpermissive temperature of 39°C leads to degradation of the Large-T Antigen, with consequent block of cell proliferation and reversal of the phenotype back to that of a differentiating neuroblast. This was better demonstrated in in vivo transplantation studies (Cattaneo et al, 1994;Lundberg et al, 1997;Benedetti et al, 2000). When ST14A cells were exposed in vitro to 39°C in serum-deprived medium (SDM), cells changed morphology but also their viability decreased with time in a highly reproducible manner (Fig.…”

Section: Wt and Muhtt Differentially Affect Cell Viabilitymentioning

confidence: 89%

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Wild-Type Huntingtin Protects from Apoptosis Upstream of Caspase-3

et al. 2000

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Expansion of a polyglutamine sequence in the N terminus of huntingtin is the gain-of-function event that causes Huntington's disease. This mutation affects primarily the medium-size spiny neurons of the striatum. Huntingtin is expressed in many neuronal and non-neuronal cell types, implying a more general function for the wild-type protein. Here we report that wild-type huntingtin acts by protecting CNS cells from a variety of apoptotic stimuli, including serum withdrawal, death receptors, and pro-apoptotic Bcl-2 homologs. This protection may take place at the level of caspase-9 activation. The full-length protein also modulates the toxicity of the poly-Q expansion. Cells expressing full-length mutant protein are susceptible to fewer death stimuli than cells expressing truncated mutant huntingtin.

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Section: Wt and Muhtt Differentially Affect Cell Viabilitymentioning

confidence: 89%

“…ST14A cells were derived from the embryonic striatum via retroviral transduction of the temperature-sensitive version of the Large-T Antigen (Cattaneo et al, 1994;Cattaneo and Conti, 1998). ST14A cells grow in 10% serum at the permissive temperature of 33°C (Fig.…”

Section: Wt and Muhtt Differentially Affect Cell Viabilitymentioning

confidence: 99%

Wild-Type Huntingtin Protects from Apoptosis Upstream of Caspase-3

et al. 2000

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“…The surgical manipulation of rat embryos in utero was performed according to a previously described approach (Cattaneo et al, 1994;Carletti et al, 2002). Briefly, timed-pregnant E15 rats were deeply anesthetized, and the uterine horns were exposed.…”

Section: Methodsmentioning

confidence: 99%

Different Types of Cerebellar GABAergic Interneurons Originate from a Common Pool of Multipotent Progenitor Cells

et al. 2006

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Different cerebellar phenotypes are generated according to a precise spatiotemporal schedule, in which projection neurons precede local interneurons. Glutamatergic neurons develop from the rhombic lip, whereas GABAergic neurons originate from the ventricular neuroepithelium. Progenitors in these germinal layers are committed toward specific phenotypes already at early ontogenetic stages. GABAergic interneurons are thought to derive from a subset of ventricular zone cells, which migrate in the white matter and proliferate up to postnatal life. During this period, different interneuron categories are produced according to an inside-out sequence, from the deep nuclei to the molecular layer (we show here that nuclear interneurons are also born during late embryonic and early postnatal days, after glutamatergic and GABAergic projection neurons). To ask whether distinct interneuron phenotypes share common precursors or derive from multiple fate-restricted progenitors, we examined the behavior of embryonic and postnatal rat cerebellar cells heterotopically/ heterochronically transplanted to syngenic hosts. In all conditions, donor cells achieved a high degree of integration in the cerebellar cortex and deep nuclei and acquired GABAergic interneuron phenotypes appropriate for the host age and engraftment site. Therefore, contrary to other cerebellar types, which derive from dedicated precursors, GABAergic interneurons are produced by a common pool of progenitors, which maintain their full developmental potentialities up to late ontogenetic stages and adopt mature identities in response to local instructive cues. In this way, the numbers and types of inhibitory interneurons can be set by spatiotemporally patterned signals to match the functional requirements of developing cerebellar circuits.

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“…Although this possibility cannot be completely ruled out, other authors have shown that immortalized cell lines generated following the methodology we used to obtain the ST14A cells are capable of either proliferation in serum or differentiation upon stimulation of transfected neurotrophin receptors (31). Data on the capability of ST14A cells to differentiate are provided by intracerebral transplantation experiments in which ST14A cells were shown to become growth restricted (27) and to differentiate morphologically and biochemically into neurons and glial cells (11). 3 Furthermore, the IL3R-expressing clone (␣␤14 cells) as well as parental ST14A cells have been found to differentiate and to express neuronal antigens when exposed in vitro to SFM conditions (20).…”

Section: Discussionmentioning

confidence: 99%

“…For Western blot analyses, the striatum primordia were dissected from E14 rat embryos as described previously (27). Membranes of total cell lysates from ST14A and ␣␤14 cells, as well as those from the embryonic striatum, were reacted with anti-Jak1 (1:1000; Transduction Lab.…”

Section: Methodsmentioning

confidence: 99%

Activation of the JAK/STAT Pathway Leads to Proliferation of ST14A Central Nervous System Progenitor Cells

Cattaneo

Fraja

Conti

et al. 1996

Journal of Biological Chemistry

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We were interested in whether central nervous system progenitor cells possess the signal transduction machinery necessary to mediate cytokine functions and whether this machinery can become activated upon stable expression of a particular cytokine receptor. For this purpose we utilized a previously obtained conditionally immortalized striatum-derived nestin-positive cell line (ST14A). We found that ST14A cells express Jak2, but not Jak1 or Tyk2. An identical pattern of expression was found in embryonic striatal tissue. To evaluate the susceptibility of these cytokine specific cytoplasmic transducers to activation, ST14A cells were stably transfected with the ␣ and ␤ (AIC2A) chains of the murine interleukin-3 receptor. Four independent lines expressing both the ␣ and ␤ receptor subunits were obtained. We found that cells from each of these lines were induced to proliferate upon exposure to interleukin-3. Dose response curve, antibody blocking experiments and binding studies revealed that the response was mediated by the reconstituted high affinity interleukin-3 receptor. Immunoprecipitation studies on these cells showed that Jak2 and Stat5 were being phosphorylated after stimulation of the reconstituted receptor. These results indicate that members of the JAK/STAT family of proteins are expressed in central nervous system progenitor cells and are susceptible to activation through stimulation of an exogenously expressed cytokine receptor, ultimately leading to cell proliferation.

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A short term analysis of the behaviour of conditionally immortalized neuronal progenitors and primary neuroepithelial cells implanted into the fetal rat brain

Cited by 71 publications

References 50 publications

Wild-Type Huntingtin Protects from Apoptosis Upstream of Caspase-3

Wild-Type Huntingtin Protects from Apoptosis Upstream of Caspase-3

Different Types of Cerebellar GABAergic Interneurons Originate from a Common Pool of Multipotent Progenitor Cells

Activation of the JAK/STAT Pathway Leads to Proliferation of ST14A Central Nervous System Progenitor Cells

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