A signal‐switchable fluorescent probe for detection of HPO₄²⁻ based on 5,10,15,20‐(4‐sulphonatophenyl)porphyrin (TPPS₄)

Abstract

In this study, 5,10,15,20‐(4‐sulphonatophenyl)porphyrin (TPPS4) was selected as a fluorescent probe due to its excellent characteristics including high quantum yield, good water solubility, and exceptional biocompatibility. With an excitation wavelength set at 515 nm, the optimal fluorescence emission wavelength for TPPS4 was measured at 642 nm. At this moment, the fluorescence signal of TPPS4 pink solution was in the ‘ON’ state. The fluorescence intensity of TPPS4 was quenched when ascorbic acid (AA) was introduced, which was due to the electron transfer quenching effect between AA and TPPS4. The colour of the corresponding solution changed from pink to green, and the fluorescence signal was in the ‘OFF’ state. When HPO42− was further introduced into the TPPS4–AA system, the quenched fluorescence intensity of TPPS4 was recovered due to the unique interaction between HPO42− and AA. At this time, the colour of the corresponding solution changed from green to red, and the fluorescence signal was in the ‘ON’ state. Therefore, an ‘ON–OFF–ON’ signal‐switchable fluorescent probe was constructed based on TPPS4 to detect HPO42−. The results showed that the linear range of HPO42− was 4.0 × 10−9 to 1.7 × 10−6 M, and the detection limit was 1.3 × 10−9 M (S/N = 3). The sensing system exhibited high accuracy and sensitivity, and it could be used successfully to detect HPO42− in real samples.

Multifunctional Lanthanide Metal–Organic Frameworks Are Used for Fluorescence Sensing of Bi3+, HPO42–, Flu, and PNBA and Application

“…However, the excessive absorption of Bi 3+ by the human body can result in tissue necrosis and give rise to various diseases such as nephritis and methemoglobin leukemia, posing significant risks to human health . HPO 4 2– exists as a contaminant anion in aquatic environments and industrial production units, posing a serious threat to ecological balance and human health . With the rapid development of agriculture, pesticide residues are an important issue in the field of food safety, which may have a negative impact on human health and the environment .…”