A signal-on fluorescent aptasensor based on single-stranded DNA-sensitized luminescence of terbium (III) for label-free detection of breast cancer cells

Cai, Sanjun; Li, Guangwen; Xi, Zengzhe; Xia, Yaokun; Chen, Mei; Wu, Dongshuang; Chen, Qiuxiang; Zhang, Jing; Chen, Jinghua

doi:10.1016/j.talanta.2015.02.056

Cited by 28 publications

(10 citation statements)

References 55 publications

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“…The ds-DNA cannot increase the emission intensity of Tb 3+ compared to ssDNA. This signal-on aptasensor has a LOD of 70 cells/ mL (Cai et al 2015).…”

Section: Cancermentioning

confidence: 99%

Application of aptamers in diagnostics, drug-delivery and imaging

et al. 2016

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Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/ toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.

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“…The ds-DNA cannot increase the emission intensity of Tb 3+ compared to ssDNA. This signal-on aptasensor has a LOD of 70 cells/ mL (Cai et al 2015).…”

Section: Cancermentioning

confidence: 99%

Application of aptamers in diagnostics, drug-delivery and imaging

et al. 2016

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“…Therefore, the methodology had strong ability against interference and was considered to be a promising approach for monitoring CAP quantitatively. Another separation technology based fluorescence aptasensor was reported for facile and sensitive detection of MCF-7 cells . Excellent sensitivity with LOD of 70 cells/mL was displayed.…”

Section: Methods and Improvementsmentioning

confidence: 99%

Nucleic Acid Biosensors: Recent Advances and Perspectives

2016

Anal. Chem.

363

177

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“…In a research, the breast cancer diagnosis was followed by Cai et al 57 ; they designed an aptasensor that was able to detect breast cancer MCF-7 cells in the range of 0 to 500 cells 59 . In the mentioned research, the authors used of a specific MUC1 aptamer in order to detect of the MCF-7 cells; in Figure 1, by using the specific aptamer, the mentioned aptasensor was able to detect the MCF-7 cells while when the random DNA was used, the aptasensor did not work properly.…”

Section: Aptasensors For Detection the Breast Cancer Mcf-7 Cellsmentioning

confidence: 99%

Application of electrochemical aptasensors in detection of cancer biomarkers

Negahdary

Heli

2019

Biomed. Res. Ther.

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Today, the late diagnosis of cancers is a big challenge, and using novel diagnostic techniques will provide essential, faster and more accurate treatments. Unfortunately, existing common and traditional diagnostic methods have not been helpful completely and most cancers are diagnosed too late. Recently, researchers have found new diagnostic methods against cancers by aptasensors; these sensory systems can detect involved biomarkers in various cancers so that the research in this field is continued strictly. Aptasensors can detect cancer markers in small quantities and high selectivity; moreover, other advantages of cancer aptasensors such as optimized time and cost saving can be considered. In addition, the aptasensors have been used in the diagnosis of the effective and related factors in cancer therapy follow-up. Here, the most researches about cancer aptasensors and other involved markers were collected, reviewed and described.

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A signal-on fluorescent aptasensor based on single-stranded DNA-sensitized luminescence of terbium (III) for label-free detection of breast cancer cells

Cited by 28 publications

References 55 publications

Application of aptamers in diagnostics, drug-delivery and imaging

Application of aptamers in diagnostics, drug-delivery and imaging

Nucleic Acid Biosensors: Recent Advances and Perspectives

Application of electrochemical aptasensors in detection of cancer biomarkers

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