A signal-on fluorescence based biosensing platform for highly sensitive detection of DNA methyltransferase enzyme activity and inhibition

Dadmehr, Mehdi; Karimi, Mohammad Ali; Korouzhdehi, Behnaz

doi:10.1016/j.saa.2019.117731

Cited by 25 publications

(6 citation statements)

References 36 publications

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“…Thus, the integration of core–shell structures with graphene would enhance the performance of organic pollutant degradation and result in the increment of the photocatalytic properties. There are several reports on different biological applications of nanomaterials. − Among the different biological assays, cell cytotoxicity would be a crucial and determining factor for synthesized nanostructures, which was also discussed in the previous studies . In the present study, we constructed an effective and low-cost photocatalyst for RhB degradation.…”

Section: Introductionmentioning

confidence: 84%

“…There are several reports on different biological applications of nanomaterials. 49 − 53 Among the different biological assays, cell cytotoxicity would be a crucial and determining factor for synthesized nanostructures, which was also discussed in the previous studies. 54 In the present study, we constructed an effective and low-cost photocatalyst for RhB degradation.…”

Section: Introductionmentioning

confidence: 97%

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Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

et al. 2022

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Development of light-harvesting properties and inhibition of photogenerated charge carrier recombination are of paramount significance in the photocatalytic process. In the present work, we described the synthesis of core–shell heterostructures, which are composed of titanium oxide (TiO 2 ) and cerium oxide (CeO 2 ) deposited on a reduced graphene oxide (rGO) surface as a conductive substrate. Following the synthesis of ternary rGO-CeO 2 @TiO 2 and rGO-TiO 2 @CeO 2 nanostructures, their photocatalytic activity was investigated toward the degradation of rhodamine B dye as an organic pollutant under UV light irradiation. The obtained structures were characterized with high-resolution transmission electron microscopy, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Brunauer–Emmett–Teller, X-ray photoelectron spectroscopy surface analysis, and UV–Vis spectroscopy. Various parameters including pH, catalyst dosage, temperature, and contact time were studied for photocatalysis optimization. Heterostructures showed considerable advantages because of their high surface area and superior photocatalytic performance. In contrast, rGO-CeO 2 @TiO 2 showed the highest photocatalytic activity, which is attributed to the more effective electron–hole separation and quick suppression of charge recombination at core–shell phases. A biological assay of the prepared heterostructure was performed to determine the cytotoxicity against breast cancer cells (MCF-7) and demonstrated a very low survival rate at 7.65% of cells at the 17.5 mg mL –1 concentration of applied photocatalyst.

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Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 97%

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

et al. 2022

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“…In view of this, many methods have been developed to detect DNA MTase activity, such as colorimetric methods with double-stranded DNA (dsDNA) probes [ 7 ], electrochemistry with dsDNA probes and hairpin probes [ 8 , 9 , 10 ], fluorescence using dsDNA probes [ 11 , 12 , 13 ], hairpin probes and dumbbell probes [ 14 , 15 , 16 ], chemiluminescent immunoassays with dsDNA probes [ 17 ] and dumbbell probes [ 17 , 18 , 19 , 20 ], and surface-enhanced Raman scattering (SERS) using dsDNA probes [ 21 ] and hairpin probes [ 22 ]. In addition, these probes usually combine with other nucleotide probes to amplify signals, such as hybridization chain reaction (HCR) [ 23 , 24 ], strand displacement amplification(SDA) [ 25 ], and rolling circle amplification (RCA) [ 18 ], to detect DNA MTase activity precisely.…”

Section: Introductionmentioning

confidence: 99%

Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe

Wang

Han

Zhou³

et al. 2022

Biosensors

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DNA methyltransferases (MTases) can be regarded as biomarkers, as demonstrated by many studies on genetic diseases. Many researchers have developed biosensors to detect the activity of DNA MTases, and nucleic acid amplification, which need other probe assistance, is often used to improve the sensitivity of DNA MTases. However, there is no integrated probe that incorporates substrates and template and primer for detecting DNA MTases activity. Herein, we first designed a padlock probe (PP) to detect DNA MTases, which combines target detection with rolling circle amplification (RCA) without purification or other probe assistance. As the substrate of MTase, the PP was methylated and defended against HpaII, lambda exonuclease, and ExoI cleavage, as well as digestion, by adding MTase and the undestroyed PP started RCA. Thus, the fluorescent signal was capable of being rapidly detected after adding SYBRTM Gold to the RCA products. This method has a detection limit of approximately 0.0404 U/mL, and the linear range was 0.5–110 U/mL for M.SssI. Moreover, complex biological environment assays present prospects for possible application in intricacy environments. In addition, the designed detection system can also screen drugs or inhibitors for MTases.

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“…The various DNA template silver nanoclusters (DNA-Ag NCs) with fluorescence emission from the UV to near-infrared regions could also be synthesized based on changing the DNA sequence and environmental factors [ 7 ]. DNA-Ag NCs have been applied in the detection of a variety of targets, such as heavy metal ions [ 8 ], proteins [ 9 ], viruses, microRNAs [ 10 ], and thiols [ 11 ]. However, few studies have been involved in the application of DNA-Ag NCs in the enzyme-free fluorescent detection of pesticides.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters

Cheng

Huang

et al. 2022

Biosensors

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In this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu2+-ion-modulated strategy, was developed. In the presence of Cu2+, the fluorescence of the DNA-Ag NCs was quenched. Glyphosate can restore the fluorescence of DNA-Ag NCs. By analyzing the storage stability of the obtained DNA-Ag NCs using different DNA templates, specific DNA-Ag NCs were selected for the construction of the glyphosate sensor. The ultrasensitive detection of glyphosate was achieved by optimizing the buffer pH and Cu2+ concentration. The sensing of glyphosate demonstrated a linear response in the range of 1.0–50 ng/mL. The limit of detection (LOD) was 0.2 ng/mL. The proposed method was successfully applied in the detection of glyphosate in a real sample, indicating its high application potential for glyphosate detection.

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A signal-on fluorescence based biosensing platform for highly sensitive detection of DNA methyltransferase enzyme activity and inhibition

Cited by 25 publications

References 36 publications

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe

Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters

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A signal-on fluorescence based biosensing platform for highly sensitive detection of DNA methyltransferase enzyme activity and inhibition

Cited by 25 publications

References 36 publications

Fabrication of Graphene-Based TiO2@CeO2 and CeO2@TiO2 Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Fabrication of Graphene-Based TiO2@CeO2 and CeO2@TiO2 Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe

Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters

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Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity

Fabrication of Graphene-Based TiO₂@CeO₂ and CeO₂@TiO₂ Core–Shell Heterostructures for Enhanced Photocatalytic Activity and Cytotoxicity