A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products.

Heyderman, Eadie; Neville, A. Munro

doi:10.1136/jcp.30.2.138

Cited by 165 publications

(50 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Triton does cause some damage to liver parenchymal cells (Cotmore & Carter, 1973), the effect on the galactosereceptor function was minimal and asialo-glycoprotein uptake was normal, though it is possible that subsequent inhibition of lysosomal digestion of the glycoproteins may have occurred. It can be seen from the above data that removal of sialic acid from carcinoembryonic antigen in itself does not affect Kupffer-cell uptake, but when terminal galactose residues are exposed the uptake by hepatocytes becomes important and in general the majority of the circulating asialo-glycoprotein is now removed by this route and may be observed by radioautography of the 125I-labelled molecule (Thomas et al, 1977), or by immunoperoxidase staining (Heyderman & Neville, 1977; E. Heyderman & P. Thomas, un- This would give further support to the suggestion that biliary excretion occurs via a different mechanism from direct Kupffer-cell or parenchymal-cell uptake (Thomas & Summers, 1978).…”

Section: Methodsmentioning

confidence: 95%

The effects of triton WR1339 and asialo-fetuin on the hepatic uptake of circulating native and asialo-carcinoembryonic antigen

Thomas¹,

Jones²

1978

Biochemical Journal

View full text Add to dashboard Cite

Uptake of carcinoembryonic antigen from the circulation of the mouse is inhibited by treatment of the animal with Triton WR1339, but not by asialo-fetuin. Uptake of asialo-carcinoembryonic antigen is inhibited by asialo-fetuin, but not by Triton WR1339. These results reflect the different mechanisms of uptake of the glycoproteins. The presence or absence of sialic acid does not seem to be important in governing Kupffer-cell uptake, but when terminal galactoses are exposed on a glycoprotein, uptake by hepatocytes is preferred. Kupffer-cell uptake of carcinoembryonic antigen is not due to the formation of high-molecular-weight complexes in the blood stream. The effect of asialo-fetuin and Triton WR1339 on the biliary excretion of carcinoembryonic antigen and asialo-carcinoembryonic antigen is discussed.

show abstract

Section: Methodsmentioning

confidence: 95%

The effects of triton WR1339 and asialo-fetuin on the hepatic uptake of circulating native and asialo-carcinoembryonic antigen

Thomas¹,

Jones²

1978

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…Endogenous peroxidase was abolished by the hydrogen peroxide-periodic acidborohydride sequence (Heyderman & Neville, 1977 (Altmannsberger et al, 1981), gives a weak but specific staining of breast cancer metastatic cells also in formalin-fixed tissues.…”

Section: Methodsmentioning

confidence: 99%

The immunohistochemical detection of lymph node metastases from infiltrating lobular carcinoma of the breast

Bussolati¹,

Gugliotta²,

Morra³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

Summary Immunological markers improve specificity and accuracy of cell detection, therefore it is important to evaluate their usefulness in improving standard histological procedures.This study investigates whether immunocytochemical techniques increase the accuracy of detection, in axillary lymph nodes, of metastatic cells from infiltrating breast lobular carcinoma (ILC).Fifty cases of ILC reported to be node-negative were selected. New serial sections were cut from a total of 767 lymph nodes, stained with H&E and tested in immunoperoxidase (ABC procedure) with a conventional anti-Epithelial Membrane Antigen (EMA) serum, with a monoclonal raised against human milk fat globule membranes (HMFG-2) and with a monoclonal against 54kd keratin. Metastases were detected immunocytochemically in 12 cases (24%); in five of these cases metastatic cells were also visible in serial H&E sections. Monoclonals offered no evident advantage over anti-EMA conventional antiserum. Immunocytochemical positivity alone is not sufficient evidence for metastatic invasion since macrophages occasionally appear EMA-and HMFG-2-positive (probably because of secondary incorporation of the antigen), and so an improvement in the accuracy of breast cancer metastatic cell detection in axillary lymph nodes requires a combined histo-immunological approach.Prognosis and treatment of breast cancer is heavily influenced by the detection of metastases in lymph nodes. Foci formed by clumps of cells are easily spotted in standard histological sections, but a more difficult task is the detection of metastatic foci of one or few cells spread within a tissue.In as many as 24% of lymph nodes reported free of metastases by standard histological examination, various authors found metastases when multiple or serial sections were cut (Pickren, 1961;Fisher et al., 1978b). Immunological markers also improve the specificity and accuracy of cell detection, therefore it is important to evaluate their usefulness in improving standard histological procedures.Wells et al. (1984) utilizing 3 monoclonal antibodies found micro-metastatic foci in about 20% of lymph nodes from 45 cases of ductal or lobular breast cancer reported negative on conventional histology. On the contrary Sloane et al. (1980) reached the conclusion that detection of metastases in lymph nodes was not improved by immunocytochemistry. However they used a polyclonal antiserum against fat globule membranes of human milk.In this study 50 cases of infiltrating lobular carcinoma of the breast reported to have axillary lymph nodes free of metastases were re-examined to determine whether occult metastases were revealed

show abstract

“…-Approximately 4,um sections were dewaxed, bleached in a solution of hydrogen peroxide and treated with periodic acid, followed by borohydride (Heyderman & Neville, 1976). Sections were then incubated with a suitable dilution of rabbit antiserum for 90 min, -washed and incubated with the enzyme-conjugated second antibody for a further hour and a half.…”

mentioning

confidence: 99%

The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections

Sloane¹,

Ormerod²,

Imrie³

et al. 1980

Br J Cancer

101

View full text Add to dashboard Cite

We have investigated, by immunocytochemical means, the value of an antiserum raised to milk-fat-globule membranes in detecting metastatic deposits of breast carcinoma in conventional histological sections of liver, lymph nodes and marrow. The antiseum recognizes a membrane component, which we have called Epithelial Membrane Antigen, and which is confined to but widely distributed in epithelial tissues and tumours derived from them. In the sections examined, a greater extent of tumour infiltration was usually found, largely due to the identification of single malignant cells which normally go unrecognized with conventional stains. The number of positive samples was only increased, however, in sections of marrow aspirates, and the reasons for this are discussed. We suggest that further increases in detection rates could be attained by using the antiserum on cytological smears of marrow or even in cell suspensions. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4

show abstract

A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products.

Cited by 165 publications

References 12 publications

The effects of triton WR1339 and asialo-fetuin on the hepatic uptake of circulating native and asialo-carcinoembryonic antigen

The effects of triton WR1339 and asialo-fetuin on the hepatic uptake of circulating native and asialo-carcinoembryonic antigen

The immunohistochemical detection of lymph node metastases from infiltrating lobular carcinoma of the breast

The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections

Contact Info

Product

Resources

About